FastQCFastQC Report
Fri 10 Feb 2017
ERR1631841.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631841.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690492
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA17080.24735985355369794No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA16300.23606356047571878No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14610.21158825880676388No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA14240.2062297608082353No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG13850.2005816142692457No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC13590.19681618324325265No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA13210.19131286097449357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13100.18971979400195801No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT12790.18523024162481247No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA12550.1817544591392804No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC12000.17378912427660276No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11220.1624928311986236No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA11120.16104458849631856No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT11060.16017564287493555No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10480.1517758352015664No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA8400.12165238699362194No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA8320.12049379283177793No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA8190.11861107731878139No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT7400.10716995997057172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10400.030.7740381
TACGGCT706.5829227E-926.42857230
GTGTTGA551.9012328E-523.5454541
TAGCGAA551.9012328E-523.54545410
CTATATG400.0019304123.1250021
ATTGGAG806.951832E-720.81253
CTTATAC9300.019.89247337
TAAGGTA851.243834E-619.5882365
ATACGGC951.6736885E-719.47368229
GTATCAA16800.018.9404752
GTAGCGA701.2183026E-418.59
ATTAGAG500.007032108518.4999983
GGACGTA500.007032108518.49999827
TTACGAG500.007032108518.4999983
CAGTCGG2250.018.08888810
GCAGTCG2300.017.6956529
TAAAGAC2550.017.41176437
TAGTTCC852.7205655E-517.4117644
ACGGTAC1603.45608E-1117.343753
GTAGAAC1502.5102054E-1017.2666663
Produced by FastQC (version 0.11.5)