FastQCFastQC Report
Fri 10 Feb 2017
ERR1631831.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631831.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470442
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA13160.2797369282504539No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA12700.269958889724982No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA11830.25146564294854623No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA11810.2510405108387431No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC10470.222556659481934No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA10420.2214938292074262No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT10310.21915560260350903No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG9980.21214092279175753No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC8650.18386963748984997No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8580.18238167510553904No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT8260.1755795613486891No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7940.16877744759183916No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA7740.16452612649380796No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6680.14199412467424252No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6650.14135642650953784No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6640.14114386045463628No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6320.13434174669778634No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6300.13391661458798323No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA6230.13242865220367228No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT5990.12732706688603485No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5690.12095008523898801No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA5490.1166987641409568No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT5470.11627363203115368No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5460.11606106597625211No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5300.11266000909782715No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5210.1107469146037131No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA5210.1107469146037131No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA5120.10883382010959906No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG5050.10734585772528814No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC4980.1058578953409772No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4980.1058578953409772No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4920.10458249901156784No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC4860.10330710268215848No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT4850.10309453662725693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8450.028.023671
ATACGGC706.5720087E-926.4285729
TACGGCT701.917906E-723.78571330
TGTTACG400.001929500523.12500216
AGGGGTT400.001929500523.1250025
GCTAAAC603.720264E-521.5833343
GCGGTAA4700.021.2553223
TATTAGA705.091357E-621.1428572
GACAGGG450.003822499420.5555557
CTACACT450.003822499420.5555554
TTATTGT450.003822499420.55555512
GTAACAC450.003822499420.5555553
AATACTG555.136887E-420.1818185
CTAAACC951.6708691E-719.4736844
CGTGCCA5250.019.38095310
TAAACCA1052.2509994E-819.3809535
AGCCGCG5200.019.21153819
CGCGGTA5250.019.02857222
AACTCCG5350.019.0186925
GTATCAA12500.018.7962
Produced by FastQC (version 0.11.5)