FastQCFastQC Report
Fri 10 Feb 2017
ERR1631824.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631824.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892805
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22950.25705501201270153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21890.24518231864740903No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17540.19645947323323684No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA14970.16767379214946151No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA14600.16352955012572734No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA14220.15927330156081115No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA13320.1491927128544307No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13070.14639254932488058No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA12360.13844008490095822No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12250.13720801294795615No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC11840.13261574475949395No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC10720.12007101214710938No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT10680.11962298598238137No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA9750.1092063776524549No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT9310.10427808984044667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13500.028.3666691
ATACACA1750.021.14285737
CCGCTTA806.956543E-720.812525
TTAACGG450.00382514920.55555535
TGTACCG450.00382514920.5555555
CTTATAC8350.020.16167637
ACACGAA656.899923E-519.92307717
GTATCAA19650.019.3944022
GCGGTAA5550.018.66666623
ATTAGAG1306.9485395E-1018.53
AAGACCG500.00703368218.55
TACGGCT1002.8731301E-718.530
TAGTTCC1103.8487997E-818.54
GCTCTAA500.00703368218.51
TATTAGA1409.458745E-1118.52
AACTCCG5950.018.3445385
CGCGGTA5800.018.18103422
GTTTAAC953.6045622E-617.52631633
ATACGGC852.7220134E-517.41176429
GTAACGT4700.017.31914926
Produced by FastQC (version 0.11.5)