FastQCFastQC Report
Fri 10 Feb 2017
ERR1631828.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631828.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019466
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34540.3388048252712695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32830.32203133797497907No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25930.2543488453759125No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA19110.18745107732871916No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA16520.16204561996182315No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA15990.15684681980566295No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA15440.15145183851153446No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15440.15145183851153446No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG14050.137817249422737No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA13550.13291272097352927No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT12570.12329984521308214No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA11830.11604114310825471No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC11650.11427551286653993No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC11590.11368696945263501No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT11550.1132946071766984No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA10600.10397600312320372No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC10590.10387791255421956No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGCA250.005496056329.59999810
GGTATCA17150.028.262391
TTAGACT502.7018125E-422.1999994
TCTATCG450.003825515720.55555531
CCGCTTA759.264084E-619.73333425
TAGAGTG701.2190258E-418.55
GTATCAA26200.018.52
CTAGACC500.00703435118.4999983
CTTATAC11950.017.95815837
TATACAC3600.017.47222137
GTTTAGG852.722627E-517.4117641
ATACGGC852.722627E-517.41176429
TACGGCT752.0669772E-417.26666630
CATAACG752.0669772E-417.26666612
GCAGTCG3350.017.1194049
ATAGGAC650.001579768717.0769233
GCGGTAA6500.017.07692323
ATAACGT650.001579768717.07692313
AGTCGGT3400.016.86764711
CTCTATG4950.016.818181
Produced by FastQC (version 0.11.5)