##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631838.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	658421
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.646051690331866	34.0	31.0	34.0	31.0	34.0
2	32.81259710732191	34.0	31.0	34.0	31.0	34.0
3	32.90298608337219	34.0	31.0	34.0	31.0	34.0
4	36.3248818005501	37.0	37.0	37.0	35.0	37.0
5	36.24131824470969	37.0	37.0	37.0	35.0	37.0
6	36.286436793480156	37.0	37.0	37.0	35.0	37.0
7	36.26887963780013	37.0	37.0	37.0	35.0	37.0
8	36.24426772536113	37.0	37.0	37.0	35.0	37.0
9	38.0909615580305	39.0	38.0	39.0	37.0	39.0
10	38.02261774761133	39.0	38.0	39.0	35.0	39.0
11	38.100722789825966	39.0	38.0	39.0	37.0	39.0
12	38.04707930032608	39.0	38.0	39.0	35.0	39.0
13	38.084906161863	39.0	38.0	39.0	37.0	39.0
14	39.50640851370172	41.0	39.0	41.0	37.0	41.0
15	39.50314920089122	41.0	39.0	41.0	37.0	41.0
16	39.469596200607214	41.0	39.0	41.0	37.0	41.0
17	39.42270522963271	41.0	39.0	41.0	37.0	41.0
18	39.412281807536516	41.0	39.0	41.0	37.0	41.0
19	39.4381451988925	41.0	39.0	41.0	37.0	41.0
20	39.410889081605845	41.0	39.0	41.0	37.0	41.0
21	39.393615938738286	41.0	39.0	41.0	36.0	41.0
22	39.36619123630626	40.0	39.0	41.0	36.0	41.0
23	39.30875078407281	40.0	39.0	41.0	36.0	41.0
24	39.323795869208304	41.0	39.0	41.0	36.0	41.0
25	39.26140417757028	40.0	39.0	41.0	36.0	41.0
26	39.2002259952219	40.0	39.0	41.0	36.0	41.0
27	39.11699505331695	40.0	39.0	41.0	35.0	41.0
28	39.092527425461824	40.0	39.0	41.0	35.0	41.0
29	39.06964540924424	40.0	39.0	41.0	35.0	41.0
30	39.029977780174086	40.0	39.0	41.0	35.0	41.0
31	38.9594879264179	40.0	38.0	41.0	35.0	41.0
32	38.92376610102047	40.0	38.0	41.0	35.0	41.0
33	38.8704901575132	40.0	38.0	41.0	35.0	41.0
34	38.825948747078236	40.0	38.0	41.0	35.0	41.0
35	38.744160651012045	40.0	38.0	41.0	35.0	41.0
36	38.7176168439342	40.0	38.0	41.0	35.0	41.0
37	38.67887871134123	40.0	38.0	41.0	35.0	41.0
38	38.64940364903307	40.0	38.0	41.0	35.0	41.0
39	38.588506441926974	40.0	38.0	41.0	35.0	41.0
40	38.25104302566291	40.0	38.0	41.0	34.0	41.0
41	38.327896588960556	40.0	38.0	41.0	34.0	41.0
42	38.31893423812424	40.0	37.0	41.0	34.0	41.0
43	37.844405023533575	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	5.0
19	8.0
20	19.0
21	44.0
22	70.0
23	149.0
24	277.0
25	481.0
26	755.0
27	1268.0
28	2041.0
29	3039.0
30	4149.0
31	5827.0
32	7969.0
33	10627.0
34	16168.0
35	25869.0
36	38267.0
37	66394.0
38	150054.0
39	324938.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.587613396292035	16.017715109329743	11.883582085018551	28.511089409359663
2	20.268642707325558	18.123055005839728	33.97400751191107	27.634294774923646
3	20.782599582941614	19.906564341052306	28.53523809234517	30.77559798366091
4	15.33714750896463	14.717635069355323	33.30483079974667	36.64038662193339
5	16.947970979054436	33.309235276517605	32.98482278056137	16.757970963866583
6	36.198420159745815	33.28873167775633	14.597043532937132	15.915804629560723
7	31.772680397496433	27.536940650434904	19.6649256326879	21.02545331938076
8	28.50106542774304	31.64965880492876	18.904925571936495	20.944350195391703
9	28.877268495385174	12.87975322779802	16.937339483400436	41.30563879341637
10	19.246348460939124	23.549522266148863	29.795070327343755	27.40905894556826
11	39.109931183847415	19.599617873670493	18.96522134014559	22.3252296023365
12	24.73447839604144	23.05956219500897	26.032735893903748	26.173223515045844
13	33.050434296597466	17.601352326247188	21.529082456361508	27.81913092079384
14	24.042064271947584	18.834453943601435	23.440321617931385	33.6831601665196
15	28.943943160986663	23.633359203306092	20.72200005771383	26.70069757799341
16	26.349402585883503	23.58035360354545	21.30749171123035	28.762752099340695
17	26.47455047758197	23.85358304185316	22.251112889777207	27.420753590787655
18	26.458451355591635	21.681416601232343	23.82973811588634	28.030393927289683
19	28.158427510665668	21.891008944125414	22.877763619325627	27.072799925883288
