##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631839.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	60351
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.619724611025504	34.0	31.0	34.0	31.0	34.0
2	32.78858676741065	34.0	31.0	34.0	31.0	34.0
3	32.87491508011466	34.0	31.0	34.0	31.0	34.0
4	36.303971765173735	37.0	37.0	37.0	35.0	37.0
5	36.2208248413448	37.0	35.0	37.0	35.0	37.0
6	36.25661546618946	37.0	37.0	37.0	35.0	37.0
7	36.249258504415835	37.0	37.0	37.0	35.0	37.0
8	36.222249838445094	37.0	37.0	37.0	35.0	37.0
9	38.0534871004623	39.0	38.0	39.0	35.0	39.0
10	37.98387764908618	39.0	38.0	39.0	35.0	39.0
11	38.07018939205647	39.0	38.0	39.0	37.0	39.0
12	37.99166542393664	39.0	38.0	39.0	35.0	39.0
13	38.049841759042934	39.0	38.0	39.0	35.0	39.0
14	39.46640486487382	41.0	39.0	41.0	37.0	41.0
15	39.454557505260894	41.0	39.0	41.0	37.0	41.0
16	39.40151778760915	40.0	39.0	41.0	37.0	41.0
17	39.36806349521963	40.0	39.0	41.0	37.0	41.0
18	39.39371344302497	40.0	39.0	41.0	37.0	41.0
19	39.40574306970887	41.0	39.0	41.0	37.0	41.0
20	39.41812066080098	41.0	39.0	41.0	37.0	41.0
21	39.38242945435867	40.0	39.0	41.0	37.0	41.0
22	39.3609053702507	40.0	39.0	41.0	36.0	41.0
23	39.2869546486388	40.0	39.0	41.0	36.0	41.0
24	39.30226508260012	40.0	39.0	41.0	36.0	41.0
25	39.25408029692963	40.0	39.0	41.0	36.0	41.0
26	39.17285546221272	40.0	39.0	41.0	36.0	41.0
27	39.07080247220427	40.0	39.0	41.0	36.0	41.0
28	39.09240940498086	40.0	39.0	41.0	35.0	41.0
29	39.04304816821594	40.0	39.0	41.0	35.0	41.0
30	39.02223658265812	40.0	39.0	41.0	35.0	41.0
31	38.92503852463091	40.0	38.0	41.0	35.0	41.0
32	38.89234643999271	40.0	38.0	41.0	35.0	41.0
33	38.82068234163477	40.0	38.0	41.0	35.0	41.0
34	38.80946463190337	40.0	38.0	41.0	35.0	41.0
35	38.73940779771669	40.0	38.0	41.0	35.0	41.0
36	38.70782588523802	40.0	38.0	41.0	35.0	41.0
37	38.65793441699392	40.0	38.0	41.0	35.0	41.0
38	38.60366853904658	40.0	38.0	41.0	35.0	41.0
39	38.53049659491972	40.0	38.0	41.0	35.0	41.0
40	38.19283856108432	40.0	38.0	41.0	34.0	41.0
41	38.29972991334029	40.0	37.0	41.0	34.0	41.0
42	38.31671389040778	40.0	37.0	41.0	34.0	41.0
43	37.85219797517854	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	0.0
19	1.0
20	5.0
21	6.0
22	5.0
23	10.0
24	34.0
25	44.0
26	82.0
27	126.0
28	226.0
29	276.0
30	417.0
31	533.0
32	746.0
33	1054.0
34	1479.0
35	2282.0
36	3467.0
37	6032.0
38	14230.0
39	29295.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.454971748603995	16.531623336812977	12.627794071349275	28.38561084323375
2	21.048532750078706	19.514175407201208	31.633278653212045	27.80401318950804
3	20.71879504896356	19.777634173418836	29.131248860830805	30.3723219167868
4	15.563950887309241	15.699822703849147	33.482460936852746	35.253765471988864
5	16.907756292356382	33.17592086295173	33.459263309638615	16.457059535053272
6	34.21318619409786	33.75420456993256	15.428079070769332	16.604530165200245
7	30.715315404881444	27.494159168862158	19.771006279929082	22.01951914632732
8	27.63665887889182	30.547961094265215	20.60114993952047	21.2142300873225
9	28.03598946164935	12.682474192639726	17.47775513247502	41.8037812132359
10	18.960746300806946	23.563818329439446	29.629997845934614	27.845437523818994
11	39.540355586485724	19.323623469370848	18.49845072989677	22.637570214246658
12	22.939139368030357	23.635068184454276	26.243144272671536	27.18264817484383
13	35.397922155390965	17.288860168017102	20.604463886265346	26.70875379032659
14	24.28791569319481	19.72461102550082	22.26972212556544	33.71775115573893
15	29.465957482063267	24.31939818727113	19.706384318404	26.5082600122616
16	26.16195257742208	24.045997580818877	21.593676989610778	28.198372852148267
17	27.426223260592202	23.69637619923448	20.955742241222183	27.921658298951137
