FastQCFastQC Report
Fri 10 Feb 2017
ERR1631844.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631844.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711017
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25380.3569534905635168No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25300.35582834165709115No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA20320.28578782223209853No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18570.2611751899040389No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC11880.16708461260419935No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11820.16624075092438015No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11770.16553753285786416No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA11660.16399045311152896No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA11630.1635685222716194No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA11450.1610369372321618No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA11440.16089629361885863No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA10990.1545673310202147No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG10720.15076995346102834No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA10480.14739450674175159No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA10320.14514420892890045No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC10250.14415970363577804No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT10210.14359712918256526No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG10150.14275326750274606No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC10030.14106554414310768No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA10000.14064361330319808No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA9830.13825267187704374No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC9580.13473658154446377No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC9450.13290821457152222No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9400.1322049965050062No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT8980.1262979647462719No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8910.1253134594531495No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA8640.12151608189396315No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA7870.1106865236696169No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA7790.10956137476319132No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT7640.10745172056364335No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC7400.1040762738443666No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7290.1025291940980314No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT7270.10224790687142501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13050.031.4712641
CCGCTTA604.317917E-827.75000225
TACGGCT400.00193046723.12530
GTCGTTG400.00193046723.1251
CTTTCGA652.6804555E-622.769236
GACCGTT603.7237125E-521.5833347
GGACCGT806.952432E-720.81256
ATACGGC450.003824393520.55555729
TAGGACC909.465475E-820.5555574
GTGTAGA555.1404414E-420.1818181
TTTCGAT759.257625E-619.7333347
AGTCGCC759.257625E-619.73333417
CTAGTTC1052.2557288E-819.380953
GTATCAA21100.019.3767782
CTTATAC6900.019.30434837
GCGGTAA4200.018.94047523
CGCGGTA4250.018.71764622
ACTATTC701.21836776E-418.58
CTAGACT701.21836776E-418.54
GTTCTAG500.007032306418.51
Produced by FastQC (version 0.11.5)