##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631845.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	916671
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67373136054266	34.0	31.0	34.0	31.0	34.0
2	32.83903712455177	34.0	31.0	34.0	31.0	34.0
3	32.93002069444763	34.0	31.0	34.0	31.0	34.0
4	36.32806863094829	37.0	37.0	37.0	35.0	37.0
5	36.25013663571772	37.0	37.0	37.0	35.0	37.0
6	36.30548473770851	37.0	37.0	37.0	35.0	37.0
7	36.293892792506796	37.0	37.0	37.0	35.0	37.0
8	36.272224167667574	37.0	37.0	37.0	35.0	37.0
9	38.11378018940274	39.0	39.0	39.0	37.0	39.0
10	38.048972859401026	39.0	38.0	39.0	35.0	39.0
11	38.11741289950265	39.0	38.0	39.0	37.0	39.0
12	38.06232225084027	39.0	38.0	39.0	37.0	39.0
13	38.10610677113163	39.0	38.0	39.0	37.0	39.0
14	39.53131385197088	41.0	39.0	41.0	37.0	41.0
15	39.536332010066864	41.0	39.0	41.0	37.0	41.0
16	39.4978525556061	41.0	39.0	41.0	37.0	41.0
17	39.46636143174596	41.0	39.0	41.0	37.0	41.0
18	39.47683956403115	41.0	39.0	41.0	37.0	41.0
19	39.50660160515605	41.0	39.0	41.0	37.0	41.0
20	39.490571862751196	41.0	39.0	41.0	37.0	41.0
21	39.462692721816225	41.0	39.0	41.0	37.0	41.0
22	39.44522734983435	41.0	39.0	41.0	37.0	41.0
23	39.388146892396506	41.0	39.0	41.0	37.0	41.0
24	39.397802483115534	41.0	39.0	41.0	36.0	41.0
25	39.34192747452467	41.0	39.0	41.0	36.0	41.0
26	39.294713152265096	40.0	39.0	41.0	36.0	41.0
27	39.19196309253811	40.0	39.0	41.0	36.0	41.0
28	39.19070091668657	40.0	39.0	41.0	36.0	41.0
29	39.169583198333974	40.0	39.0	41.0	36.0	41.0
30	39.131654650359835	40.0	39.0	41.0	35.0	41.0
31	39.05120593975374	40.0	39.0	41.0	35.0	41.0
32	39.000030545310146	40.0	39.0	41.0	35.0	41.0
33	38.94817988133147	40.0	38.0	41.0	35.0	41.0
34	38.91449167694844	40.0	38.0	41.0	35.0	41.0
35	38.837695312713066	40.0	38.0	41.0	35.0	41.0
36	38.7939009742863	40.0	38.0	41.0	35.0	41.0
37	38.7499637274442	40.0	38.0	41.0	35.0	41.0
38	38.704247216285886	40.0	38.0	41.0	35.0	41.0
39	38.65047983409533	40.0	38.0	41.0	35.0	41.0
40	38.343043469249054	40.0	38.0	41.0	34.0	41.0
41	38.403976999381456	40.0	38.0	41.0	34.0	41.0
42	38.39130505928517	40.0	38.0	41.0	34.0	41.0
43	37.93210977548106	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	1.0
12	2.0
13	1.0
14	3.0
15	0.0
16	2.0
17	4.0
18	6.0
19	14.0
20	26.0
21	63.0
22	110.0
23	243.0
24	450.0
25	680.0
26	1151.0
27	1799.0
28	2744.0
29	3820.0
30	5563.0
31	7759.0
32	10663.0
33	14479.0
34	21049.0
35	31755.0
36	49930.0
37	89191.0
38	209137.0
39	466023.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.782197756883335	17.070573848196354	12.728776191239824	27.418452203680495
2	20.255795154422906	19.928633064643694	33.467950878777664	26.347620902155732
3	20.948410062061527	20.55404828995354	29.42549726128567	29.072044386699265
4	15.416218032423847	15.999197094704643	34.14398404662087	34.44060082625064
5	16.490758407323895	33.577041272168536	33.647731847085815	16.28446847342176
6	33.843003651255465	34.08136616081451	15.644435135397542	16.43119505253248
7	29.92676761891671	28.38030220220777	20.34819471762497	21.344735461250547
8	26.795982418992203	31.820358667395393	20.214449895327768	21.16920901828464
9	27.764705112303105	13.590481208634287	18.223332035157654	40.421481643904954
10	18.719911505872883	24.250685360396478	30.89374486593336	26.13565826779728
11	37.74233067261864	20.36052193207814	19.881942376272402	22.01520501903082
12	22.472075586551775	24.303048749224093	27.348852532697116	25.876023131527013
13	33.38809671081555	18.409658427069253	22.102695514530296	26.099549347584905
14	23.851632701372687	20.365103728600555	23.625270135086634	32.157993434940124
