##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631832.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	890837
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61953982602878	34.0	31.0	34.0	31.0	34.0
2	32.78629760551032	34.0	31.0	34.0	31.0	34.0
3	32.88348934765844	34.0	31.0	34.0	31.0	34.0
4	36.30001672584322	37.0	37.0	37.0	35.0	37.0
5	36.21652445958127	37.0	35.0	37.0	35.0	37.0
6	36.2651012474785	37.0	37.0	37.0	35.0	37.0
7	36.2554687333373	37.0	37.0	37.0	35.0	37.0
8	36.236953561650445	37.0	37.0	37.0	35.0	37.0
9	38.06597278738984	39.0	38.0	39.0	37.0	39.0
10	37.99544136581664	39.0	38.0	39.0	35.0	39.0
11	38.06982983418965	39.0	38.0	39.0	37.0	39.0
12	38.003907561091424	39.0	38.0	39.0	35.0	39.0
13	38.06403528367142	39.0	38.0	39.0	35.0	39.0
14	39.46668357959986	40.0	39.0	41.0	37.0	41.0
15	39.46555991724637	41.0	39.0	41.0	37.0	41.0
16	39.42076159836199	40.0	39.0	41.0	37.0	41.0
17	39.385811321263034	40.0	39.0	41.0	37.0	41.0
18	39.407041916759184	41.0	39.0	41.0	37.0	41.0
19	39.4324303997252	41.0	39.0	41.0	37.0	41.0
20	39.41729070525809	41.0	39.0	41.0	37.0	41.0
21	39.38838979521506	40.0	39.0	41.0	37.0	41.0
22	39.36139271269604	40.0	39.0	41.0	36.0	41.0
23	39.30184646573952	40.0	39.0	41.0	36.0	41.0
24	39.31532031112314	40.0	39.0	41.0	36.0	41.0
25	39.267970459242264	40.0	39.0	41.0	36.0	41.0
26	39.20265660272306	40.0	39.0	41.0	36.0	41.0
27	39.10001717485915	40.0	39.0	41.0	36.0	41.0
28	39.093032732138425	40.0	39.0	41.0	35.0	41.0
29	39.05879302274153	40.0	39.0	41.0	35.0	41.0
30	39.01936830194525	40.0	39.0	41.0	35.0	41.0
31	38.93444704250048	40.0	38.0	41.0	35.0	41.0
32	38.88928277563684	40.0	38.0	41.0	35.0	41.0
33	38.824872563667654	40.0	38.0	41.0	35.0	41.0
34	38.79093818510008	40.0	38.0	41.0	35.0	41.0
35	38.723396087050716	40.0	38.0	41.0	35.0	41.0
36	38.6803253569396	40.0	38.0	41.0	35.0	41.0
37	38.628650359156616	40.0	38.0	41.0	35.0	41.0
38	38.59041216294339	40.0	38.0	41.0	35.0	41.0
39	38.52146352250748	40.0	38.0	41.0	35.0	41.0
40	38.19866485114561	40.0	38.0	41.0	34.0	41.0
41	38.25903616486518	40.0	38.0	41.0	34.0	41.0
42	38.23611502440963	40.0	37.0	41.0	34.0	41.0
43	37.763882730510744	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	1.0
13	1.0
14	1.0
15	1.0
16	0.0
17	1.0
18	3.0
19	9.0
20	28.0
21	60.0
22	126.0
23	207.0
24	411.0
25	689.0
26	1149.0
27	1813.0
28	2877.0
29	4204.0
30	5910.0
31	8049.0
32	11177.0
33	15231.0
34	22150.0
35	33248.0
36	51294.0
37	92787.0
38	209615.0
39	429793.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.97542648093871	16.866385208517386	12.638563508251229	27.519624802292675
2	20.296979133107403	19.52006932805889	33.28992846053767	26.89302307829603
3	21.030558901347835	20.432581942600052	28.89361353423803	29.643245621814092
4	15.192790600300615	16.14526563220881	34.09972868212704	34.56221508536354
5	16.85740489000794	33.03690798653401	33.373894438601	16.731792684857048
6	33.900702373161415	34.003190258150475	15.56985172371601	16.52625564497209
7	30.46775111496267	27.983570507286963	20.25252655648564	21.29615182126472
8	27.23562222943142	31.277439082570663	19.928898328201456	21.55804035979646
9	28.18259681625258	13.262583390676408	18.190420918753937	40.364398874317075
10	19.211932149203502	23.863961645059646	30.13851018761008	26.78559601812677
11	38.3574099414371	20.103004253303354	19.398386012255887	22.141199793003658
12	22.840654350908192	24.145494630330802	27.042769889441054	25.971081129319955
13	34.057521185132636	18.20198307883485	21.394935324868637	26.34556041116388
14	23.81221255964896	20.14835486177606	23.779097635145373	32.260334943429605
15	28.74150938948427	24.70957088670542	20.589063992627157	25.959855731183147
16	25.233460217750274	24.441171617254334	23.086939586029768	27.238428578965625
17	26.048424122482565	24.253033944481427	22.717287225384666	26.981254707651342
