##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631843.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1164624
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.66461536083749	34.0	31.0	34.0	31.0	34.0
2	32.827625911882286	34.0	31.0	34.0	31.0	34.0
3	32.91202053194851	34.0	31.0	34.0	31.0	34.0
4	36.32351213782302	37.0	37.0	37.0	35.0	37.0
5	36.246087149157155	37.0	37.0	37.0	35.0	37.0
6	36.299631469040655	37.0	37.0	37.0	35.0	37.0
7	36.28243020923491	37.0	37.0	37.0	35.0	37.0
8	36.26350650510379	37.0	37.0	37.0	35.0	37.0
9	38.08962377557049	39.0	38.0	39.0	37.0	39.0
10	38.038475937298216	39.0	38.0	39.0	35.0	39.0
11	38.1014327370894	39.0	38.0	39.0	37.0	39.0
12	38.05522383189768	39.0	38.0	39.0	37.0	39.0
13	38.09373325639863	39.0	38.0	39.0	37.0	39.0
14	39.509104225913255	41.0	39.0	41.0	37.0	41.0
15	39.50611957163857	41.0	39.0	41.0	37.0	41.0
16	39.466844234705796	41.0	39.0	41.0	37.0	41.0
17	39.43629446070148	41.0	39.0	41.0	37.0	41.0
18	39.45891206088832	41.0	39.0	41.0	37.0	41.0
19	39.49430459959609	41.0	39.0	41.0	37.0	41.0
20	39.48207490142741	41.0	39.0	41.0	37.0	41.0
21	39.4511318674525	41.0	39.0	41.0	37.0	41.0
22	39.42828586737007	41.0	39.0	41.0	37.0	41.0
23	39.36200524804572	41.0	39.0	41.0	36.0	41.0
24	39.372248897498245	41.0	39.0	41.0	36.0	41.0
25	39.32229715341604	41.0	39.0	41.0	36.0	41.0
26	39.260839549931994	40.0	39.0	41.0	36.0	41.0
27	39.16491331107722	40.0	39.0	41.0	36.0	41.0
28	39.15260290016349	40.0	39.0	41.0	36.0	41.0
29	39.130086620231076	40.0	39.0	41.0	35.0	41.0
30	39.06892868427922	40.0	39.0	41.0	35.0	41.0
31	38.97551913750704	40.0	38.0	41.0	35.0	41.0
32	38.92569447306599	40.0	38.0	41.0	35.0	41.0
33	38.86796940471775	40.0	38.0	41.0	35.0	41.0
34	38.82695874376623	40.0	38.0	41.0	35.0	41.0
35	38.759556732473314	40.0	38.0	41.0	35.0	41.0
36	38.70549636620918	40.0	38.0	41.0	35.0	41.0
37	38.661498475044304	40.0	38.0	41.0	35.0	41.0
38	38.61628645811867	40.0	38.0	41.0	35.0	41.0
39	38.558991571528665	40.0	38.0	41.0	35.0	41.0
40	38.237010399923065	40.0	38.0	41.0	34.0	41.0
41	38.307424542169834	40.0	38.0	41.0	34.0	41.0
42	38.28348720273668	40.0	38.0	41.0	34.0	41.0
43	37.81254980148099	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	1.0
14	2.0
15	1.0
16	1.0
17	2.0
18	1.0
19	11.0
20	40.0
21	85.0
22	150.0
23	328.0
24	531.0
25	843.0
26	1530.0
27	2295.0
28	3436.0
29	5337.0
30	7583.0
31	10403.0
32	13869.0
33	19131.0
34	27924.0
35	41910.0
36	64268.0
37	120711.0
38	264114.0
39	580114.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.53415694679141	17.211477695805684	13.512944950473285	26.741420406929617
2	19.029231752050446	20.298997788127327	35.230683894544505	25.441086565277722
3	20.06922405858028	21.039923614831913	30.430508043797825	28.460344282789983
4	14.91597288051766	16.582175878223357	34.27380854249955	34.228042698759424
5	15.77436151066782	34.488985286238304	33.74402382228084	15.992629380813034
6	32.957418016458526	34.59708884584209	16.282508346041297	16.16298479165808
7	29.397041448570526	29.390859195757603	20.686504829026365	20.52559452664551
8	26.320340298671503	33.14314319471349	20.60656486557035	19.929951641044664
9	27.514631331657256	14.319643077937599	18.585569248100676	39.58015634230447
10	18.492577862039596	25.255704845512373	30.779376004616083	25.472341287831952
11	36.88211817719711	21.133086730137794	20.270319004245145	21.714476088419953
12	22.049691574276334	24.75537169077745	27.91038137630686	25.284555358639356
13	32.87249790490321	18.60463119427386	23.333968731539105	25.18890216928382
14	23.253857038838284	20.849733476212066	24.921519735124814	30.974889749824836
15	27.8036516506615	25.629988734561543	21.891099616700327	24.675259998076633
