##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631835.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	388190
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.653159535279116	34.0	31.0	34.0	31.0	34.0
2	32.816793322857365	34.0	31.0	34.0	31.0	34.0
3	32.90224632267704	34.0	31.0	34.0	31.0	34.0
4	36.30776166310312	37.0	37.0	37.0	35.0	37.0
5	36.22572966846132	37.0	35.0	37.0	35.0	37.0
6	36.28142662098457	37.0	37.0	37.0	35.0	37.0
7	36.270717947396896	37.0	37.0	37.0	35.0	37.0
8	36.250233133259485	37.0	37.0	37.0	35.0	37.0
9	38.087050155851514	39.0	38.0	39.0	37.0	39.0
10	38.024016589814266	39.0	38.0	39.0	35.0	39.0
11	38.098456941188594	39.0	38.0	39.0	37.0	39.0
12	38.03739405960999	39.0	38.0	39.0	35.0	39.0
13	38.08778175635642	39.0	38.0	39.0	37.0	39.0
14	39.48694711352688	41.0	39.0	41.0	37.0	41.0
15	39.49592725211881	41.0	39.0	41.0	37.0	41.0
16	39.434248692650506	41.0	39.0	41.0	37.0	41.0
17	39.412215667585464	41.0	39.0	41.0	37.0	41.0
18	39.43813081223112	41.0	39.0	41.0	37.0	41.0
19	39.452796311084775	41.0	39.0	41.0	37.0	41.0
20	39.45371338777403	41.0	39.0	41.0	37.0	41.0
21	39.415227079522914	41.0	39.0	41.0	37.0	41.0
22	39.39013884953245	40.0	39.0	41.0	37.0	41.0
23	39.32934130193977	40.0	39.0	41.0	36.0	41.0
24	39.34278059713027	41.0	39.0	41.0	36.0	41.0
25	39.28752930266107	40.0	39.0	41.0	36.0	41.0
26	39.21948530358845	40.0	39.0	41.0	36.0	41.0
27	39.11513691748886	40.0	39.0	41.0	36.0	41.0
28	39.11995929828177	40.0	39.0	41.0	35.0	41.0
29	39.082129884850204	40.0	39.0	41.0	35.0	41.0
30	39.04974625827559	40.0	39.0	41.0	35.0	41.0
31	38.95313377469796	40.0	38.0	41.0	35.0	41.0
32	38.91229810144517	40.0	38.0	41.0	35.0	41.0
33	38.8479636260594	40.0	38.0	41.0	35.0	41.0
34	38.82194286303099	40.0	38.0	41.0	35.0	41.0
35	38.753641258146786	40.0	38.0	41.0	35.0	41.0
36	38.69937144181973	40.0	38.0	41.0	35.0	41.0
37	38.65580514696411	40.0	38.0	41.0	35.0	41.0
38	38.613050310415005	40.0	38.0	41.0	35.0	41.0
39	38.535997836111186	40.0	38.0	41.0	35.0	41.0
40	38.231878719183904	40.0	38.0	41.0	34.0	41.0
41	38.30403153095134	40.0	38.0	41.0	34.0	41.0
42	38.288722017568716	40.0	37.0	41.0	34.0	41.0
43	37.81174167289214	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	3.0
19	8.0
20	7.0
21	24.0
22	50.0
23	105.0
24	191.0
25	334.0
26	502.0
27	789.0
28	1159.0
29	1768.0
30	2511.0
31	3474.0
32	4770.0
33	6454.0
34	9337.0
35	14409.0
36	21904.0
37	39358.0
38	90462.0
39	190568.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.251732399082925	16.539065921327182	13.302506504546743	27.90669517504315
2	20.501300909348515	19.900048945104203	32.83134547515392	26.767304670393365
3	20.78054560910894	20.072387233055977	29.758880960354468	29.388186197480614
4	15.409979649140885	16.564311290862722	34.222932069347486	33.80277699064891
5	16.74798423452433	32.66647775573817	33.69535536721708	16.890182642520415
6	33.0036837631057	33.981813029701954	16.080269970890544	16.93423323630181
7	29.860635255931374	27.82529173858162	20.580385893505756	21.733687111981244
8	27.022849635487777	30.575491383085602	20.854993688657615	21.546665292769003
9	28.60995904067596	12.9596331693243	18.08908008964682	40.34132770035292
10	19.36087998145238	23.938792859166902	29.74960715113733	26.950720008243383
11	38.43633272366625	19.91524768798784	18.912388263479222	22.73603132486669
12	22.5899688296968	24.208763749710194	26.939127746721965	26.262139673871044
13	34.859733635590814	17.957701125737398	21.325897112238852	25.85666812643293
14	23.982328241325124	20.885133568613305	23.66624591050774	31.466292279553826
15	29.488394858188	24.528710167701384	20.400834642829544	25.582060331281077
16	24.775238929390248	24.56657822200469	22.984620933048248	27.673561915556817
17	26.51382055179165	24.052912233699992	22.13787063036142	27.295396584146935
18	26.66168628764265	21.531724155696953	23.60983023777016	28.196759318890237
19	27.906179963419973	22.662098456941187	23.825446302068578	25.606275277570262
20	29.87686442206136	20.97246193874134	23.28061001056184	25.870063628635464
21	27.068961075761873	22.58352868440712	22.414539272005975	27.93297096782503
22	27.84821865581287	23.072464514799453	22.06677142636338	27.01254540302429
