##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631842.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	885649
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.68001092983789	34.0	31.0	34.0	31.0	34.0
2	32.854167960444826	34.0	31.0	34.0	31.0	34.0
3	32.939162128563346	34.0	31.0	34.0	31.0	34.0
4	36.34286720811518	37.0	37.0	37.0	35.0	37.0
5	36.26766021301893	37.0	37.0	37.0	35.0	37.0
6	36.31452753856212	37.0	37.0	37.0	35.0	37.0
7	36.30651307685099	37.0	37.0	37.0	35.0	37.0
8	36.289207123815416	37.0	37.0	37.0	35.0	37.0
9	38.12138104373177	39.0	39.0	39.0	37.0	39.0
10	38.066285853650825	39.0	38.0	39.0	37.0	39.0
11	38.13097175065968	39.0	38.0	39.0	37.0	39.0
12	38.078962433198704	39.0	38.0	39.0	37.0	39.0
13	38.11983076817114	39.0	38.0	39.0	37.0	39.0
14	39.53861744325348	41.0	39.0	41.0	37.0	41.0
15	39.544164787630315	41.0	39.0	41.0	37.0	41.0
16	39.4991119506712	41.0	39.0	41.0	37.0	41.0
17	39.46476312850802	41.0	39.0	41.0	37.0	41.0
18	39.48814372285183	41.0	39.0	41.0	37.0	41.0
19	39.51857564339823	41.0	39.0	41.0	37.0	41.0
20	39.51815674155337	41.0	39.0	41.0	37.0	41.0
21	39.48086770266776	41.0	39.0	41.0	37.0	41.0
22	39.458475084373156	41.0	39.0	41.0	37.0	41.0
23	39.395487377053435	41.0	39.0	41.0	37.0	41.0
24	39.41193858966701	41.0	39.0	41.0	37.0	41.0
25	39.36436782517679	41.0	39.0	41.0	36.0	41.0
26	39.32253748381131	41.0	39.0	41.0	36.0	41.0
27	39.21848949188674	40.0	39.0	41.0	36.0	41.0
28	39.21508633781554	40.0	39.0	41.0	36.0	41.0
29	39.20482606540514	40.0	39.0	41.0	36.0	41.0
30	39.161701757694075	40.0	39.0	41.0	36.0	41.0
31	39.08179538395007	40.0	39.0	41.0	35.0	41.0
32	39.03779826996925	40.0	39.0	41.0	35.0	41.0
33	38.98217239561045	40.0	39.0	41.0	35.0	41.0
34	38.958045455931185	40.0	38.0	41.0	35.0	41.0
35	38.89258950216169	40.0	38.0	41.0	35.0	41.0
36	38.849246146046575	40.0	38.0	41.0	35.0	41.0
37	38.808673639331154	40.0	38.0	41.0	35.0	41.0
38	38.77061115633846	40.0	38.0	41.0	35.0	41.0
39	38.70973150762887	40.0	38.0	41.0	35.0	41.0
40	38.41322578131969	40.0	38.0	41.0	35.0	41.0
41	38.48118498411899	40.0	38.0	41.0	35.0	41.0
42	38.47070227595808	40.0	38.0	41.0	35.0	41.0
43	38.0250381358755	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	3.0
17	2.0
18	5.0
19	12.0
20	24.0
21	65.0
22	112.0
23	217.0
24	322.0
25	596.0
26	950.0
27	1558.0
28	2453.0
29	3701.0
30	5329.0
31	7448.0
32	9909.0
33	13641.0
34	20033.0
35	30620.0
36	47317.0
37	86805.0
38	196086.0
39	458441.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.140283566062855	17.031352149666514	13.550514933116844	27.277849351153787
2	19.635431192266914	20.133935678807294	34.25149240839204	25.979140720533756
3	20.642940939356336	20.68212124667899	30.27203779375351	28.40290002021117
4	15.195862017571296	16.797963978957803	34.228232629405106	33.777941374065804
5	16.16735298069551	33.64256042743796	33.90733800862418	16.282748583242345
6	32.7817227818244	34.113627407697635	16.375561876093126	16.72908793438484
7	29.193958328863918	28.879612577894854	20.790290510123086	21.136138583118143
8	26.352426299809515	31.98050243380843	20.871812648125836	20.795258618256216
9	27.905863383801034	13.999338338325906	18.55859375441061	39.53620452346245
10	18.900264100111897	24.616975799667813	30.448970190222084	26.033789909998205
11	37.29073255883539	20.626455853278216	19.899418392613775	22.183393195272618
12	22.12174348980239	24.794811488524235	27.301786599431605	25.781658422241772
13	33.58203983745254	18.49999266074935	22.38347245917965	25.534495042618467
14	23.43524353327334	21.2027563967215	24.182492161115746	31.179507908889413
15	28.655596065710004	25.412211835614336	21.111749688646405	24.820442410029255
16	24.693529829537436	25.187630765687086	23.302911198454467	26.815928206321015