16	24.470129415158883	25.404508236134582	23.48594911319018	26.639413235516358
17	25.344403000453365	25.074530492244705	23.61148319114152	25.96958331616041
18	25.128281745868193	23.152536784404237	25.149146849111816	26.57003462061575
19	26.914265891824314	23.697261948920854	25.070580719614227	24.317891439640604
20	27.74552130129553	22.315184986742505	25.131287007652254	24.80800670430972
21	25.81528458970449	24.066393960625916	23.831640082979572	26.286681366690022
22	26.228551017324047	24.233143057330093	23.422323427990495	26.115982497355372
23	25.711044938108778	24.05952479083378	24.523966533404774	25.705463737652668
24	26.45815301762629	23.24329569028287	24.769367624228934	25.529183667861904
25	26.249931308302077	23.55713088516122	25.147944744398192	25.04499306213851
26	26.136504142109384	24.044326752668674	24.534613746582586	25.284555358639356
27	25.830053304757588	23.307608292461772	25.403821319155366	25.45851708362527
28	24.744381019110033	24.549124867768484	24.387355919163607	26.31913819395788
29	25.648707222245122	24.83136188160299	24.07188929645963	25.44804159969226
30	25.012278641003448	24.713641484290207	25.11016431054143	25.163915564164913
31	26.1275742213796	23.687387517344654	24.671310225446152	25.51372803582959
32	23.678543461237275	24.04175081399662	25.137211666597974	27.142494058168133
33	24.336781656568988	23.29550138070313	26.4390052068307	25.928711755897183
34	24.46351783923395	23.272575526521866	26.022218329692677	26.241688304551513
35	24.54414471966918	24.31806316888541	26.207600049458023	24.930192061987388
36	24.46781107035404	23.720617126214126	26.589010702166537	25.2225611012653
37	25.171299921691464	22.939678385470334	25.896770116363736	25.992251576474466
38	24.115594389262114	22.555691794089768	27.0026205882757	26.32609322837242
39	23.721561637060546	21.60989297833464	28.18085493687233	26.487690447732486
40	23.400170355410847	22.59098215389688	28.74773317396859	25.26111431672368
41	22.176857080053306	22.013199109755597	29.64012419458984	26.16981961560126
42	21.6821909903969	21.942532525518963	29.753036173048127	26.62224031103601
43	20.54688895300114	22.843939331492397	29.968470510654083	26.64070120485238
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	36.0
1	38.0
2	40.0
3	95.0
4	150.0
5	150.0
6	231.5
7	313.0
8	326.0
9	339.0
10	554.0
11	769.0
12	769.0
13	1471.0
14	2173.0
15	4619.5
16	7066.0
17	6976.0
18	6886.0
19	6886.0
20	7284.5
21	7683.0
22	6925.0
23	6167.0
24	6578.5
25	6990.0
26	6990.0
27	7609.5
28	8229.0
29	11360.0
30	14491.0
31	16169.0
32	17847.0
33	17847.0
34	20840.5
35	23834.0
36	25625.0
37	27416.0
38	33938.0
39	40460.0
40	40460.0
41	47187.0
42	53914.0
43	57012.5
44	60111.0
45	77315.0
46	94519.0
47	94519.0
48	103183.5
49	111848.0
50	121093.0
51	130338.0
52	131955.0
53	133572.0
54	133572.0
55	116658.0
56	99744.0
57	91460.0
58	83176.0
59	73344.0
60	63512.0
61	63512.0
62	57158.0
63	50804.0
64	42546.5
65	34289.0
66	29802.0
67	25315.0
68	25315.0
69	21774.5
70	18234.0
71	15783.0
72	13332.0
73	10774.5
74	8217.0
75	8217.0
76	6774.5
77	5332.0
78	4324.0
79	3316.0
80	2579.5
81	1843.0
82	1843.0
83	1492.0
84	1141.0
85	880.5
86	620.0
87	483.5
88	347.0
89	347.0
90	257.5
91	168.0
92	102.0
93	36.0
94	21.0
95	6.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1164624.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.54534807266311
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.82525804301396	36.93745386836509
2	7.407078123765087	6.4508759020151825
3	2.3434035953804946	3.061329757067215
4	1.24457431878282	2.167816876547819
5	0.7480638934662325	1.6287351310789333
6	0.5068351659266409	1.3242188229444927
7	0.3742761402327154	1.140858936120852
8	0.2847014622508817	0.9917939416408641
9	0.2195707773109223	0.8605157332130381
