##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631840.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	398730
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.639851528603316	34.0	31.0	34.0	31.0	34.0
2	32.80502595741479	34.0	31.0	34.0	31.0	34.0
3	32.893311263260856	34.0	31.0	34.0	31.0	34.0
4	36.30704988337973	37.0	37.0	37.0	35.0	37.0
5	36.22897700198129	37.0	35.0	37.0	35.0	37.0
6	36.28278032753994	37.0	37.0	37.0	35.0	37.0
7	36.26866802096657	37.0	37.0	37.0	35.0	37.0
8	36.25158127053395	37.0	37.0	37.0	35.0	37.0
9	38.08865146841221	39.0	38.0	39.0	37.0	39.0
10	38.02156346399819	39.0	38.0	39.0	35.0	39.0
11	38.08949915983247	39.0	38.0	39.0	37.0	39.0
12	38.030045394126354	39.0	38.0	39.0	35.0	39.0
13	38.088478418980266	39.0	38.0	39.0	37.0	39.0
14	39.48049557344569	41.0	39.0	41.0	37.0	41.0
15	39.48768339477842	41.0	39.0	41.0	37.0	41.0
16	39.4366789556843	41.0	39.0	41.0	37.0	41.0
17	39.40903618990294	41.0	39.0	41.0	37.0	41.0
18	39.43066486093346	41.0	39.0	41.0	37.0	41.0
19	39.45625862112206	41.0	39.0	41.0	37.0	41.0
20	39.44649762997517	41.0	39.0	41.0	37.0	41.0
21	39.41462142301808	41.0	39.0	41.0	37.0	41.0
22	39.386840719283725	40.0	39.0	41.0	37.0	41.0
23	39.32087377423319	40.0	39.0	41.0	36.0	41.0
24	39.342893687457675	41.0	39.0	41.0	36.0	41.0
25	39.289988212574926	40.0	39.0	41.0	36.0	41.0
26	39.23017079226544	40.0	39.0	41.0	36.0	41.0
27	39.1245027963785	40.0	39.0	41.0	36.0	41.0
28	39.11679331878715	40.0	39.0	41.0	36.0	41.0
29	39.09671958468137	40.0	39.0	41.0	35.0	41.0
30	39.06313545506985	40.0	39.0	41.0	35.0	41.0
31	38.97932937075214	40.0	39.0	41.0	35.0	41.0
32	38.936001805733206	40.0	38.0	41.0	35.0	41.0
33	38.86884107039852	40.0	38.0	41.0	35.0	41.0
34	38.84378401424523	40.0	38.0	41.0	35.0	41.0
35	38.78847591101748	40.0	38.0	41.0	35.0	41.0
36	38.74671080681163	40.0	38.0	41.0	35.0	41.0
37	38.69860055676774	40.0	38.0	41.0	35.0	41.0
38	38.6545557143932	40.0	38.0	41.0	35.0	41.0
39	38.58226870313244	40.0	38.0	41.0	35.0	41.0
40	38.28308379103654	40.0	38.0	41.0	34.0	41.0
41	38.358530835402405	40.0	38.0	41.0	34.0	41.0
42	38.34501291600833	40.0	38.0	41.0	34.0	41.0
43	37.877365635893966	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	2.0
18	2.0
19	7.0
20	10.0
21	29.0
22	71.0
23	123.0
24	170.0
25	308.0
26	542.0
27	819.0
28	1189.0
29	1822.0
30	2537.0
31	3561.0
32	4844.0
33	6683.0
34	9522.0
35	14389.0
36	22347.0
37	39936.0
38	92334.0
39	197480.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.540315501717956	16.60973591151907	13.155769568379604	27.69417901838337
2	20.27712988739247	19.729641612118478	33.385248162917264	26.60798033757179
3	20.776716073533468	20.16527474732275	29.70656835452562	29.351440824618162
4	15.404158202292278	16.213979384545933	33.90514884759111	34.47671356557069
5	16.598199282722646	33.266621523336596	33.60168535098939	16.533493842951373
6	33.49509693276152	34.01675319138264	15.848569207232966	16.639580668622877
7	29.85403656609736	28.368570210418078	20.346600456449227	21.430792767035335
8	27.12938579991473	31.443583377222684	20.30898101472174	21.11804980814085
9	28.315652195721412	13.271888245178442	18.1034785443784	40.30898101472174
10	18.99982444260527	24.12710355378326	29.88513530459208	26.98793669901939
11	38.26173099591202	20.147217415293557	19.28322423695232	22.3078273518421
12	22.529280465477893	24.254508063100346	26.922478870413563	26.2937326010082
13	34.26153035888947	18.125297820580343	21.487472725904748	26.12569909462544
14	23.630777719258646	20.768690592631607	23.545506984676347	32.055024703433396
15	29.162842023424375	24.806259875103454	20.512627592606524	25.518270508865648
16	25.129034685125273	24.63847716499887	22.908986030647306	27.32350211922855
17	26.411105259197953	24.328492965164397	22.294535149098387	26.965866626539263
18	26.06300002507963	22.095152107942717	23.97562260176059	27.866225265217064
19	27.735309608005416	22.945602287262055	24.00195621096983	25.317131893762696
