FastQCFastQC Report
Fri 10 Feb 2017
ERR1631834.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631834.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900858
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA19040.2113540646805601No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18830.20902295367305393No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA18160.20158559950624852No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA17220.19115110261550655No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17140.19026306032693277No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA16110.17882951586154533No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG14330.1590705749407787No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA14320.15895956965470698No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT14090.15640644807505732No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC13970.15507438464219667No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13530.15019015205504088No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC12670.1406436974528727No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12520.13897861816179685No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA12250.13598147543786035No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA11300.1254359732610467No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT11180.12410390982818603No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT11110.12332687282568397No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC10010.11111629135779445No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9110.1011258156113394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13600.029.7904421
ATACGGC803.274181E-1127.7529
TATTAGA753.7389145E-722.22
ATTAGAG705.0999006E-621.1428593
CTAATCA450.00382517620.5555554
CGGTACA555.141906E-420.181824
GTATCAA20500.019.7634142
CCGGTAC759.262127E-619.7333343
AGTCGGT3500.019.55714211
CAGTCGG3600.019.52777710
CTTATAC8150.019.52147137
CTCTATG5200.019.2115381
TATACAC2200.018.50000237
GCTATGA609.2347036E-418.51
GCAGTCG3800.018.59
TACGGCT1205.169568E-918.530
CTACGAA500.007033729518.527
TCTATGG5450.017.9908262
CATTGTA953.604635E-617.5263162
CTTATTG3800.017.52631628
Produced by FastQC (version 0.11.5)