FastQCFastQC Report
Fri 10 Feb 2017
ERR1631835.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631835.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388190
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA11650.30011077049898244No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA10680.2751230067750328No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA9240.23802776990648908No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC8930.2300419897472887No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8580.22102578634173986No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT8530.21973775728380435No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG8500.218964939849043No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7430.19140111800922227No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7290.18779463664700277No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7240.1865066075890672No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6870.17697519256034416No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA6840.17620237512558284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5680.14632010098147816No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5580.14374404286560705No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5520.1421984079960844No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5450.14039516731497462No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA5230.13472783946005823No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5220.1344702336484711No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT4900.1262268476776836No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA4870.1254540302429223No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4700.12107473144594141No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4700.12107473144594141No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4440.11437698034467657No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4400.11334655709832814No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4390.11308895128674104No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4320.11128571060563126No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4230.10896725830134729No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT4220.10870965248976018No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4200.10819444086658594No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA4140.1066488059970633No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4140.1066488059970633No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA4100.10561838275071486No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG4060.10458795950436643No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3950.10175429557690821No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3940.1014966897653211No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGTT303.5951263E-430.83333214
GGTATCA5500.028.2545431
GTATATC358.858773E-426.428571
CCGTTAA358.858773E-426.4285716
CCGCTTA451.3210071E-424.66666825
TATACAC1150.024.13043237
GTTTTAT551.8984525E-523.5454541
CTAAACC400.001928896323.1254
CCATAGC502.6976372E-422.1999991
GAACAGT603.7181082E-521.5833326
GTATTAG705.0875715E-621.1428571
CTTATAC3500.020.61428637
GGTACCG450.003821311820.5555576
ACCGGTA450.003821311820.55555728
GGGCTAA450.003821311820.5555571
CGTTAAA450.003821311820.55555717
GTTAAAC450.003821311820.55555718
TAACATA450.003821311820.55555726
ACCGTTT450.003821311820.55555710
TACCGCA450.003821311820.5555577
Produced by FastQC (version 0.11.5)