18	26.56459710692449	20.98391078855363	23.23573760169674	29.215754502825142
19	28.577819754436547	22.092425974714587	22.993819489320806	26.33593478152806
20	30.216566419777635	20.698911368494308	22.34262895395271	26.741893257775345
21	27.67145531971301	21.96318205166443	21.340160063627778	29.02520256499478
22	29.048400192208913	22.425477622574604	20.8646087057381	27.661513479478383
23	28.112210236781493	22.481814717237494	21.653328031018543	27.752647014962466
24	28.417093337310067	21.255654421633444	22.46690195688555	27.86035028417093
25	28.62918592898212	21.916786797236167	22.253152391841063	27.200874881940646
26	28.151977597720006	22.743616510082685	21.769316167089194	27.33508972510812
27	27.205845802057958	21.36004374409703	23.84384682938145	27.590263624463557
28	26.476777518185283	22.727046776358304	21.653328031018543	29.14284767443787
29	27.613461251677684	23.429603486271976	21.930042584215673	27.026892677834667
30	26.851253500356247	21.868734569435468	23.30533048333913	27.97468144686915
31	27.938228032675518	22.56466338585939	22.549750625507446	26.947357955957646
32	25.146227900117644	22.632599294129342	22.621000480522277	29.600172325230734
33	26.23154545906447	21.724577886033373	24.183526370731222	27.86035028417093
34	26.947357955957646	21.698066312074367	23.36995244486421	27.984623287103776
35	26.110586402876507	23.398120992195658	24.01782903348743	26.47346357144041
36	26.430382263757025	22.117280575301155	24.196782157710725	27.2555550032311
37	27.19093304170602	22.140478202515286	23.79745157495319	26.871137180825507
38	26.191778098125962	21.18109061987374	25.447797053901343	27.179334228098956
39	25.777534755016486	20.34928998690991	26.13047008334576	27.742705174727845
40	25.15948368709715	21.596990936355652	26.94404400921277	26.299481367334426
41	23.161173799937036	20.490132723567132	28.17020430481682	28.17848917167901
42	22.223326871137182	20.803300690957897	29.144504647810308	27.828867790094613
43	21.411409918642608	21.56882238902421	28.736889198190585	28.2828784941426
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	2.5
7	4.0
8	3.5
9	3.0
10	4.5
11	6.0
12	6.0
13	12.0
14	18.0
15	39.0
16	60.0
17	57.5
18	55.0
19	55.0
20	68.5
21	82.0
22	63.0
23	44.0
24	63.0
25	82.0
26	82.0
27	104.5
28	127.0
29	177.5
30	228.0
31	284.5
32	341.0
33	341.0
34	457.0
35	573.0
36	651.0
37	729.0
38	1029.5
39	1330.0
40	1330.0
41	1582.5
42	1835.0
43	2131.5
44	2428.0
45	3440.0
46	4452.0
47	4452.0
48	4925.5
49	5399.0
50	6284.5
51	7170.0
52	7434.5
53	7699.0
54	7699.0
55	6820.5
56	5942.0
57	5669.5
58	5397.0
59	4947.5
60	4498.0
61	4498.0
62	4139.5
63	3781.0
64	3151.0
65	2521.0
66	2198.0
67	1875.0
68	1875.0
69	1594.5
70	1314.0
71	1117.0
72	920.0
73	732.5
74	545.0
75	545.0
76	453.5
77	362.0
78	291.5
79	221.0
80	178.5
81	136.0
82	136.0
83	111.5
84	87.0
85	64.5
86	42.0
87	31.5
88	21.0
89	21.0
90	17.5
91	14.0
92	10.5
93	7.0
94	4.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	60351.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.31707842454972
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.53132728771641	55.65442163344435
2	6.025865622423742	7.751321436264519
3	2.4294105523495464	4.687577670626833
4	1.3499587798845838	3.473016188629849
5	0.7599958779884584	2.4440357243459094
6	0.5642003297609234	2.177263011383407
7	0.4225061830173124	1.902205431558715
8	0.31687963726298435	1.6304617984788983
9	0.2550494641384996	1.4763632748421733
>10	1.2675185490519374	14.462063594638034
>50	0.05410140148392415	2.4092392835247134
>100	0.023186314921681783	1.9320309522625971
