##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631834.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	900858
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.66634697144278	34.0	31.0	34.0	31.0	34.0
2	32.83011640014298	34.0	31.0	34.0	31.0	34.0
3	32.918911748577464	34.0	31.0	34.0	31.0	34.0
4	36.32324961314658	37.0	37.0	37.0	35.0	37.0
5	36.24750404614268	37.0	37.0	37.0	35.0	37.0
6	36.29950891261442	37.0	37.0	37.0	35.0	37.0
7	36.288561571302026	37.0	37.0	37.0	35.0	37.0
8	36.27336827779739	37.0	37.0	37.0	35.0	37.0
9	38.10600227782847	39.0	38.0	39.0	37.0	39.0
10	38.04892335973039	39.0	38.0	39.0	35.0	39.0
11	38.114688441463585	39.0	38.0	39.0	37.0	39.0
12	38.05687910858315	39.0	38.0	39.0	37.0	39.0
13	38.102726511836494	39.0	38.0	39.0	37.0	39.0
14	39.517604328318114	41.0	39.0	41.0	37.0	41.0
15	39.53001693940666	41.0	39.0	41.0	37.0	41.0
16	39.47641359681548	41.0	39.0	41.0	37.0	41.0
17	39.45321349202649	41.0	39.0	41.0	37.0	41.0
18	39.474469894256366	41.0	39.0	41.0	37.0	41.0
19	39.4980218858022	41.0	39.0	41.0	37.0	41.0
20	39.4936871293811	41.0	39.0	41.0	37.0	41.0
21	39.45895468542212	41.0	39.0	41.0	37.0	41.0
22	39.44325853797158	41.0	39.0	41.0	37.0	41.0
23	39.37044351051997	41.0	39.0	41.0	36.0	41.0
24	39.38943984512542	41.0	39.0	41.0	36.0	41.0
25	39.34408530534224	41.0	39.0	41.0	36.0	41.0
26	39.29288633724738	40.0	39.0	41.0	36.0	41.0
27	39.18398349129386	40.0	39.0	41.0	36.0	41.0
28	39.174277189079746	40.0	39.0	41.0	36.0	41.0
29	39.148808136243446	40.0	39.0	41.0	35.0	41.0
30	39.1102626607079	40.0	39.0	41.0	35.0	41.0
31	39.020897855155866	40.0	39.0	41.0	35.0	41.0
32	38.97136729651066	40.0	38.0	41.0	35.0	41.0
33	38.910133450554916	40.0	38.0	41.0	35.0	41.0
34	38.878286033980935	40.0	38.0	41.0	35.0	41.0
35	38.80784762970413	40.0	38.0	41.0	35.0	41.0
36	38.76033736726543	40.0	38.0	41.0	35.0	41.0
37	38.71513268461844	40.0	38.0	41.0	35.0	41.0
38	38.66789438513062	40.0	38.0	41.0	35.0	41.0
39	38.58750879716892	40.0	38.0	41.0	35.0	41.0
40	38.27640871258289	40.0	38.0	41.0	34.0	41.0
41	38.33541912265862	40.0	38.0	41.0	34.0	41.0
42	38.322302738056386	40.0	38.0	41.0	34.0	41.0
43	37.868654105308494	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	0.0
15	0.0
16	2.0
17	4.0
18	7.0
19	8.0
20	38.0
21	69.0
22	124.0
23	238.0
24	412.0
25	703.0
26	1051.0
27	1745.0
28	2717.0
29	4031.0
30	5763.0
31	7887.0
32	10729.0
33	14468.0
34	20978.0
35	31859.0
36	49277.0
37	89970.0
38	203548.0
39	455228.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.7835463524773	16.781113116606612	13.041900055280633	27.39344047563545
2	20.130031592104416	20.066092547327102	33.40848391200389	26.39539194856459
3	21.21022403086835	20.456720149013496	29.591345139855562	28.741710680262596
4	15.339376461107076	16.52890910665166	34.07984388216567	34.0518705500756
5	16.61593725093189	33.258626775807066	33.463653539181536	16.661782434079512
6	33.122090273938845	33.85283807214899	16.078782671630822	16.946288982281335
7	29.76284830683637	28.32721694207078	20.391560046089392	21.518374705003453
8	26.609743155969092	31.51195859946851	20.40532470156229	21.47297354300012
9	27.99531113671633	13.624122780726818	18.339405322481454	40.04116076007539
10	19.092687193764167	24.301721247965826	30.23839495236763	26.36719660590237
11	37.625685735154704	20.506006496029343	19.72896949352728	22.13933827528867
12	22.228031498859977	24.50530494262137	27.295200797461977	25.971462761056678
13	33.80954601058102	18.346065639645758	21.987039022798267	25.857349326974948
14	23.657113551747333	20.79339918166903	23.93584782507343	31.613639441510205
15	28.710296184304298	24.9758563502794	20.902628383163606	25.411219082252696
16	24.83543466339867	24.85319550917015	23.371607955970862	26.93976187146032
