FastQCFastQC Report
Fri 10 Feb 2017
ERR1631845.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631845.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916671
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA16250.17727188926015988No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA15430.1683264770021087No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA12680.13832661881962013No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA11480.125235771612716No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11200.12218124059777172No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10900.1189085287960457No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA10570.11530854581414705No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG10550.11509036502736533No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC10440.11389037070006577No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10360.11301764755293883No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10340.1127994667661571No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT9310.10156315624689774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11700.029.2521381
CCAATAC502.7015235E-422.23
TACGGCT1059.822543E-1021.14285930
GATAAGA450.003825225620.5555572
ATACGGC1001.2889359E-820.3529
GTATCAA17350.019.7262252
GCGGTAA6250.018.94400223
TAATAGA609.234872E-418.54
CGTTAGA801.6160884E-518.52
TATACTG500.00703382118.55
TATACAC2900.018.537
GACCGTT1156.40066E-817.6956527
CTTAGGA2100.017.619052
GTAACGT5200.017.43269226
AACGACC1705.456968E-1217.41176437
TCTATGG3200.017.343752
CGCGGTA6950.017.30215822
ATTAGTG752.0666917E-417.2666663
GGTAATA7300.017.23287625
CTCTATG3150.017.0317461
Produced by FastQC (version 0.11.5)