20	28.112408322334797	21.575101644692378	23.390201709848256	26.922288323124565
21	28.510481895322293	22.09589305322886	22.052303921047475	27.34132113040137
22	28.796621006924138	23.045133736621402	21.228666764881435	26.929578491573018
23	28.05089752605096	22.791040990490888	22.009626059922148	27.148435423536004
24	28.382600190455648	22.103183221677316	21.98441422737124	27.529802360495793
25	27.507172462603712	22.018586891973374	23.22905861143554	27.245182033987376
26	28.251377158383466	22.424406268937354	22.910113741815646	26.414102830863534
27	28.316077403363497	21.86245578436897	22.712519801160656	27.108947011106878
28	26.443111626148013	22.13583710118602	23.09570928023256	28.32534199243341
29	27.84343148228869	22.496548560875183	22.991824379842075	26.66819557699405
30	26.8149102170192	21.69371876048911	24.048139412321294	27.443231610170393
31	26.942032529339134	22.3674518279338	23.136868356264458	27.553647286462613
32	25.355205863725487	22.218155253249822	23.538435134966836	28.888203748057855
33	26.424430569498846	22.480449438884847	24.1240786669927	26.971041324623606
34	26.86229631193416	22.2830073767392	23.966884409822896	26.88781190150375
35	26.805797506458635	22.892495834731882	24.085501525619627	26.216205133189856
36	26.783167608566554	22.041976182412164	23.754710132270993	27.420146076750285
37	25.759658334105385	21.9757571523387	24.322735757213092	27.941848756342825
38	26.446756710372238	20.893167137743177	25.323159498254157	27.33691665363043
39	24.780345705862967	20.744629955605912	26.394966138686343	28.080058199844782
40	25.052967630133306	20.921872176008968	27.29196061486496	26.73319957899277
41	23.53539756477998	20.234773799742108	28.264286831677605	27.965541803800303
42	22.466628494534653	21.364446152233903	28.31000226298979	27.858923090241657
43	22.286348703944743	20.352935280010815	28.928907188561727	28.43180882748272
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	10.0
2	11.0
3	23.5
4	36.0
5	36.0
6	51.5
7	67.0
8	69.0
9	71.0
10	109.0
11	147.0
12	147.0
13	287.0
14	427.0
15	888.0
16	1349.0
17	1345.5
18	1342.0
19	1342.0
20	1437.5
21	1533.0
22	1308.5
23	1084.0
24	1273.0
25	1462.0
26	1462.0
27	1816.0
28	2170.0
29	2946.0
30	3722.0
31	4894.5
32	6067.0
33	6067.0
34	8308.5
35	10550.0
36	12527.0
37	14504.0
38	17515.5
39	20527.0
40	20527.0
41	23285.0
42	26043.0
43	30571.5
44	35100.0
45	39044.5
46	42989.0
47	42989.0
48	46215.5
49	49442.0
50	55920.0
51	62398.0
52	64626.5
53	66855.0
54	66855.0
55	64313.5
56	61772.0
57	57086.5
58	52401.0
59	48793.5
60	45186.0
61	45186.0
62	43526.5
63	41867.0
64	34142.0
65	26417.0
66	23401.0
67	20385.0
68	20385.0
69	17782.5
70	15180.0
71	14118.5
72	13057.0
73	14341.0
74	15625.0
75	15625.0
76	13484.5
77	11344.0
78	7597.0
79	3850.0
80	2637.0
81	1424.0
82	1424.0
83	1173.5
84	923.0
85	736.5
86	550.0
87	425.0
88	300.0
89	300.0
90	233.0
91	166.0
92	113.5
93	61.0
94	33.5
95	6.0
96	6.0
97	3.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	658421.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.83411951194904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.3129695613172	40.42391931865949
2	6.271414876625238	5.874323876817631
3	2.186254893448531	3.0717396889005584
4	1.1643748412140096	2.1812988188049443
5	0.6812664490818051	1.5953257147889204
6	0.49063528561004927	1.3787082961824182
7	0.350900931994669	1.150389533012484
8	0.2794762545769274	1.047121944608617
9	0.22146609648425317	0.9334952667529515
>10	1.6446465229744966	15.398206252099328
>50	0.20513497677783096	6.826074264172539
>100	0.18038853347249365	16.33594451373438
>500	0.010419554279999629	3.2794496678384446
>1k	6.512221424999768E-4	0.5040028436273453