17	25.875554193890714	24.614090122971653	22.751865443832436	26.758490239305193
18	25.696391662170953	22.503879634748206	24.469894256364487	27.32983444671635
19	27.265340375508686	23.30822393762391	24.584895732734793	24.841539954132617
20	28.493169844748007	21.914330560421288	24.11423331979069	25.478266275040017
21	26.366863590044158	23.349739914614734	23.283469758829916	26.999926736511192
22	26.957300706659655	23.685641910267766	22.75020036454136	26.606857018531223
23	26.568338184264334	23.209318227734006	23.87335184901505	26.34899173898661
24	27.007919117108358	22.786610098372883	23.961157030297784	26.244313754220975
25	26.897468857466993	23.070228604286136	24.08348485554882	25.94881768269805
26	26.777805159081673	23.605496093723982	23.56731027531531	26.04938847187903
27	26.169829207266847	22.761966924864964	24.849865350587997	26.218338517280195
28	25.521447331321916	23.72771291368895	23.50270519882157	27.248134556167564
29	26.09367958102165	24.286069502629715	23.507700436694794	26.11255047965384
30	25.867894829151766	23.351515999191882	24.70988768485155	26.070701486804804
31	26.843409283150066	23.315550286504642	23.949723485832394	25.891316944512898
32	24.83598968982903	23.392920970896633	24.21924432041454	27.5518450188598
33	25.145028406252706	23.044253367345352	25.31331241993744	26.497405806464503
34	25.530882780638013	23.09875696280657	24.763947259168482	26.606412997386936
35	25.14580544325521	24.143760725885766	25.075317086599664	25.635116744259363
36	25.458729344691395	23.10353019010765	25.419211462849862	26.018529002351094
37	26.083134078844832	22.737101740784897	25.007603862095912	26.172160318274358
38	25.255700676466216	22.33959181136206	26.008205510746425	26.396502001425308
39	24.814454664331116	21.466313225835815	26.90757033849952	26.81166177133355
40	24.695345992376158	22.24135213318858	27.3257272511317	25.737574623303562
41	23.006400564794895	21.933645480197768	28.1791358904511	26.880818064556234
42	22.168310654953387	22.17552599854805	28.552446667510306	27.103716678988256
43	21.455545713086856	22.88218564968064	28.364403712904807	27.297864924327698
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15.0
1	16.5
2	18.0
3	51.0
4	84.0
5	84.0
6	108.0
7	132.0
8	161.0
9	190.0
10	283.0
11	376.0
12	376.0
13	768.5
14	1161.0
15	2283.0
16	3405.0
17	3326.0
18	3247.0
19	3247.0
20	3817.0
21	4387.0
22	3809.0
23	3231.0
24	3420.5
25	3610.0
26	3610.0
27	4103.0
28	4596.0
29	6283.0
30	7970.0
31	9176.5
32	10383.0
33	10383.0
34	12633.5
35	14884.0
36	16257.0
37	17630.0
38	22739.5
39	27849.0
40	27849.0
41	32857.5
42	37866.0
43	41527.0
44	45188.0
45	57953.0
46	70718.0
47	70718.0
48	77349.5
49	83981.0
50	93874.0
51	103767.0
52	104165.0
53	104563.0
54	104563.0
55	91908.5
56	79254.0
57	72976.0
58	66698.0
59	61448.5
60	56199.0
61	56199.0
62	51644.5
63	47090.0
64	38892.0
65	30694.0
66	26709.0
67	22724.0
68	22724.0
69	19691.0
70	16658.0
71	14234.5
72	11811.0
73	9733.5
74	7656.0
75	7656.0
76	6341.5
77	5027.0
78	4072.5
79	3118.0
80	2582.0
81	2046.0
82	2046.0
83	1650.0
84	1254.0
85	999.5
86	745.0
87	561.5
88	378.0
89	378.0
90	279.5
91	181.0
92	118.0
93	55.0
94	35.5
95	16.0
96	16.0
97	9.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	900858.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.09693625134698
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.74462486047854	39.5256450608914
2	6.904394126078957	6.365428317680726
3	2.2572265618809104	3.1215368678361437
4	1.135363045700742	2.09347031759209
5	0.6931614869257455	1.5976310437352488
6	0.49467472573105303	1.3681793578306143
7	0.32531994682428417	1.0497376995035432
8	0.26072330698435275	0.9614836529038464
9	0.20052787441718184	0.8319348579264252
