FastQCFastQC Report
Fri 10 Feb 2017
ERR1631840.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631840.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398730
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9560.23976124194316956No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8640.21668798435031225No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA7700.19311313420108844No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7650.1918591528101723No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7390.18533844957740825No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7270.18232889423920948No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6590.16527474732274974No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6420.1610112105936348No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6060.15198254457903845No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5890.1477190078499235No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5730.14370626739899178No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5620.14094750833897624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5570.1396935269480601No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5230.1311664534898302No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4710.11812504702430215No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4620.11586788052065308No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA4560.1143631028515537No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA4040.10132169638602563No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3990.10006771499510947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6700.030.9253731
TATGGAG250.00549209229.65
TATAATA551.8986213E-523.5454542
CTATACC400.001928987423.1254
CAGTACT400.001928987423.1254
TCAGTAC400.001928987423.1253
CAGTCGG1350.021.92592610
GCAGTCG1450.021.6896559
CTTATAC5150.021.55339637
GGACCGT957.1104296E-921.4210536
CGGCCTT705.0881426E-621.14285724
ATCTCGC806.9366797E-720.812511
GTACCGG450.00382149220.5555556
TCTTAGG450.00382149220.5555552
CGTTAGA450.00382149220.5555552
GTATCAA10150.020.2315272
GGGCTAA555.135002E-420.1818181
GGCCTTT851.2411383E-619.58823425
ACCATGC951.6692684E-719.4736848
CTCTATG1900.019.4736841
Produced by FastQC (version 0.11.5)