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1918	0.2913029809195029	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	1387	0.21065549245847262	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	904	0.1372981724458971	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	898	0.13638690138984025	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	828	0.1257554057358438	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	820	0.12454037766110133	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	805	0.12226220002095924	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	802	0.12180656449293083	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	802	0.12180656449293083	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	800	0.1215028074742452	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	768	0.1166426951752754	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	742	0.11269385393236242	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	725	0.11011191927353473	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	724	0.1099600407641919	No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC	719	0.10920064821747788	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	694	0.1054036854839077	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	683	0.10373302188113685	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	677	0.10282175082508001	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	670	0.10175860125968036	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	668	0.10145484424099475	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	1.518785093428065E-4
4	0.0	0.0	0.0	0.0	1.518785093428065E-4
5	0.0	0.0	0.0	0.0	1.518785093428065E-4
6	0.0	0.0	0.0	0.0	1.518785093428065E-4
7	0.0	0.0	0.0	0.0	1.518785093428065E-4
8	1.518785093428065E-4	0.0	0.0	0.0	1.518785093428065E-4
9	1.518785093428065E-4	0.0	0.0	0.0	1.518785093428065E-4
10	4.556355280284195E-4	0.0	0.0	0.0	1.518785093428065E-4
11	4.556355280284195E-4	0.0	0.0	0.0	1.518785093428065E-4
12	4.556355280284195E-4	0.0	0.0	1.518785093428065E-4	3.03757018685613E-4
13	4.556355280284195E-4	0.0	0.0	1.518785093428065E-4	3.03757018685613E-4
14	6.07514037371226E-4	0.0	0.0	3.03757018685613E-4	3.03757018685613E-4
15	9.11271056056839E-4	0.0	0.0	7.593925467140326E-4	3.03757018685613E-4
16	9.11271056056839E-4	0.0	0.0	0.0010631495653996455	3.03757018685613E-4
17	9.11271056056839E-4	0.0	0.0	0.002733813168170517	3.03757018685613E-4
18	0.001215028074742452	0.0	0.0	0.0028856916775133233	3.03757018685613E-4
19	0.0013669065840852584	0.0	0.0	0.003948841242912969	3.03757018685613E-4
20	0.0013669065840852584	0.0	0.0	0.004708233789627002	3.03757018685613E-4
21	0.0013669065840852584	0.0	0.0	0.007745803976483131	3.03757018685613E-4
22	0.0013669065840852584	0.0	0.0	0.010631495653996455	3.03757018685613E-4
23	0.0013669065840852584	0.0	0.0	0.013365308822166973	3.03757018685613E-4
24	0.0013669065840852584	0.0	0.0	0.017162271555737133	3.03757018685613E-4
25	0.0013669065840852584	0.0	0.0	0.018529178139822394	3.03757018685613E-4
26	0.0013669065840852584	0.0	0.0	0.022629897892078168	3.03757018685613E-4
27	0.0013669065840852584	0.0	0.0	0.041007197522557756	3.03757018685613E-4
28	0.0015187850934280651	0.0	0.0	0.13608314437115462	3.03757018685613E-4
29	0.0015187850934280651	0.0	0.0	0.27034374663019556	3.03757018685613E-4
30	0.0015187850934280651	0.0	0.0	0.4374101069072827	3.03757018685613E-4
31	0.0015187850934280651	0.0	0.0	1.0137890498632334	3.03757018685613E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGTG	25	0.0054946598	29.599998	37
GGTATCA	650	0.0	26.753845	1
TATCCTA	75	1.371518E-8	24.666666	18
TATTAGA	115	0.0	24.130436	2
GTACTAT	55	1.9010582E-5	23.545454	1
AGTTCCG	40	0.0019303162	23.125	32
CGGAATG	65	2.6799553E-6	22.76923	14
ATTAGAG	125	0.0	22.199999	3
ATATAGC	50	2.7004041E-4	22.199999	6
TCTATGG	255	0.0	21.039215	2
GCGGTAA	335	0.0	20.985075	23
GACAGGC	515	0.0	20.834951	7
CTTATAC	580	0.0	20.732758	37
CTAAGCC	45	0.0038240978	20.555557	4
ATAGCAA	55	5.139886E-4	20.181818	8
TAGCAAG	55	5.139886E-4	20.181818	9
GCTCTAG	55	5.139886E-4	20.181818	1
GTATTAG	230	0.0	20.108696	1
CATCAGA	510	0.0	19.950981	16
ACTATAT	65	6.896406E-5	19.923077	3
>>END_MODULE