FastQCFastQC Report
Fri 10 Feb 2017
ERR1631829.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631829.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529392
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA13650.25784295947048685No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA12650.2389533653700849No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA12360.23347538308096832No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA11230.21213014174751413No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA10020.18927373288602772No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9730.18379575059691117No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG9640.182095687127875No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT9600.18134010336385892No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC8380.15829479856136852No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT8370.15810590262036447No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA8350.15772811073835646No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7400.13978299634297459No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7160.1352494937588781No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA5670.10710399854927917No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5550.10483724725723093No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5540.10464835131622692No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5310.10030374467313447No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATA200.001841039537.09
CTACACT405.9341703E-527.754
GGTATCA6650.026.9849621
TAGCGAA358.863047E-426.42857210
TAAAAGT358.863047E-426.4285724
TATGCGT358.863047E-426.4285723
CTAAGTC603.7214E-521.58333434
CTTATAC3450.021.44927637
AAGTAAG450.003823122520.5555557
GTAGCGA450.003823122520.5555559
GTAAGAG450.003823122520.5555559
TCTTATA7250.020.1586237
TAAGTCT656.8931426E-519.92307735
GTGTCAA759.250298E-619.73333228
TTCTGCG3000.019.73333218
ATAACGC851.2427136E-619.58823635
GGACCGT1052.2524546E-819.380956
TGCGGGT3100.019.09677321
GTAACGT3100.019.09677326
GTATCAA9400.019.0904252
Produced by FastQC (version 0.11.5)