FastQCFastQC Report
Fri 10 Feb 2017
ERR1631832.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631832.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890837
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA16870.189372466567958No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15770.17702452861746873No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA15720.17646325871062832No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA15520.17421817908326662No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15370.17253436936274538No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA14680.1647888446483476No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12600.14144001652378607No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA12550.14087874661694563No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12440.1396439528218967No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC12160.13650084134359036No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12100.13582731745538185No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT11600.13021461838697762No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC10230.1148358229395501No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10110.11348877516313309No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT10090.11326426720039692No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT9730.1092231238711459No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA9420.1057432504487353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC950.031.15789629
GGTATCA12500.027.5281
CGTTATT400.001930848223.12510
TGTTACG400.001930848223.12516
CCGCTTA705.099797E-621.14285725
TTAGGAC1600.020.81253
TACGGCT1600.020.812530
TAGCGAA450.003825141820.55555710
TAGCTAG450.003825141820.5555575
TTAACGG1101.7535058E-920.18181835
TACACTT555.141846E-420.1818185
GCGGTAA5450.019.68807423
CACATGT1650.019.06060628
GTTCTAA701.2188066E-418.51
TTTACCG701.2188066E-418.530
GTTTAAC1501.2732926E-1118.533
TTACCGG701.2188066E-418.531
CCGTTTA701.2188066E-418.527
GTTATTC500.0070336718.53
CTTATAC9350.018.20320937
Produced by FastQC (version 0.11.5)