FastQCFastQC Report
Fri 10 Feb 2017
ERR1631836.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631836.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211513
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4790.22646362162136607No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4700.22220856401261388No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4500.21275288043760907No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3940.18627696642759545No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3380.1598010524175819No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3340.15790991570258092No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA3310.1564915631663302No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC3290.15554599480882972No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3210.15176372137882777No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2920.13805298019507076No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2670.12623337572631468No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2510.11866882886631082No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2290.10826757693380548No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2220.1049580876825538No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2220.1049580876825538No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT2200.1040125193250533No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2130.10070303007380162No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2120.10023024589505138No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATTA200.001838542537.015
CAGTATT200.001838542537.014
GGTATCA2700.031.5185181
TAAAAGT250.005486334729.5999984
CCGTCCA250.005486334729.5999989
ATTCCTT250.005486334729.5999986
AAAGCGG250.005486334729.5999985
TAAACTA358.8453875E-426.428575
CTAAACT358.8453875E-426.428574
CCTAAAC400.001926009323.1253
GTATCAA3700.023.02
TATTAGA502.692014E-422.1999992
TTATACA1151.3278623E-1020.91304437
GCGGTAA1351.8189894E-1220.55555523
TCCTAAA450.003815648420.5555552
CTACCAA450.003815648420.5555559
TATACAC450.003815648420.55555537
CCTGCAT450.003815648420.55555517
GGTTAAA450.003815648420.5555551
TTCTGCG909.386895E-820.55555518
Produced by FastQC (version 0.11.5)