##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631837.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1546506
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.672042009536334	34.0	31.0	34.0	31.0	34.0
2	32.83461751845773	34.0	31.0	34.0	31.0	34.0
3	32.92361103028375	34.0	31.0	34.0	31.0	34.0
4	36.32766507210447	37.0	37.0	37.0	35.0	37.0
5	36.24872842394404	37.0	37.0	37.0	35.0	37.0
6	36.29987597849604	37.0	37.0	37.0	35.0	37.0
7	36.284962360314154	37.0	37.0	37.0	35.0	37.0
8	36.2689236252559	37.0	37.0	37.0	35.0	37.0
9	38.098858329679935	39.0	38.0	39.0	37.0	39.0
10	38.04308486355695	39.0	38.0	39.0	35.0	39.0
11	38.11254789829461	39.0	38.0	39.0	37.0	39.0
12	38.05625002424821	39.0	38.0	39.0	37.0	39.0
13	38.10353015119243	39.0	38.0	39.0	37.0	39.0
14	39.51864266934626	41.0	39.0	41.0	37.0	41.0
15	39.52261549583383	41.0	39.0	41.0	37.0	41.0
16	39.48129913495324	41.0	39.0	41.0	37.0	41.0
17	39.45333804071888	41.0	39.0	41.0	37.0	41.0
18	39.47363928752944	41.0	39.0	41.0	37.0	41.0
19	39.4982955125942	41.0	39.0	41.0	37.0	41.0
20	39.48581900102554	41.0	39.0	41.0	37.0	41.0
21	39.45620450227804	41.0	39.0	41.0	37.0	41.0
22	39.43061067981631	41.0	39.0	41.0	37.0	41.0
23	39.36503511787216	41.0	39.0	41.0	36.0	41.0
24	39.379324102202	41.0	39.0	41.0	36.0	41.0
25	39.32436408264824	41.0	39.0	41.0	36.0	41.0
26	39.26651884958739	40.0	39.0	41.0	36.0	41.0
27	39.155495032027034	40.0	39.0	41.0	36.0	41.0
28	39.151231227036945	40.0	39.0	41.0	36.0	41.0
29	39.12408939894187	40.0	39.0	41.0	35.0	41.0
30	39.073485004261215	40.0	39.0	41.0	35.0	41.0
31	38.98767867696601	40.0	39.0	41.0	35.0	41.0
32	38.93060162715179	40.0	38.0	41.0	35.0	41.0
33	38.87325946359083	40.0	38.0	41.0	35.0	41.0
34	38.83272874466701	40.0	38.0	41.0	35.0	41.0
35	38.76178171956656	40.0	38.0	41.0	35.0	41.0
36	38.70614856974367	40.0	38.0	41.0	35.0	41.0
37	38.66028971112947	40.0	38.0	41.0	35.0	41.0
38	38.613781647145245	40.0	38.0	41.0	35.0	41.0
39	38.53525366212611	40.0	38.0	41.0	35.0	41.0
40	38.224012709940986	40.0	38.0	41.0	34.0	41.0
41	38.27759866434401	40.0	37.0	41.0	34.0	41.0
42	38.2547465059948	40.0	37.0	41.0	34.0	41.0
43	37.79819024303818	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	4.0
12	4.0
13	4.0
14	1.0
15	2.0
16	1.0
17	7.0
18	14.0
19	33.0
20	66.0
21	118.0
22	245.0
23	452.0
24	763.0
25	1292.0
26	2024.0
27	3078.0
28	4790.0
29	7067.0
30	9982.0
31	13811.0
32	18689.0
33	25098.0
34	36704.0
35	55657.0
36	85765.0
37	158002.0
38	346599.0
39	776228.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.10426212378096	16.99527838883263	12.765291566925702	27.135167920460702
2	19.91114163152293	20.022748052707197	34.042415613001175	26.02369470276869
3	20.624426934004784	20.660443606426355	29.68717871123681	29.02795074833205
4	15.041842708660685	16.16256257654351	34.42553730797036	34.37005740682545
5	16.32803235163653	33.83368703386861	33.45431572848731	16.383964886007554
6	33.439055522577995	34.57225513512395	15.851021593191362	16.137667749106697
7	29.77945122747665	28.904381877600215	20.380328301345095	20.93583859357804
8	26.767629740848083	32.12312141045686	20.372827522169327	20.73642132652573
9	27.590516945941367	13.828397691311899	18.293689128913822	40.287396233832915
10	18.724725283962687	24.40876401384799	30.664866479664482	26.201644222524838
11	37.70253720321809	20.66626317647652	20.0357450924859	21.595454527819484
12	22.295936776190974	24.276013154814787	27.908200808790912	25.519849260203326
13	33.64940064894672	18.338629142078982	22.437869623525547	25.574100585448743
14	23.64252062390964	20.311140079637582	24.31991857774881	31.726420718703967
15	28.144927986053723	25.017879012431894	21.22520054885012	25.61199245266426
16	24.854413755911715	24.866246881680382	23.28403510881949	26.995304253588408