>10	1.6078160271632285	14.024540025171417
>50	0.22487253193053755	6.89100102320713
>100	0.19171860925163905	16.297028286956554
>500	0.01627425040942557	4.556907434470701
>1k	0.0055570611154136095	3.666924261200725
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3904	0.3352154858563794	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3155	0.2709028836774788	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3060	0.26274574454931376	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2400	0.20607509376416766	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1573	0.13506505103793157	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1524	0.13085768454024646	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1492	0.1281100166233909	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	1483	0.12733723502177527	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	1407	0.12081152371924328	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1374	0.11797799117998599	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1342	0.11523032326313042	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1285	0.11033603978623144	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1272	0.10921979969500888	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1229	0.10552762093173418	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1221	0.10484070395252029	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1220	0.10475483933011856	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1213	0.10415378697330642	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1178	0.10114852518924562	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	8.586462240173653E-5	0.0	0.0	0.0	0.0
9	8.586462240173653E-5	0.0	0.0	0.0	0.0
10	8.586462240173653E-5	0.0	0.0	0.0	0.0
11	8.586462240173653E-5	0.0	0.0	0.0	0.0
12	8.586462240173653E-5	0.0	0.0	8.586462240173653E-5	0.0
13	8.586462240173653E-5	0.0	0.0	1.7172924480347306E-4	0.0
14	8.586462240173653E-5	0.0	0.0	4.293231120086826E-4	0.0
15	8.586462240173653E-5	0.0	0.0	6.869169792138922E-4	0.0
16	8.586462240173653E-5	0.0	0.0	0.001631427825632994	0.0
17	8.586462240173653E-5	0.0	0.0	0.0024900740496503595	0.0
18	8.586462240173653E-5	0.0	0.0	0.003091126406462515	0.0
19	8.586462240173653E-5	0.0	0.0	0.004121501875283353	0.0
20	8.586462240173653E-5	0.0	0.0	0.00566706507851461	0.0
21	8.586462240173653E-5	0.0	0.0	0.009359243841789282	0.0
22	2.575938672052096E-4	0.0	0.0	0.01356661033947437	0.0
23	2.575938672052096E-4	0.0	0.0	0.016400142878731677	0.0
24	2.575938672052096E-4	0.0	0.0	0.019834727774801136	0.0
25	2.575938672052096E-4	0.0	0.0	0.02146615560043413	0.0
26	2.575938672052096E-4	0.0	0.0	0.024385552762093175	0.0
27	2.575938672052096E-4	0.0	0.0	0.042159529599252635	0.0
28	3.434584896069461E-4	0.0	0.0	0.13205978925387077	0.0
29	3.434584896069461E-4	0.0	0.0	0.2863585157097913	0.0
30	4.293231120086826E-4	0.0	0.0	0.49028699391391556	0.0
31	4.293231120086826E-4	0.0	0.0	1.1603745071370675	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1470	0.0	31.588434	1
CATACGC	25	0.0054963725	29.6	3
CGAATTA	40	0.0019312025	23.125	15
ATACGGC	110	7.4578566E-11	21.863636	29
TATACTG	60	3.7263417E-5	21.583332	5
ATACACA	265	0.0	20.943396	37
CCGAATT	45	0.0038258387	20.555555	14
GTTATGC	45	0.0038258387	20.555555	1
GTTATCG	45	0.0038258387	20.555555	11
TAAGGTA	65	6.90223E-5	19.923077	5
CGGCCTT	345	0.0	19.84058	24
TCTAATA	75	9.265943E-6	19.733334	2
CTTATAC	1460	0.0	19.640411	37
CTACCGT	95	1.6761624E-7	19.473684	8
GCGGTAA	480	0.0	19.270832	23
CGTGCCA	535	0.0	19.018692	10
ATAGACC	60	9.236918E-4	18.5	3
CGCCTAC	50	0.007034936	18.5	14
GTATAGA	70	1.21921454E-4	18.5	1
CTCTATG	390	0.0	18.5	1
>>END_MODULE