##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631828.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1019466
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.447778542884215	33.0	31.0	34.0	31.0	34.0
2	32.622229677105466	34.0	31.0	34.0	31.0	34.0
3	32.708464039016505	34.0	31.0	34.0	31.0	34.0
4	36.19222220260411	37.0	35.0	37.0	35.0	37.0
5	36.08301404853129	37.0	35.0	37.0	35.0	37.0
6	36.13151787308257	37.0	35.0	37.0	35.0	37.0
7	36.125642248000425	37.0	35.0	37.0	35.0	37.0
8	36.10729538797763	37.0	35.0	37.0	35.0	37.0
9	37.90092165898618	39.0	38.0	39.0	35.0	39.0
10	37.81309332532914	39.0	38.0	39.0	35.0	39.0
11	37.88716445668615	39.0	38.0	39.0	35.0	39.0
12	37.82462190990185	39.0	38.0	39.0	35.0	39.0
13	37.88064437656577	39.0	38.0	39.0	35.0	39.0
14	39.23349773312695	40.0	39.0	41.0	36.0	41.0
15	39.225097256799145	40.0	39.0	41.0	36.0	41.0
16	39.17690339844585	40.0	39.0	41.0	36.0	41.0
17	39.14522504919242	40.0	39.0	41.0	36.0	41.0
18	39.17436579542623	40.0	39.0	41.0	36.0	41.0
19	39.19868539019448	40.0	39.0	41.0	36.0	41.0
20	39.18608859932553	40.0	39.0	41.0	36.0	41.0
21	39.156398545905404	40.0	39.0	41.0	36.0	41.0
22	39.12871640643239	40.0	39.0	41.0	36.0	41.0
23	39.064883968665946	40.0	39.0	41.0	36.0	41.0
24	39.07626836010225	40.0	39.0	41.0	36.0	41.0
25	39.02404886479785	40.0	39.0	41.0	35.0	41.0
26	38.94590501301662	40.0	38.0	41.0	35.0	41.0
27	38.83231024869883	40.0	38.0	41.0	35.0	41.0
28	38.8182734882772	40.0	38.0	41.0	35.0	41.0
29	38.785221871057985	40.0	38.0	41.0	35.0	41.0
30	38.72457541497215	40.0	38.0	41.0	35.0	41.0
31	38.62938538411286	40.0	38.0	41.0	35.0	41.0
32	38.56354601330501	40.0	38.0	41.0	35.0	41.0
33	38.49491204218679	40.0	38.0	41.0	35.0	41.0
34	38.450690851877354	40.0	38.0	41.0	35.0	41.0
35	38.3712168919807	40.0	38.0	41.0	34.0	41.0
36	38.31500314870726	40.0	38.0	41.0	34.0	41.0
37	38.25784086963175	40.0	38.0	41.0	34.0	41.0
38	38.21376681517579	40.0	38.0	41.0	34.0	41.0
39	38.125642248000425	40.0	37.0	41.0	34.0	41.0
40	37.80637019773097	40.0	37.0	41.0	33.0	41.0
41	37.85544294758236	40.0	37.0	41.0	33.0	41.0
42	37.817927228568685	40.0	37.0	41.0	33.0	41.0
43	37.319313248308426	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	0.0
15	1.0
16	4.0
17	8.0
18	7.0
19	25.0
20	54.0
21	95.0
22	242.0
23	378.0
24	733.0
25	1102.0
26	1871.0
27	2862.0
28	4251.0
29	6130.0
30	8517.0
31	11336.0
32	15250.0
33	20309.0
34	29135.0
35	43199.0
36	67153.0
37	124528.0
38	275522.0
39	406752.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.615349604596915	17.327110467636977	13.293135818163627	26.76440410960248
2	19.9739863811054	20.398816635375773	33.85095726586272	25.776239717656107
3	21.065145870485136	20.962837407034662	29.753321837118644	28.21869488536155
4	15.275840489040341	16.827535199800682	34.2812805919962	33.61534371916278
5	16.41712425917098	33.5681621554814	33.52264813147275	16.492065453874872
6	32.80452707593975	34.318162645934244	16.178273723694563	16.699036554431437
7	29.281113838028926	28.850103877912552	20.75822048013372	21.1105618039248
8	26.664057457531687	31.877080746194576	20.391950295546884	21.06691150072685
9	27.837711115427094	13.841364008216066	18.726862887040863	39.594061989315975
10	18.919316583387776	24.568450541754213	30.525294615023945	25.98693825983407
11	37.206635630810645	20.82109653485256	20.08443636178156	21.887831472555238
12	22.12933045339423	24.79062568050332	27.7924913631254	25.28755250297705
13	33.57081060084397	18.674482523203324	22.38103085340757	25.373676022545137
14	23.415395903345477	21.030421809064745	24.659478589771506	30.894703697818272
15	28.228307761122	25.416345420053243	21.451426531144737	24.90392028768002
