FastQCFastQC Report
Fri 10 Feb 2017
ERR1631839.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631839.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60351
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1520.25185995261056154No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1510.2502029792381236No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1430.23694719225862043No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1360.22534837865155508No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1350.22369140527911716No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1240.2054646981823002No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1190.1971798313201107No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1050.17398220410598No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1010.1673543106162284No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT930.15409852363672516No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA870.14415668340209772No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA860.14249971002965983No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT820.1358718165399082No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT810.1342148431674703No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA800.1325578697950324No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT760.12592997630528077No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC730.12095905618796705No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC710.11764510944309126No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA680.11267418932577754No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT670.11101721595333962No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA660.10936024258090174No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC650.10770326920846382No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA650.10770326920846382No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA640.1060462958360259No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACACA251.2198194E-436.99999637
TATACAC303.5595716E-430.83333436
GGTATCA652.8721843E-928.4615381
CTATGGG358.771989E-426.428573
CTCTATG400.001910179223.1251
TCTATGG400.001910179223.1252
TTATACA400.001910179223.12535
TATGGGC400.001910179223.1254
GTATCAA1400.022.4642851
CTTATAC701.1950923E-418.537
CGCTTGC500.00695980718.4999981
CCTCCGT500.00695980718.49999810
CCCTCCG500.00695980718.4999989
CTTCTGG500.00695980718.4999981
TACGGGC650.001556610217.07692315
TCAACGC1951.8189894E-1216.1282064
TATCAAC1951.8189894E-1216.1282062
ATCAACG2003.6379788E-1215.7249993
CAACGCA2003.6379788E-1215.7249995
AACGCAG2055.456968E-1215.3414636
Produced by FastQC (version 0.11.5)