17	25.878084884643915	24.884801992663007	22.80971355469266	26.427399568000414
18	25.443601246091852	22.69420504059735	24.671850812229223	27.190342901081582
19	27.011265185191874	23.517894786760895	24.71272479277908	24.758115235268146
20	28.54449110200542	21.91748649860159	24.36077949616609	25.177242903226897
21	26.11147305535263	23.75681562334514	23.16098138201477	26.97072993928746
22	26.835461904208103	23.90472975185429	22.704367079960573	26.555441263977038
23	26.323633854947047	23.50795857049463	23.930925231101714	26.23748234345661
24	27.00957151196467	22.972983653795126	24.152683512316955	25.864761321923247
25	26.578813954512455	23.099670411189983	24.524162506817035	25.797353127480527
26	26.586830674454554	23.801189861897885	23.778494640653353	25.833484822994212
27	25.875262095931912	22.888299992434927	25.196324954920062	26.040112956713102
28	25.016456858191	24.11192244331558	23.736604456167175	27.135016242326248
29	26.057727158276023	24.643397102012194	23.512813767079283	25.786061972632503
30	25.5245588263522	23.73615280997325	24.781939571997487	25.957348791677067
31	26.56357089546762	23.476117513823198	24.249335797816066	25.71097579289312
32	24.35694050351776	23.539122157875187	24.306920687541	27.797016651066052
33	24.6948847681192	22.978629231219138	25.986705794281935	26.339780206379725
34	25.14314361558586	22.96157958739862	25.187630765687086	26.707646031328437
35	24.6954493258616	24.3406812405366	25.569836357292786	25.394033076309015
36	24.991503405976857	23.34694670236177	25.956558410837705	25.70499148082367
37	25.60472602577319	22.78125984447563	25.56249710664157	26.051517023109604
38	24.655930283893507	22.280158392320207	26.70877514681324	26.355136176973044
39	24.286822431911514	21.27863295730024	27.708493997057527	26.726050613730724
40	23.946055378598068	22.27304496476595	28.251146899053687	25.52975275758229
41	22.37489118149515	21.749699937559914	29.197345675318324	26.678063205626607
42	21.43919317923918	21.86317604378258	29.53009600868967	27.167534768288565
43	20.503269353886246	22.75879044632806	29.550420087416118	27.187520112369572
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15.0
1	16.5
2	18.0
3	46.5
4	75.0
5	75.0
6	108.5
7	142.0
8	146.5
9	151.0
10	270.5
11	390.0
12	390.0
13	728.5
14	1067.0
15	2117.5
16	3168.0
17	3184.5
18	3201.0
19	3201.0
20	3595.0
21	3989.0
22	3572.0
23	3155.0
24	3460.0
25	3765.0
26	3765.0
27	4191.0
28	4617.0
29	7031.0
30	9445.0
31	10251.5
32	11058.0
33	11058.0
34	13058.5
35	15059.0
36	16315.0
37	17571.0
38	22787.0
39	28003.0
40	28003.0
41	33493.5
42	38984.0
43	41847.5
44	44711.0
45	59143.0
46	73575.0
47	73575.0
48	80360.5
49	87146.0
50	97192.0
51	107238.0
52	107560.5
53	107883.0
54	107883.0
55	93046.0
56	78209.0
57	71238.5
58	64268.0
59	57915.0
60	51562.0
61	51562.0
62	46649.0
63	41736.0
64	34369.5
65	27003.0
66	23257.0
67	19511.0
68	19511.0
69	16570.0
70	13629.0
71	11741.0
72	9853.0
73	7881.5
74	5910.0
75	5910.0
76	4825.5
77	3741.0
78	3079.5
79	2418.0
80	1941.0
81	1464.0
82	1464.0
83	1167.0
84	870.0
85	705.5
86	541.0
87	412.0
88	283.0
89	283.0
90	233.0
91	183.0
92	106.5
93	30.0
94	19.0
95	8.0
96	8.0
97	5.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	885649.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.1012236440414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.56019163762376	34.34426124236932
2	7.880615872004274	6.4780591081606
3	2.5640592984150468	3.1615792398222218
4	1.3225062676526915	2.17426503509759
5	0.7804860181228779	1.6039465190957878
6	0.5519117606666162	1.3610549224161146
7	0.406247341033296	1.1688083973024659
8	0.3025420218199438	0.9947877840433569
9	0.2305513138914861	0.8528347002313403
>10	1.8822580270189797	15.919488260283051
>50	0.27892272810035984	8.00123561205327
>100	0.22037644642086746	17.113456366615555