17	25.546360634876297	24.484159776942345	23.346433832135148	26.62304575604621
18	25.745971887596948	22.60327473672912	24.6976086740045	26.95314470166944
19	27.24315327583598	23.270262126367438	24.58212253945345	24.904462058343128
20	28.07295930310002	22.234443319327568	24.208764789790663	25.483832587781745
21	26.516741609796533	23.241099614227167	23.424416070807354	26.81774270516894
22	26.683375298899588	23.86120713401694	22.977343767175814	26.478073799907666
23	26.66236018482954	23.258299676819878	23.917656963503536	26.161683174847045
24	26.697018957572748	22.76182568965138	23.989173013231117	26.551982339544754
25	26.934263429951127	23.231077021362996	24.20326852918773	25.631391019498146
26	26.690229459180888	23.532401426182634	23.857068773092376	25.9203003415441
27	26.272513653357954	23.095028405968034	24.681637187311267	25.95082075336274
28	25.630550414935342	24.10291327676711	23.680735800572386	26.585800507725153
29	26.014060081241198	24.414583583898157	23.57585421589053	25.995502118970116
30	25.731034991134855	23.98406472396486	24.725089977019167	25.55981030788112
31	26.576424533755443	23.47847341038444	23.914035897694543	26.031066158165565
32	24.274331945689188	23.633597283166054	24.647819019130868	27.444251752013894
33	24.865341615228136	23.126001451012797	25.744872635476355	26.26378429828271
34	25.06928521454168	23.03819060514476	25.336468141733686	26.556056038579868
35	25.037665550602455	24.01316257421568	25.50898606277635	25.440185812405513
36	25.093533423084036	23.236831929523714	25.92534396892091	25.744290678471344
37	25.6113458337698	22.53641434304167	25.43352563779255	26.41871418539598
38	24.544424657906276	22.280223937055528	26.516935595464876	26.65841580957332
39	24.19356924577079	21.55730401304618	27.4919075645358	26.75721917664723
40	23.989237675120563	22.222351546001114	28.1774529164452	25.610957862433125
41	22.814654453328988	21.639553936421844	28.973958070644407	26.57183353960476
42	21.989116110768403	21.97650704232638	29.38701822042721	26.647358626478006
43	21.196038036709847	22.382907017496215	29.263320025916485	27.157734919877452
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	33.0
1	34.5
2	36.0
3	101.5
4	167.0
5	167.0
6	260.0
7	353.0
8	355.0
9	357.0
10	583.5
11	810.0
12	810.0
13	1596.5
14	2383.0
15	4549.0
16	6715.0
17	6809.5
18	6904.0
19	6904.0
20	7685.5
21	8467.0
22	8056.5
23	7646.0
24	8715.0
25	9784.0
26	9784.0
27	10879.0
28	11974.0
29	15298.0
30	18622.0
31	21423.5
32	24225.0
33	24225.0
34	28273.0
35	32321.0
36	35567.0
37	38813.0
38	45064.0
39	51315.0
40	51315.0
41	58746.0
42	66177.0
43	72061.0
44	77945.0
45	95823.5
46	113702.0
47	113702.0
48	123930.0
49	134158.0
50	143681.0
51	153204.0
52	156344.0
53	159484.0
54	159484.0
55	146230.0
56	132976.0
57	124412.5
58	115849.0
59	106624.5
60	97400.0
61	97400.0
62	89504.0
63	81608.0
64	69182.5
65	56757.0
66	50137.5
67	43518.0
68	43518.0
69	37909.0
70	32300.0
71	27832.5
72	23365.0
73	19148.0
74	14931.0
75	14931.0
76	12125.5
77	9320.0
78	7568.5
79	5817.0
80	4554.5
81	3292.0
82	3292.0
83	2575.0
84	1858.0
85	1442.0
86	1026.0
87	810.5
88	595.0
89	595.0
90	420.0
91	245.0
92	142.5
93	40.0
94	26.5
95	13.0
96	13.0
97	7.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1546506.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.74863276584017
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.17722929672415	44.241251951228975
2	6.7922012807537335	6.893898569372809
3	1.982034731525884	3.017566581580431
4	0.9662236947887269	1.9613812582594536
5	0.544180523966432	1.380820878454747
6	0.3672364272010969	1.1182047949360598
7	0.27593308601866756	0.9802258795214445
8	0.2120639038268416	0.8609562542559065
9	0.16674018457154124	0.76156527547166
>10	1.1998369927650225	12.295918082923423
>50	0.1687728906964002	5.938955219084867
>100	0.13118134356670913	12.654250609742135
