##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631844.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	711017
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6610193567805	34.0	31.0	34.0	31.0	34.0
2	32.817780728168245	34.0	31.0	34.0	31.0	34.0
3	32.906557789757485	34.0	31.0	34.0	31.0	34.0
4	36.30874929853998	37.0	37.0	37.0	35.0	37.0
5	36.23575948254402	37.0	35.0	37.0	35.0	37.0
6	36.2946272733282	37.0	37.0	37.0	35.0	37.0
7	36.28479206544991	37.0	37.0	37.0	35.0	37.0
8	36.271317000859334	37.0	37.0	37.0	35.0	37.0
9	38.0924028539402	39.0	38.0	39.0	37.0	39.0
10	38.04400598016644	39.0	38.0	39.0	35.0	39.0
11	38.11020552251212	39.0	38.0	39.0	37.0	39.0
12	38.05544593167252	39.0	38.0	39.0	35.0	39.0
13	38.10425489123326	39.0	38.0	39.0	37.0	39.0
14	39.49413727097946	41.0	39.0	41.0	37.0	41.0
15	39.50085440995082	41.0	39.0	41.0	37.0	41.0
16	39.44754626120051	41.0	39.0	41.0	37.0	41.0
17	39.431505857103275	41.0	39.0	41.0	37.0	41.0
18	39.46224633166295	41.0	39.0	41.0	37.0	41.0
19	39.4825749595298	41.0	39.0	41.0	37.0	41.0
20	39.486120585021176	41.0	39.0	41.0	37.0	41.0
21	39.45087388909126	41.0	39.0	41.0	37.0	41.0
22	39.423916727729434	41.0	39.0	41.0	37.0	41.0
23	39.3551687231107	41.0	39.0	41.0	36.0	41.0
24	39.35931630326701	41.0	39.0	41.0	36.0	41.0
25	39.298901432736486	41.0	39.0	41.0	36.0	41.0
26	39.23923900553714	40.0	39.0	41.0	36.0	41.0
27	39.12688585504988	40.0	39.0	41.0	35.0	41.0
28	39.12036702357327	40.0	39.0	41.0	35.0	41.0
29	39.09358426029195	40.0	39.0	41.0	35.0	41.0
30	39.02815966425557	40.0	39.0	41.0	35.0	41.0
31	38.93061206694073	40.0	38.0	41.0	35.0	41.0
32	38.87006639784984	40.0	38.0	41.0	35.0	41.0
33	38.799904924917406	40.0	38.0	41.0	35.0	41.0
34	38.76031374777256	40.0	38.0	41.0	35.0	41.0
35	38.684914706680715	40.0	38.0	41.0	35.0	41.0
36	38.61907099267669	40.0	38.0	41.0	35.0	41.0
37	38.56315671777187	40.0	38.0	41.0	35.0	41.0
38	38.51817185805684	40.0	38.0	41.0	35.0	41.0
39	38.44178409236348	40.0	38.0	41.0	35.0	41.0
40	38.11228845442514	40.0	37.0	41.0	34.0	41.0
41	38.15625786725212	40.0	37.0	41.0	34.0	41.0
42	38.11691984861122	40.0	37.0	41.0	34.0	41.0
43	37.6588646966247	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	0.0
15	0.0
16	1.0
17	1.0
18	10.0
19	18.0
20	26.0
21	56.0
22	91.0
23	210.0
24	320.0
25	578.0
26	900.0
27	1508.0
28	2372.0
29	3453.0
30	4753.0
31	6671.0
32	8803.0
33	11783.0
34	17513.0
35	26461.0
36	40369.0
37	75249.0
38	160906.0
39	348963.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.77647369894109	17.358516041107315	13.452561612450896	26.412448647500693
2	20.064780448287454	20.43087577371568	34.07879136504472	25.42555241295215
3	21.019609939002866	21.05448955510206	30.186620010492014	27.73928049540306
4	15.127064472438775	17.054866479985712	34.76949214997672	33.04857689759879
5	16.34110014247198	33.49933967823554	33.67500355125124	16.48455662804124
6	31.882641343315278	34.65557082320113	16.615777119253124	16.84601071423046
7	28.781449669979764	29.008870392691033	21.122139133100895	21.08754080422831
8	26.3291876284252	31.573647324888153	21.151111717441353	20.94605332924529
9	27.566851425493343	13.928358956255618	19.217121390909078	39.28766822734196
10	19.26325249607253	24.4162938438884	30.68871770998443	25.63173595005464
11	36.8705670891132	21.137609930564246	20.018227412284094	21.973595568038455
12	21.733094989289988	25.025140045877947	28.01620776999706	25.225557194835
13	33.96093201709664	18.567066610221698	22.572034142643567	24.8999672300381
14	23.203664609988227	21.80130714174204	25.17492549404585	29.820102754223882
15	28.69059389578589	25.27337602335809	21.782742184786017	24.253287896069995
16	23.729812367355493	25.20122584973355	24.28465142183661	26.784310361074347
17	24.90742134154317	24.974789632315403	23.87791009216376	26.239878933977668
18	25.42288018429939	22.439266571685344	25.25396720472225	26.883886039293014
19	26.76405768075869	23.567087706763694	25.281252065703068	24.387602546774552
20	28.11381443762948	21.953342887722798	25.061707385336778	24.871135289310946
21	26.230455811886355	23.600138955889943	24.1216454740182	26.0477597582055