18	26.206140966304726	22.3105910508881	23.99731937492493	27.48594860788225
19	27.43498530034114	22.969409667537384	24.112604213789954	25.483000818331526
20	28.54293209644413	21.89603709769576	23.67896708376504	25.88206372209506
21	26.81983348244404	23.104226699160453	23.07762250557622	26.998317312819292
22	27.377623515862048	23.466245789072524	22.604471974109742	26.551658720955686
23	27.0914881173548	22.84604254201386	23.45838801037676	26.60408133025458
24	26.93983298852652	22.570795779699317	23.47129721823409	27.018074013540076
25	27.143349456746858	22.786435677907406	23.805589574748243	26.264625290597493
26	26.94499667166945	23.502054809128943	23.318968565517597	26.233979953684006
27	26.429750897189948	22.695397699017892	24.39189211943375	26.482959284358422
28	25.924944742977672	23.55604897416699	23.193244106385343	27.325762176469993
29	26.536953449396467	23.861828819413653	23.387892510077602	26.21332522111228
30	26.308628851293786	22.743779164987536	24.389871547769122	26.557720435949562
31	26.995510963285092	23.088174379824817	23.50980033384334	26.406514323046753
32	25.1693631943891	23.1237588919185	23.80098716151215	27.90589075218025
33	25.267697682067542	22.650720614433393	25.271402063452687	26.810179640046382
34	25.814262317348742	22.579551590246027	24.74504314481774	26.861142947587496
35	25.3044047339749	23.76697420515762	24.910393259372928	26.018227801494547
36	25.628369724203193	22.577082002655928	25.347847024764352	26.446701248376524
37	25.776993995534536	22.275006538794415	25.296659209260504	26.651340256410545
38	25.076753659760424	21.79063060919113	26.16101486579475	26.971600865253688
39	24.690263201910113	21.05166264984503	26.989449248291216	27.268624899953643
40	24.496288322106064	21.96013412105694	27.487520163621404	26.056057393215593
41	23.156649308459347	21.374392846278276	28.347161152938195	27.121796692324185
42	21.876953920863187	22.0759802298288	28.934249475493274	27.11281637381474
43	21.457909808416133	22.437774811778137	28.622969185159576	27.48134619464616
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	10.5
2	14.0
3	44.0
4	74.0
5	74.0
6	96.5
7	119.0
8	130.5
9	142.0
10	256.0
11	370.0
12	370.0
13	704.0
14	1038.0
15	1935.5
16	2833.0
17	2812.0
18	2791.0
19	2791.0
20	3219.0
21	3647.0
22	3017.5
23	2388.0
24	2550.0
25	2712.0
26	2712.0
27	3253.0
28	3794.0
29	4840.0
30	5886.0
31	7470.5
32	9055.0
33	9055.0
34	11726.5
35	14398.0
36	16655.5
37	18913.0
38	22890.0
39	26867.0
40	26867.0
41	31343.0
42	35819.0
43	41484.5
44	47150.0
45	57998.5
46	68847.0
47	68847.0
48	74951.0
49	81055.0
50	89350.0
51	97645.0
52	95938.5
53	94232.0
54	94232.0
55	86575.5
56	78919.0
57	73997.5
58	69076.0
59	65090.5
60	61105.0
61	61105.0
62	55789.5
63	50474.0
64	42459.0
65	34444.0
66	30076.0
67	25708.0
68	25708.0
69	21993.5
70	18279.0
71	15357.0
72	12435.0
73	10093.0
74	7751.0
75	7751.0
76	6422.5
77	5094.0
78	4156.5
79	3219.0
80	2612.5
81	2006.0
82	2006.0
83	1572.0
84	1138.0
85	932.5
86	727.0
87	559.5
88	392.0
89	392.0
90	300.0
91	208.0
92	132.0
93	56.0
94	32.0
95	8.0
96	8.0
97	4.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	890837.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.32166453573367
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.63960043703437	46.19767710013551
2	6.8945607376538804	7.352589095488414
3	2.1377674443277996	3.4196795556557893
4	1.0592429311181801	2.259223849397234
5	0.6319562440098191	1.6848479422176912
6	0.42439282383322924	1.35775990702849
7	0.2896889524704742	1.0812688000337105
8	0.23234827881017284	0.991135758253692
9	0.18315901782648458	0.8789709334714447
>10	1.239280967068477	12.965510985684991
>50	0.14752897455085393	5.451866863860593
>100	0.10863722864743575	11.113565883483934
>500	0.008665615510759457	3.016608539743957
>1k	0.003170347138082729	2.2292947855447083