>500	0.011507093152620239	4.0169946626920625
>1k	0.004858550442217434	3.878009982476159
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3561	0.23026098831818304	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3097	0.20025787161511172	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2930	0.18945933607758392	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2637	0.17051340246982552	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2627	0.16986678357536278	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2533	0.16378856596741298	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1701	0.10998987394811272	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	1699	0.10986055016922015	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1660	0.10733873648081547	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	1590	0.10281240421957626	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.466188944627437E-5
2	0.0	0.0	0.0	0.0	6.466188944627437E-5
3	0.0	0.0	0.0	0.0	6.466188944627437E-5
4	0.0	0.0	0.0	6.466188944627437E-5	6.466188944627437E-5
5	0.0	0.0	0.0	6.466188944627437E-5	6.466188944627437E-5
6	0.0	0.0	0.0	6.466188944627437E-5	6.466188944627437E-5
7	0.0	0.0	0.0	6.466188944627437E-5	6.466188944627437E-5
8	6.466188944627437E-5	0.0	0.0	1.2932377889254875E-4	6.466188944627437E-5
9	6.466188944627437E-5	0.0	0.0	1.2932377889254875E-4	6.466188944627437E-5
10	1.2932377889254875E-4	0.0	0.0	1.2932377889254875E-4	6.466188944627437E-5
11	1.2932377889254875E-4	0.0	0.0	1.2932377889254875E-4	6.466188944627437E-5
12	1.9398566833882312E-4	0.0	0.0	2.586475577850975E-4	6.466188944627437E-5
13	1.9398566833882312E-4	0.0	0.0	3.2330944723137187E-4	6.466188944627437E-5
14	1.9398566833882312E-4	0.0	0.0	3.2330944723137187E-4	6.466188944627437E-5
15	1.9398566833882312E-4	0.0	0.0	3.8797133667764624E-4	6.466188944627437E-5
16	1.9398566833882312E-4	0.0	0.0	9.699283416941156E-4	6.466188944627437E-5
17	1.9398566833882312E-4	0.0	0.0	0.001551885346710585	1.2932377889254875E-4
18	4.526332261239206E-4	0.0	0.0	0.00206918046228078	1.2932377889254875E-4
19	4.526332261239206E-4	0.0	0.0	0.0028451231356360724	1.2932377889254875E-4
20	4.526332261239206E-4	0.0	0.0	0.0035564039195450908	1.2932377889254875E-4
21	4.526332261239206E-4	0.0	0.0	0.005690246271272145	1.2932377889254875E-4
22	4.526332261239206E-4	0.0	0.0	0.008535369406908218	1.2932377889254875E-4
23	4.526332261239206E-4	0.0	0.0	0.010992521205866643	1.2932377889254875E-4
24	4.526332261239206E-4	0.0	0.0	0.014160953788734089	1.2932377889254875E-4
25	4.526332261239206E-4	0.0	0.0	0.015583515356552124	1.2932377889254875E-4
26	4.526332261239206E-4	0.0	0.0	0.017911343376618004	1.2932377889254875E-4
27	4.526332261239206E-4	0.0	0.0	0.03776254343662423	1.2932377889254875E-4
28	4.526332261239206E-4	0.0	0.0	0.13288018281209385	1.2932377889254875E-4
29	4.526332261239206E-4	0.0	0.0	0.2838656946691445	1.2932377889254875E-4
30	4.526332261239206E-4	0.0	0.0	0.48037317669637236	1.2932377889254875E-4
31	4.526332261239206E-4	0.0	0.0	1.0622655198233955	1.2932377889254875E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1890	0.0	30.148148	1
GCGGTAA	1105	0.0	19.923077	23
CTTATAC	1740	0.0	19.882183	37
ATACGGC	140	3.6379788E-12	19.82143	29
CGCGGTA	1125	0.0	19.56889	22
GCGTAAT	95	1.6770537E-7	19.473684	1
ATAATAC	60	9.2387846E-4	18.5	3
CGTAATG	80	1.6172595E-5	18.5	2
ATACGTC	70	1.2195429E-4	18.5	29
TACACTT	70	1.2195429E-4	18.5	5
TAGTACA	70	1.2195429E-4	18.5	4
GTATCAA	3090	0.0	18.260517	2
CCGCGGT	1270	0.0	17.917322	21
TCTTACC	155	2.1827873E-11	17.903227	2
GGTAATA	1255	0.0	17.836653	25
AGCCGCG	1250	0.0	17.76	19
TATAGTG	115	6.409209E-8	17.695652	5
CGGTAAT	1275	0.0	17.411764	24
CGTGCCA	1295	0.0	17.285715	10
GACCGTT	130	1.3940735E-8	17.076923	7
>>END_MODULE