23	27.6099332800948	22.489502563177822	22.87513846312373	27.02542569360365
24	27.37190551018831	21.90473737087509	23.72781369947706	26.995543419459544
25	27.514103918184396	22.293206934748447	23.65542646642108	26.537262680646073
26	27.488085731214095	23.134805121203534	22.843968159921687	26.53314098766068
27	26.443236559416782	22.04822380792911	24.776269352636596	26.732270280017516
28	25.74821607975476	23.24840928411345	22.68064607537546	28.32272856075633
29	26.768850305262887	23.77959246760607	22.720574976171463	26.730982250959585
30	26.47337643937247	22.49980679564131	23.953991602050543	27.072825162935676
31	27.634148226383985	22.767459233880317	23.143306112985908	26.455086426749787
32	25.485715757747496	22.59074164713156	23.304052139416264	28.619490455704682
33	25.528478322470953	22.04770859630593	25.230686004276254	27.193127076946855
34	26.079497153455783	22.221334913315644	24.192792189391792	27.50637574383678
35	25.42543599783611	24.12993637136454	24.81516783018625	25.629459800613102
36	26.006337102965045	23.032535614003454	24.833200236997346	26.12792704603416
37	26.730982250959585	22.745562739895412	24.38908781782117	26.134367191323836
38	25.763672428449986	22.22957829928643	25.12171874597491	26.885030526288674
39	25.444756433705145	21.200185476184345	26.074860248847216	27.2801978412633
40	25.1418119992787	22.243746618923723	26.578994822123185	26.035446559674387
41	23.559597104510676	21.348566423658518	27.596537777892273	27.495298693938537
42	22.23447280970659	21.641979443056236	28.42886215513022	27.69468559210696
43	21.64249465467941	22.553131198639843	28.218655812875138	27.58571833380561
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6.0
1	5.5
2	5.0
3	10.5
4	16.0
5	16.0
6	26.5
7	37.0
8	39.0
9	41.0
10	72.0
11	103.0
12	103.0
13	212.5
14	322.0
15	640.0
16	958.0
17	918.5
18	879.0
19	879.0
20	1060.5
21	1242.0
22	1048.5
23	855.0
24	944.0
25	1033.0
26	1033.0
27	1256.5
28	1480.0
29	2011.0
30	2542.0
31	2919.0
32	3296.0
33	3296.0
34	4079.5
35	4863.0
36	5455.0
37	6047.0
38	7492.5
39	8938.0
40	8938.0
41	10921.0
42	12904.0
43	14938.5
44	16973.0
45	24059.5
46	31146.0
47	31146.0
48	34186.0
49	37226.0
50	42104.5
51	46983.0
52	47320.5
53	47658.0
54	47658.0
55	42259.0
56	36860.0
57	34637.0
58	32414.0
59	29460.0
60	26506.0
61	26506.0
62	24144.0
63	21782.0
64	18008.0
65	14234.0
66	12285.5
67	10337.0
68	10337.0
69	8834.0
70	7331.0
71	6297.0
72	5263.0
73	4104.0
74	2945.0
75	2945.0
76	2407.5
77	1870.0
78	1564.5
79	1259.0
80	1028.0
81	797.0
82	797.0
83	659.0
84	521.0
85	388.5
86	256.0
87	207.5
88	159.0
89	159.0
90	115.0
91	71.0
92	48.5
93	26.0
94	15.0
95	4.0
96	4.0
97	2.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	388190.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.172749578286684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.88583824961118	44.46187242646875
2	6.198383794933631	6.343766834564979
3	2.0601703972267202	3.162737514776471
4	1.0463566429508249	2.141797858371972
5	0.677910094623374	1.7345261754377272
6	0.41206556669829114	1.2651916832691865
7	0.3214696164160043	1.1515338924518848
8	0.2612661975566098	1.0695767760668455
9	0.22884540105534007	1.053958356031275
>10	1.6073371571693231	16.279127988923864
>50	0.17423738166103664	6.1705847045205555
>100	0.11700242780145167	11.680229727580706
>500	0.008104064263304013	2.906320254202078
>1k	0.0010130080329130017	0.5787758073337413
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1165	0.30011077049898244	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1068	0.2751230067750328	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	924	0.23802776990648908	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	893	0.2300419897472887	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	858	0.22102578634173986	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	853	0.21973775728380435	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	850	0.218964939849043	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	743	0.19140111800922227	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	729	0.18779463664700277	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	724	0.1865066075890672	