20	29.24460161011211	21.30689940561282	23.616733127680387	25.83176585659469
21	26.765981992827225	22.995009154064153	22.573671406716326	27.665337446392297
22	27.661826298497733	23.386251348029997	22.191207082486898	26.76071527098538
23	27.098036265141822	22.913751159932787	23.164296641837833	26.823915933087555
24	27.38770596644346	22.288014445865624	23.619742683018586	26.70453690467233
25	27.267323752915505	22.60050660848193	23.72883906402829	26.403330574574273
26	27.25453314272816	23.292202743711282	22.915757530158253	26.537506583402305
27	26.57537682140797	22.301557444887518	24.660296441200813	26.4627692925037
28	25.60981115040253	23.402803902390087	23.015825245153362	27.971559702054023
29	26.68597798008678	24.012489654653525	22.989491635944123	26.312040729315576
30	26.207960273869535	22.957389712336667	24.180773957314475	26.65387605647932
31	27.268326938028242	22.910240012038223	23.590148722192964	26.23128432774058
32	25.073357911368593	22.982469340154992	23.671908308880695	28.272264439595716
33	25.43249818172698	22.410904622175405	25.08113259599228	27.075464600105338
34	25.76480325031977	22.462819451759337	24.6545281268026	27.1178491711183
35	25.311112783086298	23.700499084593584	24.901813257091266	26.08657487522885
36	25.57344569006596	22.621322699571138	25.50899104657287	26.296240563790036
37	26.051964988839565	22.156346399819427	25.14433325809445	26.64735535324656
38	25.32465578210819	21.59932786597447	26.253103603942517	26.82291274797482
39	24.607629222782336	20.717528151882224	27.44313194392195	27.23171068141349
40	24.433075013166807	21.678328693602186	27.937451408221104	25.951144885009906
41	22.867855441025256	20.875028214581295	28.99505931331979	27.26205703107366
42	21.506784039324856	21.33875053294209	29.534522107691924	27.61994332004113
43	20.680159506432926	22.127254031550173	29.56511925363028	27.62746720838663
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	2.5
2	4.0
3	11.5
4	19.0
5	19.0
6	27.5
7	36.0
8	38.5
9	41.0
10	78.5
11	116.0
12	116.0
13	251.5
14	387.0
15	744.0
16	1101.0
17	1078.0
18	1055.0
19	1055.0
20	1157.5
21	1260.0
22	1141.0
23	1022.0
24	1092.0
25	1162.0
26	1162.0
27	1343.0
28	1524.0
29	2149.0
30	2774.0
31	3197.5
32	3621.0
33	3621.0
34	4531.5
35	5442.0
36	6095.5
37	6749.0
38	8877.0
39	11005.0
40	11005.0
41	13018.0
42	15031.0
43	16752.5
44	18474.0
45	24958.0
46	31442.0
47	31442.0
48	35018.5
49	38595.0
50	43209.5
51	47824.0
52	48286.5
53	48749.0
54	48749.0
55	43159.5
56	37570.0
57	34710.0
58	31850.0
59	29052.5
60	26255.0
61	26255.0
62	23824.0
63	21393.0
64	17624.0
65	13855.0
66	12052.5
67	10250.0
68	10250.0
69	8671.5
70	7093.0
71	6084.5
72	5076.0
73	4068.5
74	3061.0
75	3061.0
76	2479.5
77	1898.0
78	1571.0
79	1244.0
80	1008.0
81	772.0
82	772.0
83	608.5
84	445.0
85	365.0
86	285.0
87	215.5
88	146.0
89	146.0
90	114.5
91	83.0
92	49.5
93	16.0
94	10.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	398730.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.543727801410945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.0713279150128	43.5036576964239
2	6.384572184882585	6.454001572823298
3	2.1925756456066297	3.3246283984663303
4	1.1476340117181936	2.3202280441570253
5	0.7124513438219149	1.8004973396942154
6	0.4809370498045298	1.4585010800960263
7	0.38987600699464714	1.3794050741686905
8	0.2771018275235507	1.1204607474899095
9	0.2433499862489634	1.1069833918900231
>10	1.7764083454585209	17.97108383142311
>50	0.20466795103156163	7.109755225375454
>100	0.11209198296105029	10.039905146612508
>500	0.007005748935065643	2.4108924513795293
