FastQCFastQC Report
Fri 10 Feb 2017
ERR1631825.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631825.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223089
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9910.4442173303031526No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9400.42135649897574506No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8480.38011735226748067No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8330.37339357834765496No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7650.3429124699111117No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7510.33663694758594104No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7490.335740444396631No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7300.32722366409818504No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC6980.3128796130692235No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6850.3070523423387079No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6790.3043628327707776No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6480.2904670333364711No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA6040.27074396317164895No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5830.26133067968389295No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5640.25281389938544707No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5130.2299530680580396No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5030.22547055211148914No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4930.22098803616493867No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4930.22098803616493867No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4850.21740202340769826No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC4820.21605726862373312No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4490.20126496600011654No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA4470.20036846281080647No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC4380.19633419845891104No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4380.19633419845891104No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT4360.19543769526960092No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4300.19274818570167063No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4280.19185168251236054No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4250.1905069277283954No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4210.1887139213497752No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT4060.18199014742994948No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA4040.1810936442406394No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC4020.18019714105132928No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4020.18019714105132928No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA3870.17347336713150358No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3760.16854259959029805No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG3740.16764609640098793No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3730.1671978448063329No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3630.1627153288597824No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3540.158681064507887No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3530.15823281291323193No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG3500.1568880581292668No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG3490.15643980653461176No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT3410.15285379377737138No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC3360.15061253580409614No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC3320.14881952942547594No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA3280.14702652304685573No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC3280.14702652304685573No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3210.1438887618842704No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA3180.14254400710030526No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA3150.14119925231634012No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA3030.13582023318047953No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC2980.1335789752072043No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT2960.1326824720178942No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT2940.13178596882858412No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT2880.12909645926065383No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC2880.12909645926065383No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2860.12819995607134374No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC2850.12775170447668868No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC2800.12551044650341345No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2790.1250621949087584No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC2770.1241656917194483No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC2740.12282093693548315No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2730.1223726853408281No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG2650.11878667258358772No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT2610.11699366620496751No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG2610.11699366620496751No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT2610.11699366620496751No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG2570.11520065982634733No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT2550.11430415663703723No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC2540.11385590504238219No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT2530.11340765344772714No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC2530.11340765344772714No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA2530.11340765344772714No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT2500.112062898663762No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2470.11071814387979685No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC2470.11071814387979685No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA2470.11071814387979685No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT2460.1102698922851418No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC2450.10982164069048676No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC2420.10847688590652162No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG2400.10758038271721153No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC2370.10623562793324638No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2360.10578737633859132No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG2320.10399436995997113No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG2320.10399436995997113No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA2300.10309786677066103No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT2290.10264961517600599No Hit
ACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA2260.10130486039204084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC200.001838757737.029
GCCTAGT401.5924252E-632.3751
CCTAGTT401.5924252E-632.3752
CAATGCT303.5902648E-430.83333433
TCAATGC303.5902648E-430.83333432
GGTATCA5900.029.7881361
AAACATT250.005486968429.613
GCAATCC250.005486968429.633
TAGTTCC453.9892457E-628.7777774
CTAGTTC453.9892457E-628.7777773
GTGAGTA556.2237996E-726.909092
TGAGTAA556.2237996E-726.909093
GTTCCTT509.04827E-625.9000026
TAATGTC601.3293484E-624.6666687
AGTAATG601.3293484E-624.6666685
GTAATGT601.3293484E-624.6666686
TATACAC701.9085019E-723.78571337
TTCCTTC551.8937611E-523.5454547
TTGTAGC400.001926337523.1250022
GTCGTAA400.001926337523.12500224
Produced by FastQC (version 0.11.5)