22	26.43523291285581	24.106315320168154	23.67242977312779	25.786021993848244
23	26.260131614293332	23.3475430263974	24.677187746565835	25.715137612743437
24	26.246207896576312	22.939817191431427	24.89771693222525	25.91625797976701
25	26.313013612895332	23.465683661572086	25.252982699429126	24.968320026103456
26	26.180667972777023	24.010677663121978	24.686048294203935	25.122606069897063
27	25.668022002286868	23.311678905005085	25.88770732626646	25.132591766441593
28	24.790687142501515	24.513619224294214	24.401948195331478	26.293745437872794
29	25.597559552021963	24.774935057811557	24.101252150089238	25.52625324007724
30	25.33722822379774	24.152165138104998	25.527378388983667	24.983228249113594
31	26.26421027907912	23.476794507023037	24.694768198228733	25.564227015669104
32	23.98240829684804	23.86904954452566	24.918954117834033	27.229588040792272
33	24.189998270083557	23.42616280623389	26.862648853684227	25.52119006999833
34	24.233457146594244	23.333619308680383	25.928072043284477	26.504851501440896
35	24.387039972321336	24.845678795303066	26.34901837790095	24.418262854474644
36	24.645683577185917	23.976360621475997	26.374756159135437	25.00319964220265
37	25.169862323966935	23.618563269232663	25.869986230990254	25.34158817581014
38	24.17480875984681	23.00802934388348	26.625664365268342	26.191497531001367
39	23.8532974598357	22.03182202394598	27.898629709275585	26.216250806942732
40	23.70562166586734	23.13728082450912	28.24447235438815	24.91262515523539
41	22.675407198421414	22.349817233624513	28.851912120244666	26.12286344770941
42	21.353216589758052	22.57526894574954	29.67678691226792	26.394727552224488
43	20.790360849318652	23.346558521104278	29.205912094928816	26.657168534648257
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	24.0
1	22.0
2	20.0
3	66.5
4	113.0
5	113.0
6	171.5
7	230.0
8	251.0
9	272.0
10	391.0
11	510.0
12	510.0
13	1088.0
14	1666.0
15	3185.0
16	4704.0
17	4536.0
18	4368.0
19	4368.0
20	5086.5
21	5805.0
22	5102.5
23	4400.0
24	4586.0
25	4772.0
26	4772.0
27	5155.5
28	5539.0
29	6934.5
30	8330.0
31	9249.0
32	10168.0
33	10168.0
34	11974.5
35	13781.0
36	14854.0
37	15927.0
38	18237.0
39	20547.0
40	20547.0
41	24504.0
42	28461.0
43	31946.5
44	35432.0
45	47350.5
46	59269.0
47	59269.0
48	64987.5
49	70706.0
50	75876.0
51	81046.0
52	80332.0
53	79618.0
54	79618.0
55	70925.5
56	62233.0
57	57606.5
58	52980.0
59	47046.0
60	41112.0
61	41112.0
62	36753.0
63	32394.0
64	26697.5
65	21001.0
66	18258.0
67	15515.0
68	15515.0
69	13330.5
70	11146.0
71	9374.0
72	7602.0
73	6024.5
74	4447.0
75	4447.0
76	3573.0
77	2699.0
78	2205.5
79	1712.0
80	1389.0
81	1066.0
82	1066.0
83	862.0
84	658.0
85	525.5
86	393.0
87	310.0
88	227.0
89	227.0
90	161.5
91	96.0
92	59.5
93	23.0
94	13.5
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	711017.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.16691873258004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.72824035538146	37.86352224863221
2	6.506237427260748	5.747209194093922
3	2.162253569956029	2.8650023311043693
4	1.138634143540339	2.011598467355481
5	0.7525664822771734	1.6619271331799772
6	0.531120724528017	1.407477952645077
7	0.38237033102903417	1.1821683535416354
8	0.2915879399031727	1.030283267608309
9	0.23680090254937278	0.9412889596829775
>10	1.8520573272744638	16.39758021109512
>50	0.23856542562043637	7.2315182953563335
>100	0.1593642664782936	13.89782175119131
>500	0.014108707696267424	4.16015523770031
>1k	0.006092396505206388	3.6024465968130306
