##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631841.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	690492
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.679138932818915	34.0	31.0	34.0	31.0	34.0
2	32.83957525938027	34.0	31.0	34.0	31.0	34.0
3	32.926542233653684	34.0	31.0	34.0	31.0	34.0
4	36.33339850425494	37.0	37.0	37.0	35.0	37.0
5	36.25548159862822	37.0	37.0	37.0	35.0	37.0
6	36.308552452454194	37.0	37.0	37.0	35.0	37.0
7	36.298820261494704	37.0	37.0	37.0	35.0	37.0
8	36.27766578034213	37.0	37.0	37.0	35.0	37.0
9	38.114916610185205	39.0	39.0	39.0	37.0	39.0
10	38.05569651784525	39.0	38.0	39.0	35.0	39.0
11	38.12731211947423	39.0	38.0	39.0	37.0	39.0
12	38.063686472833865	39.0	38.0	39.0	37.0	39.0
13	38.11549301078072	39.0	38.0	39.0	37.0	39.0
14	39.52508501184663	41.0	39.0	41.0	37.0	41.0
15	39.53546746377945	41.0	39.0	41.0	37.0	41.0
16	39.48163483429207	41.0	39.0	41.0	37.0	41.0
17	39.45416022198664	41.0	39.0	41.0	37.0	41.0
18	39.48093533306686	41.0	39.0	41.0	37.0	41.0
19	39.506476541364705	41.0	39.0	41.0	37.0	41.0
20	39.50877200604786	41.0	39.0	41.0	37.0	41.0
21	39.47067308527832	41.0	39.0	41.0	37.0	41.0
22	39.45187779148781	41.0	39.0	41.0	37.0	41.0
23	39.38881551125864	41.0	39.0	41.0	37.0	41.0
24	39.40236816646681	41.0	39.0	41.0	36.0	41.0
25	39.35603743417737	41.0	39.0	41.0	36.0	41.0
26	39.301948465731684	41.0	39.0	41.0	36.0	41.0
27	39.19270607045411	40.0	39.0	41.0	36.0	41.0
28	39.191369342439884	40.0	39.0	41.0	36.0	41.0
29	39.17374422875283	40.0	39.0	41.0	36.0	41.0
30	39.13304716057536	40.0	39.0	41.0	35.0	41.0
31	39.04252764695319	40.0	39.0	41.0	35.0	41.0
32	39.001073147842405	40.0	39.0	41.0	35.0	41.0
33	38.939060843572406	40.0	38.0	41.0	35.0	41.0
34	38.913038818697395	40.0	38.0	41.0	35.0	41.0
35	38.85108444413549	40.0	38.0	41.0	35.0	41.0
36	38.80223232130133	40.0	38.0	41.0	35.0	41.0
37	38.75918185873262	40.0	38.0	41.0	35.0	41.0
38	38.727547893386166	40.0	38.0	41.0	35.0	41.0
39	38.656607172856454	40.0	38.0	41.0	35.0	41.0
40	38.34453693887836	40.0	38.0	41.0	34.0	41.0
41	38.413523980002665	40.0	38.0	41.0	34.0	41.0
42	38.40335441974708	40.0	38.0	41.0	34.0	41.0
43	37.946774184204884	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.0
17	1.0
18	4.0
19	7.0
20	25.0
21	38.0
22	84.0
23	176.0
24	305.0
25	497.0
26	857.0
27	1315.0
28	2088.0
29	3111.0
30	4243.0
31	5979.0
32	7829.0
33	10808.0
34	15917.0
35	23914.0
36	37369.0
37	67669.0
38	154349.0
39	353904.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.208309437328744	16.833649050242435	13.453740231602973	27.504301280825842
2	19.73549295285101	20.08133331016145	33.904230606581976	26.278943130405562
3	20.49104117064354	20.48235171442971	30.20556356916517	28.821043545761572
4	15.047241676949191	16.629736477757888	34.596490618283774	33.72653122700915
5	16.086355815853043	33.45904079989341	34.199672117852195	16.25493126640135
6	32.59270201537455	34.578822057315655	16.300550911523956	16.527925015785847
7	29.31040475487044	28.851022169699288	20.771131309269332	21.06744176616094
8	26.73542923017211	31.570966788898353	20.731739107766636	20.961864873162906
9	28.233491481436424	13.376838544110575	18.522444865400324	39.867225109052676
10	18.7231713039398	24.527583230508103	30.24090069110142	26.50834477445068
11	37.59507713340632	20.333327540362525	19.680170081622958	22.391425244608193
12	22.075418686965236	24.93960827931388	27.288513118182394	25.696459915538483
13	34.07425429983258	18.3974615201914	21.915243044090303	25.613041135885716
14	23.460662831719993	21.013277489094733	24.26487200430997	31.261187674875305
15	28.71546665276354	25.144534621690042	21.08177936891376	25.058219356632662
16	24.548293101151064	25.065026097333497	23.55870886266604	26.827971938849398
17	25.913117023803316	24.734102639856797	22.735672534946097	26.617107801393793
18	25.776547736975953	22.31322013868372	24.456619338095155	27.45361278624517