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	152	0.25185995261056154	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	151	0.2502029792381236	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	143	0.23694719225862043	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	136	0.22534837865155508	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	135	0.22369140527911716	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	124	0.2054646981823002	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	119	0.1971798313201107	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	105	0.17398220410598	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	101	0.1673543106162284	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	93	0.15409852363672516	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	87	0.14415668340209772	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	86	0.14249971002965983	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	82	0.1358718165399082	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	81	0.1342148431674703	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	80	0.1325578697950324	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	76	0.12592997630528077	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	73	0.12095905618796705	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	71	0.11764510944309126	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	68	0.11267418932577754	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	67	0.11101721595333962	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	66	0.10936024258090174	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	65	0.10770326920846382	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	65	0.10770326920846382	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	64	0.1060462958360259	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.001656973372437905	0.0	0.0	0.0	0.0
10	0.001656973372437905	0.0	0.0	0.0	0.0
11	0.001656973372437905	0.0	0.0	0.0	0.0
12	0.001656973372437905	0.0	0.0	0.0	0.0
13	0.001656973372437905	0.0	0.0	0.0	0.0
14	0.001656973372437905	0.0	0.0	0.0	0.0
15	0.001656973372437905	0.0	0.0	0.001656973372437905	0.0
16	0.001656973372437905	0.0	0.0	0.001656973372437905	0.0
17	0.001656973372437905	0.0	0.0	0.001656973372437905	0.0
18	0.001656973372437905	0.0	0.0	0.001656973372437905	0.0
19	0.001656973372437905	0.0	0.0	0.001656973372437905	0.0
20	0.001656973372437905	0.0	0.0	0.00331394674487581	0.0
21	0.001656973372437905	0.0	0.0	0.0049709201173137145	0.0
22	0.001656973372437905	0.0	0.0	0.0049709201173137145	0.0
23	0.001656973372437905	0.0	0.0	0.0049709201173137145	0.0
24	0.001656973372437905	0.0	0.0	0.0049709201173137145	0.0
25	0.001656973372437905	0.0	0.0	0.0049709201173137145	0.0
26	0.001656973372437905	0.0	0.0	0.014912760351941143	0.0
27	0.001656973372437905	0.0	0.0	0.02651157395900648	0.0
28	0.00331394674487581	0.0	0.0	0.11930208281552915	0.0
29	0.00331394674487581	0.0	0.0	0.26345876621762687	0.0
30	0.00331394674487581	0.0	0.0	0.41424334310947625	0.0
31	0.00331394674487581	0.0	0.0	0.9179632483305993	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACACA	25	1.2198194E-4	36.999996	37
TATACAC	30	3.5595716E-4	30.833334	36
GGTATCA	65	2.8721843E-9	28.461538	1
CTATGGG	35	8.771989E-4	26.42857	3
CTCTATG	40	0.0019101792	23.125	1
TCTATGG	40	0.0019101792	23.125	2
TTATACA	40	0.0019101792	23.125	35
TATGGGC	40	0.0019101792	23.125	4
GTATCAA	140	0.0	22.464285	1
CTTATAC	70	1.1950923E-4	18.5	37
CGCTTGC	50	0.006959807	18.499998	1
CCTCCGT	50	0.006959807	18.499998	10
CCCTCCG	50	0.006959807	18.499998	9
CTTCTGG	50	0.006959807	18.499998	1
TACGGGC	65	0.0015566102	17.076923	15
TCAACGC	195	1.8189894E-12	16.128206	4
TATCAAC	195	1.8189894E-12	16.128206	2
ATCAACG	200	3.6379788E-12	15.724999	3
CAACGCA	200	3.6379788E-12	15.724999	5
AACGCAG	205	5.456968E-12	15.341463	6
>>END_MODULE