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1687	0.189372466567958	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1577	0.17702452861746873	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1572	0.17646325871062832	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1552	0.17421817908326662	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1537	0.17253436936274538	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1468	0.1647888446483476	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1260	0.14144001652378607	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1255	0.14087874661694563	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1244	0.1396439528218967	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1216	0.13650084134359036	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1210	0.13582731745538185	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1160	0.13021461838697762	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1023	0.1148358229395501	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1011	0.11348877516313309	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1009	0.11326426720039692	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	973	0.1092231238711459	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	942	0.1057432504487353	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.1225398136808418E-4	0.0	0.0	0.0	0.0
7	1.1225398136808418E-4	0.0	0.0	0.0	0.0
8	1.1225398136808418E-4	0.0	0.0	0.0	0.0
9	1.1225398136808418E-4	0.0	0.0	0.0	0.0
10	2.2450796273616836E-4	0.0	0.0	1.1225398136808418E-4	1.1225398136808418E-4
11	2.2450796273616836E-4	0.0	0.0	1.1225398136808418E-4	1.1225398136808418E-4
12	2.2450796273616836E-4	0.0	0.0	1.1225398136808418E-4	1.1225398136808418E-4
13	2.2450796273616836E-4	0.0	0.0	1.1225398136808418E-4	1.1225398136808418E-4
14	2.2450796273616836E-4	0.0	0.0	2.2450796273616836E-4	1.1225398136808418E-4
15	2.2450796273616836E-4	0.0	0.0	3.367619441042525E-4	1.1225398136808418E-4
16	2.2450796273616836E-4	0.0	0.0	6.73523888208505E-4	1.1225398136808418E-4
17	2.2450796273616836E-4	0.0	0.0	0.0011225398136808418	1.1225398136808418E-4
18	2.2450796273616836E-4	0.0	0.0	0.0015715557391531784	1.1225398136808418E-4
19	2.2450796273616836E-4	0.0	0.0	0.00269409555283402	1.1225398136808418E-4
20	2.2450796273616836E-4	0.0	0.0	0.0035921274037786937	1.1225398136808418E-4
21	2.2450796273616836E-4	0.0	0.0	0.005612699068404209	1.1225398136808418E-4
22	2.2450796273616836E-4	0.0	0.0	0.008082286658502061	1.1225398136808418E-4
23	2.2450796273616836E-4	0.0	0.0	0.00965384239765524	2.2450796273616836E-4
24	2.2450796273616836E-4	0.0	0.0	0.012235683969121175	2.2450796273616836E-4
25	2.2450796273616836E-4	0.0	0.0	0.014031747671010522	2.2450796273616836E-4
26	2.2450796273616836E-4	0.0	0.0	0.01829739896299772	2.2450796273616836E-4
27	2.2450796273616836E-4	0.0	0.0	0.03928889347882946	2.2450796273616836E-4
28	2.2450796273616836E-4	0.0	0.0	0.12942884051740106	2.2450796273616836E-4
29	2.2450796273616836E-4	0.0	0.0	0.27962466758789767	2.2450796273616836E-4
30	2.2450796273616836E-4	0.0	0.0	0.46776234036080677	2.2450796273616836E-4
31	2.2450796273616836E-4	0.0	0.0	1.0260013897042894	2.2450796273616836E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGGC	95	0.0	31.157896	29
GGTATCA	1250	0.0	27.528	1
CGTTATT	40	0.0019308482	23.125	10
TGTTACG	40	0.0019308482	23.125	16
CCGCTTA	70	5.099797E-6	21.142857	25
TTAGGAC	160	0.0	20.8125	3
TACGGCT	160	0.0	20.8125	30
TAGCGAA	45	0.0038251418	20.555557	10
TAGCTAG	45	0.0038251418	20.555557	5
TTAACGG	110	1.7535058E-9	20.181818	35
TACACTT	55	5.141846E-4	20.181818	5
GCGGTAA	545	0.0	19.688074	23
CACATGT	165	0.0	19.060606	28
GTTCTAA	70	1.2188066E-4	18.5	1
TTTACCG	70	1.2188066E-4	18.5	30
GTTTAAC	150	1.2732926E-11	18.5	33
TTACCGG	70	1.2188066E-4	18.5	31
CCGTTTA	70	1.2188066E-4	18.5	27
GTTATTC	50	0.00703367	18.5	3
CTTATAC	935	0.0	18.203209	37
>>END_MODULE