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2538	0.3569534905635168	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2530	0.35582834165709115	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2032	0.28578782223209853	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1857	0.2611751899040389	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	1188	0.16708461260419935	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1182	0.16624075092438015	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1177	0.16553753285786416	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1166	0.16399045311152896	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1163	0.1635685222716194	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	1145	0.1610369372321618	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1144	0.16089629361885863	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1099	0.1545673310202147	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	1072	0.15076995346102834	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1048	0.14739450674175159	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1032	0.14514420892890045	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	1025	0.14415970363577804	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1021	0.14359712918256526	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1015	0.14275326750274606	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	1003	0.14106554414310768	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	1000	0.14064361330319808	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	983	0.13825267187704374	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	958	0.13473658154446377	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	945	0.13290821457152222	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	940	0.1322049965050062	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	898	0.1262979647462719	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	891	0.1253134594531495	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	864	0.12151608189396315	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	787	0.1106865236696169	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	779	0.10956137476319132	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	764	0.10745172056364335	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	740	0.1040762738443666	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	729	0.1025291940980314	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	727	0.10224790687142501	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	2.812872266063962E-4	0.0	0.0	0.0	0.0
9	2.812872266063962E-4	0.0	0.0	0.0	0.0
10	2.812872266063962E-4	0.0	0.0	0.0	0.0
11	2.812872266063962E-4	0.0	0.0	0.0	0.0
12	2.812872266063962E-4	0.0	0.0	1.406436133031981E-4	0.0
13	2.812872266063962E-4	0.0	0.0	1.406436133031981E-4	0.0
14	2.812872266063962E-4	0.0	0.0	2.812872266063962E-4	0.0
15	2.812872266063962E-4	0.0	0.0	5.625744532127924E-4	0.0
16	2.812872266063962E-4	0.0	0.0	8.438616798191886E-4	0.0
17	5.625744532127924E-4	0.0	0.0	0.0012657925197287828	0.0
18	5.625744532127924E-4	0.0	0.0	0.0016877233596383772	0.0
19	5.625744532127924E-4	0.0	0.0	0.0023909414261543676	0.0
20	5.625744532127924E-4	0.0	0.0	0.0025315850394575655	0.0
21	5.625744532127924E-4	0.0	0.0	0.004781882852308735	0.0
22	5.625744532127924E-4	0.0	0.0	0.005766388145431122	0.0
23	5.625744532127924E-4	0.0	0.0	0.006750893438553509	0.0
24	5.625744532127924E-4	0.0	0.0	0.009704409317920668	0.0
25	5.625744532127924E-4	0.0	0.0	0.010266983771133461	0.0
26	5.625744532127924E-4	0.0	0.0	0.01336114326380382	0.0
27	5.625744532127924E-4	0.0	0.0	0.033613823579464346	0.0
28	5.625744532127924E-4	0.0	0.0	0.1248915286132399	0.0
29	5.625744532127924E-4	0.0	0.0	0.26173776435725166	0.0
30	5.625744532127924E-4	0.0	0.0	0.4441525308114996	0.0
31	5.625744532127924E-4	0.0	0.0	0.9379522571190281	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1305	0.0	31.471264	1
CCGCTTA	60	4.317917E-8	27.750002	25
TACGGCT	40	0.001930467	23.125	30
GTCGTTG	40	0.001930467	23.125	1
CTTTCGA	65	2.6804555E-6	22.76923	6
GACCGTT	60	3.7237125E-5	21.583334	7
GGACCGT	80	6.952432E-7	20.8125	6
ATACGGC	45	0.0038243935	20.555557	29
TAGGACC	90	9.465475E-8	20.555557	4
GTGTAGA	55	5.1404414E-4	20.181818	1
TTTCGAT	75	9.257625E-6	19.733334	7
AGTCGCC	75	9.257625E-6	19.733334	17
CTAGTTC	105	2.2557288E-8	19.38095	3
GTATCAA	2110	0.0	19.376778	2
CTTATAC	690	0.0	19.304348	37
GCGGTAA	420	0.0	18.940475	23
CGCGGTA	425	0.0	18.717646	22
ACTATTC	70	1.21836776E-4	18.5	8
CTAGACT	70	1.21836776E-4	18.5	4
GTTCTAG	50	0.0070323064	18.5	1
>>END_MODULE