>500	0.015188852823477374	4.280634202330664
>1k	0.0041424144064029205	2.5455886101787133
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1828	0.2064023106219281	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1707	0.1927400132558158	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1607	0.18144885840778907	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1596	0.18020683137450616	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1589	0.17941645053514427	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1565	0.17670657337161788	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1519	0.1715126421415256	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1513	0.170835172850644	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1482	0.1673349148477557	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1453	0.16406047994182799	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1420	0.16033439884197917	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1353	0.15276932509380126	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1309	0.1478012169606695	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1255	0.1417039933427351	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1231	0.13899411617920868	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	976	0.11020167131674061	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	952	0.1074917941532142	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	946	0.10681432486233261	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	929	0.10489482853816806	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	902	0.10184621672920086	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	1.1291154848026701E-4	0.0	0.0	0.0	0.0
9	1.1291154848026701E-4	0.0	0.0	0.0	0.0
10	1.1291154848026701E-4	0.0	0.0	0.0	0.0
11	1.1291154848026701E-4	0.0	0.0	0.0	0.0
12	1.1291154848026701E-4	0.0	0.0	0.0	0.0
13	1.1291154848026701E-4	0.0	0.0	0.0	0.0
14	1.1291154848026701E-4	0.0	0.0	3.3873464544080105E-4	0.0
15	1.1291154848026701E-4	0.0	0.0	4.5164619392106805E-4	0.0
16	1.1291154848026701E-4	0.0	0.0	5.645577424013351E-4	0.0
17	1.1291154848026701E-4	0.0	0.0	0.001016203936322403	0.0
18	4.5164619392106805E-4	0.0	0.0	0.001242027033282937	0.0
19	4.5164619392106805E-4	0.0	0.0	0.0019194963241645393	0.0
20	4.5164619392106805E-4	0.0	0.0	0.0025969656150461413	0.0
21	4.5164619392106805E-4	0.0	0.0	0.005081019681612016	0.0
22	5.645577424013351E-4	0.0	0.0	0.00756507374817789	0.0
23	5.645577424013351E-4	0.0	0.0	0.008920012329941093	0.0
24	5.645577424013351E-4	0.0	0.0	0.011065331751066167	0.0
25	5.645577424013351E-4	0.0	0.0	0.012646093429789906	0.0
26	5.645577424013351E-4	0.0	0.0	0.01614635143267818	0.0
27	5.645577424013351E-4	0.0	0.0	0.03658334170760651	0.0
28	5.645577424013351E-4	0.0	0.0	0.12950954610686627	0.0
29	6.774692908816021E-4	0.0	0.0	0.2784398785523385	0.0
30	6.774692908816021E-4	0.0	0.0	0.4777287616200097	0.0
31	6.774692908816021E-4	0.0	0.0	1.0468029659605556	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1210	0.0	29.966944	1
TCCTATA	25	0.0054956716	29.599998	2
AATCTAC	25	0.0054956716	29.599998	1
ATACGGC	65	2.9885996E-9	28.461538	29
GTACTAT	70	6.5883796E-9	26.42857	1
CCGACAA	60	1.3368281E-6	24.666668	14
CGACAAG	70	1.9218896E-7	23.785713	15
TACCCGA	65	2.6816924E-6	22.76923	11
GATTGTA	50	2.7014242E-4	22.199999	23
GCCTATA	60	3.7250422E-5	21.583334	37
CTTATAC	1025	0.0	20.7561	37
GGGCTAA	45	0.003825125	20.555555	1
TATACTT	45	0.003825125	20.555555	5
TACGGCT	100	1.288754E-8	20.349998	30
TGATTGT	55	5.141813E-4	20.181818	22
TAACCCT	175	0.0	20.085714	35
CCTAGTT	65	6.899846E-5	19.923077	2
TATATCT	65	6.899846E-5	19.923077	5
TATCTAT	65	6.899846E-5	19.923077	7
TCGCTAC	65	6.899846E-5	19.923077	27
>>END_MODULE