FastQCFastQC Report
Fri 10 Feb 2017
ERR1631842.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631842.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences885649
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA18280.2064023106219281No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA17070.1927400132558158No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16070.18144885840778907No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA15960.18020683137450616No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG15890.17941645053514427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15650.17670657337161788No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA15190.1715126421415256No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC15130.170835172850644No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA14820.1673349148477557No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14530.16406047994182799No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT14200.16033439884197917No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC13530.15276932509380126No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT13090.1478012169606695No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12550.1417039933427351No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA12310.13899411617920868No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA9760.11020167131674061No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC9520.1074917941532142No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA9460.10681432486233261No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA9290.10489482853816806No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA9020.10184621672920086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12100.029.9669441
TCCTATA250.005495671629.5999982
AATCTAC250.005495671629.5999981
ATACGGC652.9885996E-928.46153829
GTACTAT706.5883796E-926.428571
CCGACAA601.3368281E-624.66666814
CGACAAG701.9218896E-723.78571315
TACCCGA652.6816924E-622.7692311
GATTGTA502.7014242E-422.19999923
GCCTATA603.7250422E-521.58333437
CTTATAC10250.020.756137
GGGCTAA450.00382512520.5555551
TATACTT450.00382512520.5555555
TACGGCT1001.288754E-820.34999830
TGATTGT555.141813E-420.18181822
TAACCCT1750.020.08571435
CCTAGTT656.899846E-519.9230772
TATATCT656.899846E-519.9230775
TATCTAT656.899846E-519.9230777
TCGCTAC656.899846E-519.92307727