FastQCFastQC Report
Fri 10 Feb 2017
ERR1631830.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631830.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2981537
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT184330.6182381771549372No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT180190.6043527214319326No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT150980.5063831171640667No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50500.1693757280221577No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA46800.15696602121657385No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA45160.15146550252436913No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG39980.1340919129965518No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA38330.12855785455622384No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA36100.12107849072475035No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT35380.11866362885987998No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA34680.11631584649125601No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC34140.11450470009260325No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC33220.11141904326526887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64950.026.2902221
CTTATAC42400.018.84905637
ATACACA10100.017.76732637
CAGTCGG11200.017.17857210
CTCTATG19550.016.938621
GTATCAA101200.016.745062
GCAGTCG11700.016.2863259
TCTATGG20850.016.237412
AGTCGGT11950.016.10041811
TCTTATA72700.015.70082537
TTACGAG1557.221388E-915.51612956
TATTAGA4550.015.4505492
GCGGTAA11500.015.44347923
TTCGCTA2400.015.41666626
TATACAC13500.014.937036537
TTTTACG1508.114512E-814.84
TCGCTAC2800.014.53571527
TGCGGGT12300.014.28861821
TTCTGCG12700.014.27559118
TATGGGC22850.014.2494534
Produced by FastQC (version 0.11.5)