19	27.108641374556115	23.40606408184309	24.65676647955371	24.828528064047084
20	29.011632285384913	21.641959646165343	24.100641281868583	25.245766786581164
21	26.29719098845461	23.47746244706673	23.1311876169456	27.094158947533064
22	27.279389189157875	23.78333130579355	22.718148798248205	26.21913070680037
23	26.59190837837368	23.184193299849962	23.70570549695	26.518192824826354
24	26.806103474044598	22.669632667720986	24.316139795971566	26.20812406226285
25	26.544985314818998	22.86355236555963	24.586671532762146	26.00479078685923
26	26.53615103433494	23.656754893612092	23.75407680320699	26.053017268845984
27	25.852725303117197	22.6331369516229	25.375239684167234	26.138898061092668
28	25.2573527281996	23.718160384189822	23.537854167752847	27.486632719857724
29	26.059534361006353	24.427075186968132	23.701505593113318	25.811884858912197
30	25.768872050653734	23.14393215272588	24.848513813338894	26.238681983281488
31	26.751794372708154	23.383326671416903	24.064435214310954	25.80044374156399
32	24.61331919848456	23.353058398938728	24.248506861773926	27.785115540802792
33	24.7578538201746	22.683680621933348	25.989004941404097	26.569460616487955
34	25.330633808936238	22.75174802894168	25.27762812603187	26.63999003609021
35	24.7032550702977	24.156977922988247	25.67459145073368	25.465175555980373
36	25.0868945621383	22.988246062228093	26.169890454921998	25.754968920711608
37	25.543235837634615	22.78274042277101	25.61318596015595	26.06083777943843
38	24.725558007913197	22.10814897203733	26.705305781964167	26.46098723808531
39	24.352635512069654	21.12797831111729	27.71806190368607	26.801324273126987
40	23.99100930930409	22.042109104812223	28.306338089362367	25.66054349652132
41	22.323357837599854	21.438771195031947	29.418733309002853	26.81913765836534
42	21.21733488584951	21.8606442942134	29.734015745294663	27.18800507464243
43	20.317396870637168	22.568110854289404	29.752263603343703	27.36222867172972
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11.0
1	14.0
2	17.0
3	36.0
4	55.0
5	55.0
6	82.5
7	110.0
8	109.5
9	109.0
10	220.0
11	331.0
12	331.0
13	637.5
14	944.0
15	1809.5
16	2675.0
17	2640.5
18	2606.0
19	2606.0
20	2916.5
21	3227.0
22	2879.5
23	2532.0
24	2785.0
25	3038.0
26	3038.0
27	3366.0
28	3694.0
29	5068.5
30	6443.0
31	7333.0
32	8223.0
33	8223.0
34	9819.5
35	11416.0
36	12659.5
37	13903.0
38	17233.5
39	20564.0
40	20564.0
41	24022.5
42	27481.0
43	30522.0
44	33563.0
45	44917.5
46	56272.0
47	56272.0
48	62054.0
49	67836.0
50	75861.0
51	83886.0
52	83355.5
53	82825.0
54	82825.0
55	72469.0
56	62113.0
57	56970.5
58	51828.0
59	47216.5
60	42605.0
61	42605.0
62	38179.5
63	33754.0
64	27906.5
65	22059.0
66	18851.0
67	15643.0
68	15643.0
69	13264.0
70	10885.0
71	9197.0
72	7509.0
73	6122.5
74	4736.0
75	4736.0
76	3885.0
77	3034.0
78	2468.5
79	1903.0
80	1524.0
81	1145.0
82	1145.0
83	920.0
84	695.0
85	548.5
86	402.0
87	324.0
88	246.0
89	246.0
90	189.5
91	133.0
92	82.0
93	31.0
94	20.0
95	9.0
96	9.0
97	4.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	690492.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.123243154114476
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.76334175406562	41.75333392094433
2	6.193747758309264	5.961264588167364
3	2.027124382295473	2.9265539865851746
4	1.0726982389760011	2.0648687274093
5	0.653555480903555	1.5725604661112997
6	0.45161400706641047	1.3039878404316507
7	0.35520264560494363	1.1965452298402017
8	0.2586449149407893	0.9957465705816687
9	0.22184054041398735	0.9608117641004225
>10	1.630232374667116	16.048751760507727
>50	0.2212042816938831	7.352658509332242
>100	0.13810157279706423	12.585319348316256
>500	0.00815917388516564	2.4098279092342163
>1k	0.004532874380647577	2.86776937843816
