##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631824.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	892805
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.644867580266684	34.0	31.0	34.0	31.0	34.0
2	32.810057067332735	34.0	31.0	34.0	31.0	34.0
3	32.897266480362454	34.0	31.0	34.0	31.0	34.0
4	36.310516854184286	37.0	37.0	37.0	35.0	37.0
5	36.23109637602836	37.0	35.0	37.0	35.0	37.0
6	36.282941963810686	37.0	37.0	37.0	35.0	37.0
7	36.27281881261866	37.0	37.0	37.0	35.0	37.0
8	36.256381852700194	37.0	37.0	37.0	35.0	37.0
9	38.08535122451151	39.0	38.0	39.0	37.0	39.0
10	38.02513762803748	39.0	38.0	39.0	35.0	39.0
11	38.101390561208774	39.0	38.0	39.0	37.0	39.0
12	38.037319459456434	39.0	38.0	39.0	35.0	39.0
13	38.088768544082974	39.0	38.0	39.0	37.0	39.0
14	39.483396710367884	41.0	39.0	41.0	37.0	41.0
15	39.49015742519363	41.0	39.0	41.0	37.0	41.0
16	39.445537379382955	40.0	39.0	41.0	37.0	41.0
17	39.415192567245924	40.0	39.0	41.0	37.0	41.0
18	39.4412676900331	41.0	39.0	41.0	37.0	41.0
19	39.46721624542873	41.0	39.0	41.0	37.0	41.0
20	39.464911150811204	41.0	39.0	41.0	37.0	41.0
21	39.428867445858835	41.0	39.0	41.0	37.0	41.0
22	39.40668119018151	40.0	39.0	41.0	37.0	41.0
23	39.34476957454315	40.0	39.0	41.0	36.0	41.0
24	39.36124125648938	41.0	39.0	41.0	36.0	41.0
25	39.308519777555006	40.0	39.0	41.0	36.0	41.0
26	39.24794888021461	40.0	39.0	41.0	36.0	41.0
27	39.13970911901255	40.0	39.0	41.0	36.0	41.0
28	39.130835960820114	40.0	39.0	41.0	36.0	41.0
29	39.10262935355425	40.0	39.0	41.0	35.0	41.0
30	39.05149612737384	40.0	39.0	41.0	35.0	41.0
31	38.964234071269765	40.0	38.0	41.0	35.0	41.0
32	38.91026483946663	40.0	38.0	41.0	35.0	41.0
33	38.85272035886896	40.0	38.0	41.0	35.0	41.0
34	38.81860876675198	40.0	38.0	41.0	35.0	41.0
35	38.75133427792183	40.0	38.0	41.0	35.0	41.0
36	38.69598400546592	40.0	38.0	41.0	35.0	41.0
37	38.649031983467836	40.0	38.0	41.0	35.0	41.0
38	38.607652286893554	40.0	38.0	41.0	35.0	41.0
39	38.53279831542162	40.0	38.0	41.0	35.0	41.0
40	38.21041660832993	40.0	38.0	41.0	34.0	41.0
41	38.279729616209586	40.0	38.0	41.0	34.0	41.0
42	38.25347864315276	40.0	37.0	41.0	34.0	41.0
43	37.78030140960232	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	2.0
12	0.0
13	1.0
14	0.0
15	0.0
16	0.0
17	5.0
18	5.0
19	14.0
20	35.0
21	56.0
22	123.0
23	220.0
24	387.0
25	702.0
26	1124.0
27	1812.0
28	2687.0
29	3975.0
30	5754.0
31	7879.0
32	10795.0
33	14873.0
34	21355.0
35	32741.0
36	50543.0
37	92508.0
38	208545.0
39	436662.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.67729235387346	17.056356091195727	13.094348709964661	27.172002844966148
2	19.716511444268345	20.23185354024675	33.95870318826619	26.092931827218706
3	20.66027856026792	20.88776384540857	29.85601559131053	28.595942003012976
4	15.16501363679639	16.427103342835224	34.474717323491696	33.9331656968767
5	16.260325603015215	33.66334193916925	33.76605193743315	16.31028052038239
6	33.06914723819871	34.444139537749	15.983669446295664	16.50304377775662
7	29.549005661930654	28.748046885938138	20.626004558666224	21.076942893464977
8	26.68455037774206	32.05067175923074	20.4064717379495	20.858306125077704
