FastQCFastQC Report
Fri 10 Feb 2017
ERR1631838.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631838.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658421
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC19180.2913029809195029No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT13870.21065549245847262No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG9040.1372981724458971No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8980.13638690138984025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8280.1257554057358438No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG8200.12454037766110133No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC8050.12226220002095924No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8020.12180656449293083No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA8020.12180656449293083No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT8000.1215028074742452No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT7680.1166426951752754No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA7420.11269385393236242No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT7250.11011191927353473No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG7240.1099600407641919No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC7190.10920064821747788No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC6940.1054036854839077No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT6830.10373302188113685No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6770.10282175082508001No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6700.10175860125968036No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6680.10145484424099475No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTG250.005494659829.59999837
GGTATCA6500.026.7538451
TATCCTA751.371518E-824.66666618
TATTAGA1150.024.1304362
GTACTAT551.9010582E-523.5454541
AGTTCCG400.001930316223.12532
CGGAATG652.6799553E-622.7692314
ATTAGAG1250.022.1999993
ATATAGC502.7004041E-422.1999996
TCTATGG2550.021.0392152
GCGGTAA3350.020.98507523
GACAGGC5150.020.8349517
CTTATAC5800.020.73275837
CTAAGCC450.003824097820.5555574
ATAGCAA555.139886E-420.1818188
TAGCAAG555.139886E-420.1818189
GCTCTAG555.139886E-420.1818181
GTATTAG2300.020.1086961
CATCAGA5100.019.95098116
ACTATAT656.896406E-519.9230773
Produced by FastQC (version 0.11.5)