FastQCFastQC Report
Fri 10 Feb 2017
ERR1631833.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631833.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159259
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9270.5820707149988383No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA8250.5180240991089985No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7260.4558612072159188No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6690.42007045127747883No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC6110.38365178734011895No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6090.38239597134227893No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG6020.378000615349839No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5370.33718659542003904No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5360.33655868742111905No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC5270.33090751543083907No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4860.30516328747511917No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4750.29825629948699917No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4430.27816324352155924No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3530.2216515236187594No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3480.21851198362415938No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA3420.21474453563063942No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3390.21286081163387943No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3380.2122329036349594No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3270.2053259156468394No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3260.20469800764791943No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT3250.20407009964899941No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT3150.19779101965979948No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3150.19779101965979948No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3130.19653520366195945No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT3130.19653520366195945No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG3110.19527938766411945No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3030.19025612367275946No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC2910.1827212276857195No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG2910.1827212276857195No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC2810.1764421476965195No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC2810.1764421476965195No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT2800.17581423969759952No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2790.1751863316986795No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC2740.17204679170407952No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA2720.17079097570623952No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG2700.16953515970839952No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA2690.1689072517094795No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2680.16827934371055953No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA2660.16702352771271953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2660.16702352771271953No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2660.16702352771271953No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2640.16576771171487953No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2580.16200026372135953No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2530.15886072372675955No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA2520.15823281572783957No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2510.15760490772891955No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2440.15320955173647957No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2380.14944210374295958No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2370.14881419574403956No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC2330.1463025637483596No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC2300.14441883975159958No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2280.1431630237537596No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC2280.1431630237537596No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA2270.1425351157548396No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC2260.1419072077559196No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC2200.13813975976239962No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2150.13500021976779963No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC2130.1337444037699596No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC2090.13123277177427964No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC2050.12872113977859964No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC2050.12872113977859964No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT2020.12683741578183966No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG2020.12683741578183966No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC2020.12683741578183966No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT2000.12558159978399966No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT1950.12244205978939966No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA1950.12244205978939966No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT1940.12181415179047966No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC1930.12118624379155965No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1930.12118624379155965No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG1920.12055833579263968No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG1910.11993042779371967No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA1910.11993042779371967No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1890.11867461179587967No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG1870.11741879579803968No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC1810.11365134780451967No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG1800.11302343980559967No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT1790.11239553180667969No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1780.11176762380775969No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG1770.11113971580883969No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT1760.11051180780991968No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA1760.11051180780991968No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT1720.1080001758142397No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA1670.10486063581963971No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG1640.1029769118228797No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG1620.10172109582503971No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAA1610.10109318782611972No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAAGC251.2288561E-436.9999963
AAAGCGG251.2288561E-436.9999965
GATAAAG303.585692E-430.8333342
AGCGGGT303.585692E-430.8333347
AAGCGGG303.585692E-430.8333346
GCCTTAT551.8346327E-830.27272826
GGTATCA3550.028.6619721
CCTTATT604.2738975E-827.75000227
ATACGGC556.204991E-726.9090929
CGATAAA358.8357466E-426.428571
CTCACAA358.8357466E-426.4285732
TCACAAT358.8357466E-426.4285733
TACGGCT601.3253575E-624.66666830
GTCCTGT451.3162327E-424.66666637
TGGGCAG1250.023.686
AGCCCGC400.001923930423.12518
AACCCTG400.001923930423.12536
GTGAAAA400.001923930423.12512
GCCCGCT400.001923930423.12519
AAAGCCC400.001923930423.12516
Produced by FastQC (version 0.11.5)