>10	1.5765394093500382	14.764188532286271
>50	0.22466377110901017	7.211804996353384
>100	0.1658343124449245	14.349365035423753
>500	0.012588882112325495	3.8828245049520573
>1k	0.004357689961958825	2.876769755084475
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1904	0.2113540646805601	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1883	0.20902295367305393	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1816	0.20158559950624852	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1722	0.19115110261550655	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1714	0.19026306032693277	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1611	0.17882951586154533	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1433	0.1590705749407787	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1432	0.15895956965470698	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1409	0.15640644807505732	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1397	0.15507438464219667	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1353	0.15019015205504088	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1267	0.1406436974528727	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1252	0.13897861816179685	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1225	0.13598147543786035	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1130	0.1254359732610467	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1118	0.12410390982818603	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1111	0.12332687282568397	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1001	0.11111629135779445	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	911	0.1011258156113394	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	1.1100528607172273E-4	0.0	0.0	0.0	0.0
9	1.1100528607172273E-4	0.0	0.0	0.0	0.0
10	2.2201057214344547E-4	0.0	0.0	0.0	0.0
11	2.2201057214344547E-4	0.0	0.0	0.0	0.0
12	2.2201057214344547E-4	0.0	0.0	0.0	0.0
13	2.2201057214344547E-4	0.0	0.0	0.0	0.0
14	2.2201057214344547E-4	0.0	0.0	0.0	0.0
15	2.2201057214344547E-4	0.0	0.0	4.4402114428689093E-4	0.0
16	2.2201057214344547E-4	0.0	0.0	5.550264303586137E-4	0.0
17	3.330158582151682E-4	0.0	0.0	8.880422885737819E-4	0.0
18	3.330158582151682E-4	0.0	0.0	0.0013320634328606727	0.0
19	3.330158582151682E-4	0.0	0.0	0.0018870898632192864	0.0
20	3.330158582151682E-4	0.0	0.0	0.0027751321517930686	0.0
21	3.330158582151682E-4	0.0	0.0	0.004440211442868909	0.0
22	3.330158582151682E-4	0.0	0.0	0.007104338308590255	0.0
23	3.330158582151682E-4	0.0	0.0	0.009102433457881264	0.0
24	3.330158582151682E-4	0.0	0.0	0.011877565609674333	0.0
25	3.330158582151682E-4	0.0	0.0	0.013209629042535006	0.0
26	3.330158582151682E-4	0.0	0.0	0.017205819341117024	0.0
27	3.330158582151682E-4	0.0	0.0	0.03896285541117468	0.0
28	3.330158582151682E-4	0.0	0.0	0.11888666138281505	0.0
29	3.330158582151682E-4	0.0	0.0	0.2432125817831445	0.0
30	3.330158582151682E-4	0.0	0.0	0.39950802457213014	0.0
31	3.330158582151682E-4	0.0	0.0	0.8500784807372527	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1360	0.0	29.790442	1
ATACGGC	80	3.274181E-11	27.75	29
TATTAGA	75	3.7389145E-7	22.2	2
ATTAGAG	70	5.0999006E-6	21.142859	3
CTAATCA	45	0.003825176	20.555555	4
CGGTACA	55	5.141906E-4	20.18182	4
GTATCAA	2050	0.0	19.763414	2
CCGGTAC	75	9.262127E-6	19.733334	3
AGTCGGT	350	0.0	19.557142	11
CAGTCGG	360	0.0	19.527777	10
CTTATAC	815	0.0	19.521471	37
CTCTATG	520	0.0	19.211538	1
TATACAC	220	0.0	18.500002	37
GCTATGA	60	9.2347036E-4	18.5	1
GCAGTCG	380	0.0	18.5	9
TACGGCT	120	5.169568E-9	18.5	30
CTACGAA	50	0.0070337295	18.5	27
TCTATGG	545	0.0	17.990826	2
CATTGTA	95	3.604635E-6	17.526316	2
CTTATTG	380	0.0	17.526316	28
>>END_MODULE