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	687	0.17697519256034416	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	684	0.17620237512558284	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	568	0.14632010098147816	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	558	0.14374404286560705	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	552	0.1421984079960844	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	545	0.14039516731497462	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	523	0.13472783946005823	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	522	0.1344702336484711	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	490	0.1262268476776836	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	487	0.1254540302429223	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	470	0.12107473144594141	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	470	0.12107473144594141	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	444	0.11437698034467657	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	440	0.11334655709832814	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	439	0.11308895128674104	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	432	0.11128571060563126	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	423	0.10896725830134729	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	422	0.10870965248976018	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	420	0.10819444086658594	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	414	0.1066488059970633	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	414	0.1066488059970633	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	410	0.10561838275071486	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	406	0.10458795950436643	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	395	0.10175429557690821	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	394	0.1014966897653211	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.576058115871094E-4	0.0	0.0	0.0	0.0
6	2.576058115871094E-4	0.0	0.0	0.0	0.0
7	2.576058115871094E-4	0.0	0.0	0.0	0.0
8	5.152116231742188E-4	0.0	0.0	0.0	0.0
9	5.152116231742188E-4	0.0	0.0	0.0	0.0
10	5.152116231742188E-4	0.0	0.0	0.0	0.0
11	5.152116231742188E-4	0.0	0.0	0.0	0.0
12	5.152116231742188E-4	0.0	0.0	0.0	0.0
13	5.152116231742188E-4	0.0	0.0	0.0	0.0
14	5.152116231742188E-4	0.0	0.0	0.0	0.0
15	5.152116231742188E-4	0.0	0.0	5.152116231742188E-4	0.0
16	5.152116231742188E-4	0.0	0.0	0.001288029057935547	0.0
17	7.728174347613283E-4	0.0	0.0	0.0015456348695226565	0.0
18	0.0010304232463484377	0.0	0.0	0.0020608464926968753	0.0
19	0.0010304232463484377	0.0	0.0	0.0028336639274582033	0.0
20	0.0010304232463484377	0.0	0.0	0.003864087173806641	0.0
21	0.0010304232463484377	0.0	0.0	0.005409722043329297	0.0
22	0.0010304232463484377	0.0	0.0	0.007212962724439063	0.0
23	0.0010304232463484377	0.0	0.0	0.00850099178237461	0.0
24	0.0010304232463484377	0.0	0.0	0.012365078956181252	0.0
25	0.0010304232463484377	0.0	0.0	0.01313789639094258	0.0
26	0.0010304232463484377	0.0	0.0	0.01700198356474922	0.0
27	0.0010304232463484377	0.0	0.0	0.03503439037584688	0.0
28	0.0010304232463484377	0.0	0.0	0.09995105489579845	0.0
29	0.0010304232463484377	0.0	0.0	0.21948015147221722	0.0
30	0.0010304232463484377	0.0	0.0	0.3758468791055926	0.0
31	0.001288029057935547	0.0	0.0	0.7638012313557794	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCGTT	30	3.5951263E-4	30.833332	14
GGTATCA	550	0.0	28.254543	1
GTATATC	35	8.858773E-4	26.42857	1
CCGTTAA	35	8.858773E-4	26.42857	16
CCGCTTA	45	1.3210071E-4	24.666668	25
TATACAC	115	0.0	24.130432	37
GTTTTAT	55	1.8984525E-5	23.545454	1
CTAAACC	40	0.0019288963	23.125	4
CCATAGC	50	2.6976372E-4	22.199999	1
GAACAGT	60	3.7181082E-5	21.583332	6
GTATTAG	70	5.0875715E-6	21.142857	1
CTTATAC	350	0.0	20.614286	37
GGTACCG	45	0.0038213118	20.555557	6
ACCGGTA	45	0.0038213118	20.555557	28
GGGCTAA	45	0.0038213118	20.555557	1
CGTTAAA	45	0.0038213118	20.555557	17
GTTAAAC	45	0.0038213118	20.555557	18
TAACATA	45	0.0038213118	20.555557	26
ACCGTTT	45	0.0038213118	20.555557	10
TACCGCA	45	0.0038213118	20.555557	7
>>END_MODULE