FastQCFastQC Report
Fri 10 Feb 2017
ERR1631827.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631827.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98039
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5400.5508012117626659No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4590.468181029998266No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4200.42840094248207344No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4180.4263609379940636No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4120.420240924530034No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4080.41616091555401424No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3970.4049408908699599No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3870.3947408684299106No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3530.3600607921337427No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3180.32436071359356994No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2960.30192066422546127No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2890.29478064851742675No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2820.2876406328093922No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2700.27540060588133297No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2490.25398055875722925No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2390.24378053631717989No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2360.24072052958516507No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2330.23766052285315026No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2310.23562051836514042No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2310.23562051836514042No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2300.23460051612113544No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2250.2295005049011108No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2220.22644049816909598No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2210.225420495925091No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2130.21726047797305154No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2110.21522047348504167No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA1990.2029804465569824No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1960.1999204398249676No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG1950.19890043758096268No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1940.19788043533695773No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1940.19788043533695773No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1900.193800426360938No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT1890.19278042411693305No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA1850.18870041514091332No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC1850.18870041514091332No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1800.1836004039208886No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT1700.17340038148083928No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1690.1723803792368343No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1650.16830037026081457No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA1640.16728036801680965No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1630.1662603657728047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.16218035679678497No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT1540.15708034557676026No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC1540.15708034557676026No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1530.15606034333275534No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT1510.15402033884474545No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT1490.15198033435673558No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1490.15198033435673558No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1490.15198033435673558No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1480.15096033211273063No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1470.1499403298687257No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA1470.1499403298687257No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1460.14892032762472077No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1430.14586032089270595No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1420.14484031864870103No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1410.1438203164046961No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG1400.14280031416069114No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT1390.14178031191668622No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1380.1407603096726813No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1380.1407603096726813No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1380.1407603096726813No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC1360.1387203051846714No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC1360.1387203051846714No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1350.13770030294066649No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG1340.13668030069666154No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG1340.13668030069666154No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA1320.13464029620865167No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1310.13362029396464672No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC1290.13158028947663686No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC1290.13158028947663686No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA1290.13158028947663686No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT1270.129540284988627No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA1230.12546027601260723No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG1210.12342027152459735No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG1200.12240026928059243No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG1190.12138026703658747No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT1170.11934026254857762No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC1170.11934026254857762No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1140.1162802558165628No Hit
GAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAA1130.11526025357255787No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC1110.11322024908454799No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG1110.11322024908454799No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC1090.11118024459653812No Hit
CACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCA1090.11118024459653812No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT1080.11016024235253317No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG1070.10914024010852824No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG1070.10914024010852824No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT1070.10914024010852824No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC1060.10812023786452331No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA1060.10812023786452331No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA1050.10710023562051836No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC1040.10608023337651343No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC1040.10608023337651343No Hit
CCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCAC1030.1050602311325085No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG1030.1050602311325085No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC1030.1050602311325085No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG1020.10404022888850356No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT1020.10404022888850356No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC1000.10200022440049368No Hit
CATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACT990.10098022215648875No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA990.10098022215648875No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA990.10098022215648875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2200.032.7954561
TTATACA352.3662482E-531.71428737
AACGACC303.5757135E-430.83333437
CACATCA250.00547214629.67
AAGGCTC250.00547214629.613
AGGCTCA250.00547214629.614
CAGCTGT250.00547214629.636
GCAGTCG601.3166718E-624.6666689
TGGGCAG601.3166718E-624.6666686
AACGTCA851.9208528E-923.94117528
CAGTCGG551.8797402E-523.54545410
TAAAGAC551.8797402E-523.54545437
CTCTATG802.6619091E-823.1251
GGGCAGT652.641511E-622.769237
GGGTAAC1005.16593E-1022.224
TTCTGCG1005.16593E-1022.218
CTGCGGG1005.16593E-1022.220
TCTTCTG1005.16593E-1022.216
GTAACGT1005.16593E-1022.226
TGCGGGT1005.16593E-1022.221
Produced by FastQC (version 0.11.5)