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1708	0.24735985355369794	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1630	0.23606356047571878	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1461	0.21158825880676388	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1424	0.2062297608082353	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1385	0.2005816142692457	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1359	0.19681618324325265	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1321	0.19131286097449357	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1310	0.18971979400195801	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1279	0.18523024162481247	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1255	0.1817544591392804	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1200	0.17378912427660276	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1122	0.1624928311986236	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1112	0.16104458849631856	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1106	0.16017564287493555	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1048	0.1517758352015664	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	840	0.12165238699362194	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	832	0.12049379283177793	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	819	0.11861107731878139	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	740	0.10716995997057172	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.448242702305023E-4	0.0	0.0	0.0	0.0
3	1.448242702305023E-4	0.0	0.0	0.0	0.0
4	1.448242702305023E-4	0.0	0.0	0.0	0.0
5	1.448242702305023E-4	0.0	0.0	0.0	0.0
6	1.448242702305023E-4	0.0	0.0	0.0	0.0
7	1.448242702305023E-4	0.0	0.0	0.0	0.0
8	2.896485404610046E-4	0.0	0.0	0.0	0.0
9	2.896485404610046E-4	0.0	0.0	0.0	0.0
10	2.896485404610046E-4	0.0	0.0	0.0	0.0
11	2.896485404610046E-4	0.0	0.0	0.0	0.0
12	2.896485404610046E-4	0.0	0.0	0.0	0.0
13	2.896485404610046E-4	0.0	0.0	0.0	0.0
14	4.344728106915069E-4	0.0	0.0	1.448242702305023E-4	0.0
15	7.241213511525116E-4	0.0	0.0	4.344728106915069E-4	0.0
16	7.241213511525116E-4	0.0	0.0	0.0010137698916135162	0.0
17	0.0010137698916135162	0.0	0.0	0.002172364053457535	0.0
18	0.0011585941618440184	0.0	0.0	0.0026068368641490413	0.0
19	0.0011585941618440184	0.0	0.0	0.0033309582153015532	0.0
20	0.0011585941618440184	0.0	0.0	0.005068849458067581	0.0
21	0.0011585941618440184	0.0	0.0	0.0073860377817556175	0.0
22	0.0011585941618440184	0.0	0.0	0.011151468807748678	0.0
23	0.0011585941618440184	0.0	0.0	0.013034184320745207	0.0
24	0.0011585941618440184	0.0	1.448242702305023E-4	0.015206548374202742	0.0
25	0.0011585941618440184	0.0	1.448242702305023E-4	0.017234088157429776	0.0
26	0.0011585941618440184	0.0	1.448242702305023E-4	0.020275397832270323	0.0
27	0.0011585941618440184	0.0	1.448242702305023E-4	0.03939220150269663	0.0
28	0.0011585941618440184	0.0	1.448242702305023E-4	0.1309211402883741	0.0
29	0.0013034184320745207	0.0	1.448242702305023E-4	0.3031171975924413	0.0
30	0.0013034184320745207	0.0	1.448242702305023E-4	0.5126779166159782	0.0
31	0.0013034184320745207	0.0	1.448242702305023E-4	1.1713386976243028	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1040	0.0	30.774038	1
TACGGCT	70	6.5829227E-9	26.428572	30
GTGTTGA	55	1.9012328E-5	23.545454	1
TAGCGAA	55	1.9012328E-5	23.545454	10
CTATATG	40	0.00193041	23.125002	1
ATTGGAG	80	6.951832E-7	20.8125	3
CTTATAC	930	0.0	19.892473	37
TAAGGTA	85	1.243834E-6	19.588236	5
ATACGGC	95	1.6736885E-7	19.473682	29
GTATCAA	1680	0.0	18.940475	2
GTAGCGA	70	1.2183026E-4	18.5	9
ATTAGAG	50	0.0070321085	18.499998	3
GGACGTA	50	0.0070321085	18.499998	27
TTACGAG	50	0.0070321085	18.499998	3
CAGTCGG	225	0.0	18.088888	10
GCAGTCG	230	0.0	17.695652	9
TAAAGAC	255	0.0	17.411764	37
TAGTTCC	85	2.7205655E-5	17.411764	4
ACGGTAC	160	3.45608E-11	17.34375	3
GTAGAAC	150	2.5102054E-10	17.266666	3
>>END_MODULE