15	28.43397467575608	24.93337304223653	20.958446378253484	25.67420590375391
16	25.3601346611816	24.720319503944165	23.019054819013583	26.900491015860652
17	26.054385924721085	24.39664830675346	22.809164902129552	26.739800866395903
18	25.464861438836834	22.763892388872346	24.590829206989202	27.180416965301617
19	27.250562088251947	23.10829076080731	24.385957448201154	25.25518970273959
20	28.263902752459714	22.131168107205312	23.964977620105795	25.639951520229175
21	26.405438810652893	23.3843985464796	23.031382033466752	27.178780609400754
22	26.924490902406646	23.80068748765915	22.510475405025357	26.76434620490885
23	26.796855142139332	23.246617379626933	23.520216086251228	26.43631139198251
24	26.949799873673324	22.98992768397822	23.400107563127882	26.66016487922057
25	27.12150815287055	22.996036746008112	23.833850967249973	26.04860413387137
26	26.934636308991994	23.60083388696708	23.355162321050845	26.10936748299008
27	26.533292751706995	22.78854681777868	24.1667948478789	26.511365582635428
28	25.651951463502172	23.74821500843814	23.386471263954025	27.213362264105662
29	26.32405737718331	24.14923129454297	23.39345304913104	26.13325827914268
30	25.97125904495724	23.514761566581686	24.406030080585072	26.107949307875998
31	26.85107306765459	23.31916249123186	23.717015155928355	26.1127492851852
32	24.77791923165454	23.31185343487467	24.119449617147264	27.79077771632352
33	25.24798973677579	22.998436734662707	25.163008320324305	26.590565208237198
34	25.71555116284905	22.790728625646498	24.725555842826925	26.768164368677528
35	25.4544978514647	23.975232117084538	24.83170079559624	25.73856923585452
36	25.51646119491072	23.157926889800155	25.280498674006267	26.04511324128286
37	26.064967692879996	22.51723900941559	24.921482189356922	26.496311108347488
38	25.228898917932383	22.271349262712576	26.03649510020498	26.463256719150053
39	24.555374829137172	21.665897579393263	26.83700040690717	26.941727184562403
40	24.568574766737466	22.271676533892748	27.41190678007704	25.747841919292746
41	23.29756259334047	21.69731561268983	28.279611769107998	26.7255100248617
42	22.35480341365659	22.04564123878687	28.532483300988034	27.06707204656851
43	21.703751945899892	22.440003010894856	28.577864904638634	27.278380138566614
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	17.5
2	16.0
3	35.5
4	55.0
5	55.0
6	72.0
7	89.0
8	104.5
9	120.0
10	180.5
11	241.0
12	241.0
13	479.5
14	718.0
15	1440.5
16	2163.0
17	2164.5
18	2166.0
19	2166.0
20	2563.5
21	2961.0
22	2885.0
23	2809.0
24	3228.0
25	3647.0
26	3647.0
27	4325.5
28	5004.0
29	7142.0
30	9280.0
31	10839.0
32	12398.0
33	12398.0
34	14978.0
35	17558.0
36	19653.0
37	21748.0
38	26486.5
39	31225.0
40	31225.0
41	36010.0
42	40795.0
43	44676.0
44	48557.0
45	58232.0
46	67907.0
47	67907.0
48	73453.0
49	78999.0
50	87629.0
51	96259.0
52	98718.5
53	101178.0
54	101178.0
55	91374.0
56	81570.0
57	75982.5
58	70395.0
59	64406.0
60	58417.0
61	58417.0
62	53949.0
63	49481.0
64	41557.0
65	33633.0
66	29370.0
67	25107.0
68	25107.0
69	21497.5
70	17888.0
71	15522.5
72	13157.0
73	10777.0
74	8397.0
75	8397.0
76	6721.0
77	5045.0
78	4169.5
79	3294.0
80	2616.0
81	1938.0
82	1938.0
83	1515.0
84	1092.0
85	891.0
86	690.0
87	526.5
88	363.0
89	363.0
90	293.0
91	223.0
92	142.0
93	61.0
94	33.5
95	6.0
96	6.0
97	3.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	916671.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.65564898915775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.559319494102	42.98159185539488
2	6.668644063130615	6.62271697664889
3	2.0770870619688764	3.0941731820714238
4	1.0320692842214052	2.049922804391575
