FastQCFastQC Report
Fri 10 Feb 2017
ERR1631837.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631837.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1546506
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35610.23026098831818304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30970.20025787161511172No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29300.18945933607758392No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA26370.17051340246982552No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA26270.16986678357536278No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA25330.16378856596741298No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA17010.10998987394811272No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA16990.10986055016922015No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG16600.10733873648081547No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC15900.10281240421957626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18900.030.1481481
GCGGTAA11050.019.92307723
CTTATAC17400.019.88218337
ATACGGC1403.6379788E-1219.8214329
CGCGGTA11250.019.5688922
GCGTAAT951.6770537E-719.4736841
ATAATAC609.2387846E-418.53
CGTAATG801.6172595E-518.52
ATACGTC701.2195429E-418.529
TACACTT701.2195429E-418.55
TAGTACA701.2195429E-418.54
GTATCAA30900.018.2605172
CCGCGGT12700.017.91732221
TCTTACC1552.1827873E-1117.9032272
GGTAATA12550.017.83665325
AGCCGCG12500.017.7619
TATAGTG1156.409209E-817.6956525
CGGTAAT12750.017.41176424
CGTGCCA12950.017.28571510
GACCGTT1301.3940735E-817.0769237
Produced by FastQC (version 0.11.5)