FastQCFastQC Report
Fri 10 Feb 2017
ERR1631843.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631843.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164624
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39040.3352154858563794No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31550.2709028836774788No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30600.26274574454931376No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA24000.20607509376416766No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA15730.13506505103793157No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA15240.13085768454024646No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG14920.1281100166233909No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA14830.12733723502177527No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC14070.12081152371924328No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC13740.11797799117998599No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA13420.11523032326313042No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA12850.11033603978623144No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12720.10921979969500888No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12290.10552762093173418No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA12210.10484070395252029No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA12200.10475483933011856No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC12130.10415378697330642No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11780.10114852518924562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14700.031.5884341
CATACGC250.005496372529.63
CGAATTA400.001931202523.12515
ATACGGC1107.4578566E-1121.86363629
TATACTG603.7263417E-521.5833325
ATACACA2650.020.94339637
CCGAATT450.003825838720.55555514
GTTATGC450.003825838720.5555551
GTTATCG450.003825838720.55555511
TAAGGTA656.90223E-519.9230775
CGGCCTT3450.019.8405824
TCTAATA759.265943E-619.7333342
CTTATAC14600.019.64041137
CTACCGT951.6761624E-719.4736848
GCGGTAA4800.019.27083223
CGTGCCA5350.019.01869210
ATAGACC609.236918E-418.53
CGCCTAC500.00703493618.514
GTATAGA701.21921454E-418.51
CTCTATG3900.018.51
Produced by FastQC (version 0.11.5)