5	0.6421143362505344	1.5942302045881274
6	0.4631482858209455	1.3798757226392984
7	0.3275614273058182	1.1385692679679653
8	0.25167096607460526	0.999750812173026
9	0.18149381111426155	0.8110973680554843
>10	1.4273080316226696	14.422417945714667
>50	0.21614302283850376	7.4488666832334305
>100	0.14195979654662963	13.07176098805723
>500	0.009051868829420374	2.95766448048098
>1k	0.0024285501737469294	1.4273617085830592
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1625	0.17727188926015988	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1543	0.1683264770021087	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1268	0.13832661881962013	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1148	0.125235771612716	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1120	0.12218124059777172	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1090	0.1189085287960457	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1057	0.11530854581414705	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1055	0.11509036502736533	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1044	0.11389037070006577	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1036	0.11301764755293883	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1034	0.1127994667661571	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	931	0.10156315624689774	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	1.0909039339086761E-4	0.0	0.0	0.0	0.0
9	1.0909039339086761E-4	0.0	0.0	0.0	0.0
10	1.0909039339086761E-4	0.0	0.0	0.0	0.0
11	1.0909039339086761E-4	0.0	0.0	2.1818078678173522E-4	0.0
12	1.0909039339086761E-4	0.0	0.0	3.272711801726028E-4	0.0
13	2.1818078678173522E-4	0.0	0.0	4.3636157356347045E-4	0.0
14	2.1818078678173522E-4	0.0	0.0	4.3636157356347045E-4	0.0
15	2.1818078678173522E-4	0.0	0.0	5.454519669543381E-4	0.0
16	3.272711801726028E-4	0.0	0.0	9.818135405178085E-4	0.0
17	3.272711801726028E-4	0.0	0.0	0.0013090847206904113	0.0
18	5.454519669543381E-4	0.0	0.0	0.001418175114081279	0.0
19	5.454519669543381E-4	0.0	0.0	0.0015272655074721464	0.0
20	5.454519669543381E-4	0.0	0.0	0.001963627081035617	0.0
21	5.454519669543381E-4	0.0	0.0	0.002945440621553425	0.0
22	5.454519669543381E-4	0.0	0.0	0.00501815809597991	0.0
23	5.454519669543381E-4	0.0	0.0	0.006545423603452056	1.0909039339086761E-4
24	5.454519669543381E-4	0.0	0.0	0.009163593044832878	1.0909039339086761E-4
25	5.454519669543381E-4	0.0	0.0	0.010254496978741555	1.0909039339086761E-4
26	5.454519669543381E-4	0.0	0.0	0.013309027993685848	1.0909039339086761E-4
27	5.454519669543381E-4	0.0	0.0	0.029018044641970782	1.0909039339086761E-4
28	5.454519669543381E-4	0.0	0.0	0.10570859119575071	1.0909039339086761E-4
29	5.454519669543381E-4	0.0	0.0	0.2094535553104658	1.0909039339086761E-4
30	6.545423603452056E-4	0.0	0.0	0.3548710497004923	1.0909039339086761E-4
31	6.545423603452056E-4	0.0	0.0	0.7685418214386623	1.0909039339086761E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1170	0.0	29.252138	1
CCAATAC	50	2.7015235E-4	22.2	3
TACGGCT	105	9.822543E-10	21.142859	30
GATAAGA	45	0.0038252256	20.555557	2
ATACGGC	100	1.2889359E-8	20.35	29
GTATCAA	1735	0.0	19.726225	2
GCGGTAA	625	0.0	18.944002	23
TAATAGA	60	9.234872E-4	18.5	4
CGTTAGA	80	1.6160884E-5	18.5	2
TATACTG	50	0.007033821	18.5	5
TATACAC	290	0.0	18.5	37
GACCGTT	115	6.40066E-8	17.695652	7
CTTAGGA	210	0.0	17.61905	2
GTAACGT	520	0.0	17.432692	26
AACGACC	170	5.456968E-12	17.411764	37
TCTATGG	320	0.0	17.34375	2
CGCGGTA	695	0.0	17.302158	22
ATTAGTG	75	2.0666917E-4	17.266666	3
GGTAATA	730	0.0	17.232876	25
CTCTATG	315	0.0	17.031746	1
>>END_MODULE