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	956	0.23976124194316956	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	864	0.21668798435031225	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	770	0.19311313420108844	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	765	0.1918591528101723	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	739	0.18533844957740825	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	727	0.18232889423920948	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	659	0.16527474732274974	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	642	0.1610112105936348	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	606	0.15198254457903845	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	589	0.1477190078499235	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	573	0.14370626739899178	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	562	0.14094750833897624	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	557	0.1396935269480601	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	523	0.1311664534898302	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	471	0.11812504702430215	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	462	0.11586788052065308	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	456	0.1143631028515537	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	404	0.10132169638602563	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	399	0.10006771499510947	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.507962781832318E-4	0.0
5	0.0	0.0	0.0	2.507962781832318E-4	0.0
6	0.0	0.0	0.0	2.507962781832318E-4	2.507962781832318E-4
7	0.0	0.0	0.0	2.507962781832318E-4	2.507962781832318E-4
8	5.015925563664636E-4	0.0	0.0	2.507962781832318E-4	2.507962781832318E-4
9	5.015925563664636E-4	0.0	0.0	5.015925563664636E-4	2.507962781832318E-4
10	5.015925563664636E-4	0.0	0.0	5.015925563664636E-4	2.507962781832318E-4
11	5.015925563664636E-4	0.0	0.0	7.523888345496953E-4	2.507962781832318E-4
12	5.015925563664636E-4	0.0	0.0	7.523888345496953E-4	2.507962781832318E-4
13	5.015925563664636E-4	0.0	0.0	7.523888345496953E-4	2.507962781832318E-4
14	5.015925563664636E-4	0.0	0.0	7.523888345496953E-4	2.507962781832318E-4
15	5.015925563664636E-4	0.0	0.0	0.0010031851127329271	2.507962781832318E-4
16	5.015925563664636E-4	0.0	0.0	0.0017555739472826224	2.507962781832318E-4
17	5.015925563664636E-4	0.0	0.0	0.0022571665036490857	2.507962781832318E-4
18	5.015925563664636E-4	0.0	0.0	0.0025079627818323175	2.507962781832318E-4
19	5.015925563664636E-4	0.0	0.0	0.003009555338198781	2.507962781832318E-4
20	7.523888345496953E-4	0.0	0.0	0.004514333007298171	2.507962781832318E-4
21	7.523888345496953E-4	0.0	0.0	0.008777869736413112	2.507962781832318E-4
22	7.523888345496953E-4	0.0	0.0	0.013793795300077747	2.507962781832318E-4
23	7.523888345496953E-4	0.0	0.0	0.016301758081910065	2.507962781832318E-4
24	7.523888345496953E-4	0.0	0.0	0.020816091089208236	2.507962781832318E-4
25	7.523888345496953E-4	0.0	0.0	0.02282246131467409	2.507962781832318E-4
26	7.523888345496953E-4	0.0	0.0	0.02859077571288842	2.507962781832318E-4
27	7.523888345496953E-4	0.0	0.0	0.0481528854111805	2.507962781832318E-4
28	7.523888345496953E-4	0.0	0.0	0.1419506934517092	2.507962781832318E-4
29	7.523888345496953E-4	0.0	0.0	0.3079778296090086	2.507962781832318E-4
30	7.523888345496953E-4	0.0	0.0	0.5010909638100971	2.507962781832318E-4
31	7.523888345496953E-4	0.0	0.0	1.144885009906453	2.507962781832318E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	670	0.0	30.925373	1
TATGGAG	25	0.005492092	29.6	5
TATAATA	55	1.8986213E-5	23.545454	2
CTATACC	40	0.0019289874	23.125	4
CAGTACT	40	0.0019289874	23.125	4
TCAGTAC	40	0.0019289874	23.125	3
CAGTCGG	135	0.0	21.925926	10
GCAGTCG	145	0.0	21.689655	9
CTTATAC	515	0.0	21.553396	37
GGACCGT	95	7.1104296E-9	21.421053	6
CGGCCTT	70	5.0881426E-6	21.142857	24
ATCTCGC	80	6.9366797E-7	20.8125	11
GTACCGG	45	0.003821492	20.555555	6
TCTTAGG	45	0.003821492	20.555555	2
CGTTAGA	45	0.003821492	20.555555	2
GTATCAA	1015	0.0	20.231527	2
GGGCTAA	55	5.135002E-4	20.181818	1
GGCCTTT	85	1.2411383E-6	19.588234	25
ACCATGC	95	1.6692684E-7	19.473684	8
CTCTATG	190	0.0	19.473684	1
>>END_MODULE