16	24.376879660528157	25.203390794788643	23.863277441327128	26.556452103356072
17	25.21879101411916	24.878809102020078	23.663761224013356	26.238638659847414
18	25.368281041251006	22.814002624903626	25.183380318715876	26.634336015129488
19	26.706432583332845	23.556352050975708	25.45754345902659	24.279671906664863
20	27.877143524158726	22.36425736611128	24.831823719476667	24.92677539025333
21	25.990077158041565	23.703193632744988	24.074074074074073	26.232655135139378
22	26.402449910050947	23.99138372442043	23.79706630726282	25.8091000582658
23	26.168994355868662	23.52672869914249	24.562859379322116	25.74141756566673
24	26.19439981323556	23.199498561011353	24.874198845277824	25.73190278047527
25	26.21597973841207	23.425008779105923	25.007896290803224	25.35111519167878
26	26.04716586919034	24.029050502910348	24.520091891245023	25.403691736654288
27	25.688448658415286	23.251192290866	25.67383316363665	25.386525887082062
28	25.059590020657872	24.150388536743748	24.32803055717405	26.46199088542433
29	25.581628028791542	24.642607011906232	24.345392587884245	25.430372371417974
30	25.451756115456526	23.830417100717437	25.339932866814586	25.377893917011455
31	26.27983669882076	23.599217629621783	24.68880766989777	25.432138001659695
32	24.182562243370548	23.70898097631505	25.176612069455974	26.93184471085843
33	24.52568305367712	23.374492136079084	26.303770797652888	25.796054012590908
34	24.900290936627606	23.28130609554414	25.754071249065685	26.064331718762567
35	24.64084138166452	24.246713475486185	25.943484137774092	25.168961005075207
36	24.736185414717117	23.35310839204054	26.299258631479617	25.61144756176273
37	25.23988048645075	22.912976009008638	26.032648464980685	25.814495039559926
38	24.340389968866052	22.489519022704044	26.8967282871621	26.2733627212678
39	23.959700470638552	21.80661248143636	27.95237899056957	26.281308057355517
40	23.82727820250994	22.555926337906314	28.244688886142356	25.37210657344139
41	22.40182605403221	22.21054944451311	29.13741115446714	26.25021334698754
42	21.48664104541005	22.61693867181446	29.38548220342807	26.510938079347422
43	20.896135820125437	23.203520274339702	29.048639189536484	26.851704715998377
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	34.0
1	37.0
2	40.0
3	86.0
4	132.0
5	132.0
6	217.0
7	302.0
8	334.0
9	366.0
10	565.5
11	765.0
12	765.0
13	1559.5
14	2354.0
15	4399.0
16	6444.0
17	6277.0
18	6110.0
19	6110.0
20	7046.0
21	7982.0
22	6864.5
23	5747.0
24	6106.0
25	6465.0
26	6465.0
27	7086.5
28	7708.0
29	9661.0
30	11614.0
31	13111.5
32	14609.0
33	14609.0
34	17263.0
35	19917.0
36	21931.5
37	23946.0
38	28669.0
39	33392.0
40	33392.0
41	38887.0
42	44382.0
43	48776.0
44	53170.0
45	66119.5
46	79069.0
47	79069.0
48	86406.5
49	93744.0
50	102674.0
51	111604.0
52	110937.0
53	110270.0
54	110270.0
55	98732.5
56	87195.0
57	80290.0
58	73385.0
59	66741.5
60	60098.0
61	60098.0
62	54966.0
63	49834.0
64	41462.0
65	33090.0
66	28760.0
67	24430.0
68	24430.0
69	21111.0
70	17792.0
71	15153.5
72	12515.0
73	10271.0
74	8027.0
75	8027.0
76	6540.5
77	5054.0
78	4207.5
79	3361.0
80	2645.0
81	1929.0
82	1929.0
83	1558.5
84	1188.0
85	962.0
86	736.0
87	559.0
88	382.0
89	382.0
90	292.0
91	202.0
92	132.5
93	63.0
94	38.0
95	13.0
96	13.0
97	8.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1019466.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.29955878307729
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.93320491982578	42.85768646146255
2	6.379740340331323	6.290367678578672
3	2.027876354582816	2.99920228642704
4	1.0335743574190002	2.038190391610373
5	0.6339576947472505	1.5626917319088118
