FastQCFastQC Report
Fri 10 Feb 2017
ERR1631826.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631826.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209861
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4680.22300475076360068No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2910.1386632104107004No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2530.12055598705810038No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA2510.11960297530270035No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2450.11674394003650035No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2430.11579092828110035No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2310.11007285774870033No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC2160.10292526958320032No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCATAA251.2301894E-437.01
AATCCTA250.005486236429.620
TACTGTT250.005486236429.67
ACTCCTA250.005486236429.633
GGTATCA1650.029.1515141
CTTGAAT358.8451547E-426.4285723
TATACAC1002.0008883E-1124.05000137
TAATACG806.904502E-720.812527
GCCCCTA450.003815550620.55555527
ACCATTG450.003815550620.5555558
GCACATA450.003815550620.55555531
ATTGCCG450.003815550620.55555511
TCAAGAT555.123864E-420.1818183
TTATACA1202.3464963E-1020.04166636
TGTCTGG656.8678266E-519.92307710
GCGGTAA1052.2331733E-819.3809523
CCGTACA1355.4569682E-1119.18518617
CCCCTAC500.00701620118.528
GGTAATA1103.8071448E-818.525
CGCGGTA1103.8071448E-818.522