6	0.43445091874570063	1.2850943164239361
7	0.3233160005766096	1.115753532315521
8	0.24573268208983703	0.9691610244488931
9	0.18205987161088524	0.8077924208268306
>10	1.4647483069771496	14.530559858644265
>50	0.19756719969341854	6.667798083962404
>100	0.12917426190786574	12.199900142374046
>500	0.011197768816046989	3.8783137194024873
>1k	0.003399322676299979	2.7974883516141476
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3454	0.3388048252712695	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3283	0.32203133797497907	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2593	0.2543488453759125	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1911	0.18745107732871916	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1652	0.16204561996182315	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1599	0.15684681980566295	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1544	0.15145183851153446	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1544	0.15145183851153446	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1405	0.137817249422737	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1355	0.13291272097352927	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1257	0.12329984521308214	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1183	0.11604114310825471	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1165	0.11427551286653993	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1159	0.11368696945263501	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1155	0.1132946071766984	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1060	0.10397600312320372	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1059	0.10387791255421956	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.809056898415445E-5	0.0
10	0.0	0.0	0.0	2.9427170695246334E-4	0.0
11	0.0	0.0	0.0	3.923622759366178E-4	0.0
12	0.0	0.0	0.0	4.904528449207723E-4	0.0
13	0.0	0.0	0.0	5.885434139049267E-4	0.0
14	0.0	0.0	0.0	9.809056898415446E-4	0.0
15	0.0	0.0	0.0	9.809056898415446E-4	0.0
16	0.0	0.0	0.0	0.0016675396727306255	0.0
17	0.0	0.0	0.0	0.002157992517651398	0.0
18	2.9427170695246334E-4	0.0	0.0	0.002452264224603861	0.0
19	2.9427170695246334E-4	0.0	0.0	0.0031388982074929423	0.0
20	2.9427170695246334E-4	0.0	0.0	0.004119803897334487	0.0
21	3.923622759366178E-4	0.0	0.0	0.0054930718631126495	0.0
22	4.904528449207723E-4	0.0	0.0	0.008435788932637283	0.0
23	5.885434139049267E-4	0.0	0.0	0.009710966329431291	0.0
24	5.885434139049267E-4	0.0	0.0	0.012261321123019307	0.0
25	5.885434139049267E-4	0.0	0.0	0.014223132502702395	0.0
26	5.885434139049267E-4	0.0	0.0	0.017558211848163648	0.0
27	5.885434139049267E-4	0.0	0.0	0.04060949555943994	0.0
28	5.885434139049267E-4	0.0	0.0	0.13781724942273701	0.0
29	5.885434139049267E-4	0.0	0.0	0.28711109541662005	0.0
30	5.885434139049267E-4	0.0	0.0	0.47907433891861034	0.0
31	5.885434139049267E-4	0.0	0.0	1.0438798351293717	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCGCA	25	0.0054960563	29.599998	10
GGTATCA	1715	0.0	28.26239	1
TTAGACT	50	2.7018125E-4	22.199999	4
TCTATCG	45	0.0038255157	20.555555	31
CCGCTTA	75	9.264084E-6	19.733334	25
TAGAGTG	70	1.2190258E-4	18.5	5
GTATCAA	2620	0.0	18.5	2
CTAGACC	50	0.007034351	18.499998	3
CTTATAC	1195	0.0	17.958158	37
TATACAC	360	0.0	17.472221	37
GTTTAGG	85	2.722627E-5	17.411764	1
ATACGGC	85	2.722627E-5	17.411764	29
TACGGCT	75	2.0669772E-4	17.266666	30
CATAACG	75	2.0669772E-4	17.266666	12
GCAGTCG	335	0.0	17.119404	9
ATAGGAC	65	0.0015797687	17.076923	3
GCGGTAA	650	0.0	17.076923	23
ATAACGT	65	0.0015797687	17.076923	13
AGTCGGT	340	0.0	16.867647	11
CTCTATG	495	0.0	16.81818	1
>>END_MODULE