9	27.776950173890153	13.76291575428005	18.6480810479332	39.812053023896596
10	18.708788593253846	24.56415454662552	30.676239492386355	26.050817367734275
11	37.27409680725354	20.764444643567185	19.9719983647045	21.989460184474773
12	22.22669003869826	24.775846909459514	27.548120810255316	25.44934224158691
13	33.36730865082521	18.679778899087708	22.376666797340967	25.57624565274612
14	23.414519407933422	20.986665621272284	24.345741791320613	31.253073179473684
15	28.317605748175694	25.393562984078272	21.288635256298967	25.000196011447066
16	24.64838346559439	25.166413718561166	23.483851456925084	26.70135135891936
17	25.517890244790298	24.852459383628002	23.32547420769373	26.30417616388797
18	25.32434294162779	22.84407009369347	25.04287050363741	26.78871646104132
19	26.825566613090206	23.53347035466871	25.138972115971576	24.50199091626951
20	28.05315830444498	22.224113888251075	24.749077346117012	24.97365046118693
21	26.04544105375754	23.75053903147944	23.61165092041375	26.59236899434927
22	26.5548468030533	24.095631184861197	23.25883031569044	26.09069169639507
23	26.173128510705023	23.583313265494706	24.16137902453503	26.082179199265237
24	26.589568830819722	23.119718191542386	24.33678126802605	25.953931709611844
25	26.36555574845571	23.338466966470843	24.7149153510565	25.581061934016947
26	26.431415594670725	23.978136323161273	23.983176617514463	25.607271464653536
27	25.876871209278622	23.12061424387184	25.06829598848573	25.93421855836381
28	25.046566719496415	24.15219448815811	24.04668432636466	26.754554465980814
29	25.896920380150203	24.593948286579938	24.005017893044954	25.504113440224906
30	25.433997345444975	23.833535878495304	25.098425748063686	25.634041027996034
31	26.32523339363019	23.674934616181584	24.390432401252234	25.609399588935993
32	24.211669961525754	23.764203829503643	24.588907992226748	27.435218216743856
33	24.686577696137455	23.218507960864915	26.015535307261946	26.079379035735688
34	24.900062163630356	23.24281338030141	25.346632243323008	26.510492212745223
35	24.831738173509333	24.326924692402034	25.607607484277082	25.233729649811547
36	25.055415236249797	23.40365477343877	26.008814914791024	25.532115075520412
37	25.50848169533101	22.99908714668937	25.56168480239246	25.930746355587164
38	24.65319974686522	22.635289900930214	26.565263411383228	26.146246940821342
39	24.30362733183618	21.862892792939107	27.35244538281036	26.481034492414356
40	23.886626979015574	22.752448743006592	27.948992221145712	25.41193205683212
41	22.70327787142769	22.173710944719172	28.818274987259258	26.30473619659388
42	21.83836336042025	22.382267124400066	29.14410201555771	26.63526749962198
43	21.094751933512917	23.12218233544839	29.0564008938122	26.726664837226494
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22.0
1	22.0
2	22.0
3	63.0
4	104.0
5	104.0
6	143.5
7	183.0
8	207.5
9	232.0
10	369.0
11	506.0
12	506.0
13	971.0
14	1436.0
15	2912.0
16	4388.0
17	4297.5
18	4207.0
19	4207.0
20	4755.5
21	5304.0
22	4726.5
23	4149.0
24	4584.0
25	5019.0
26	5019.0
27	5668.0
28	6317.0
29	8397.0
30	10477.0
31	11745.0
32	13013.0
33	13013.0
34	15446.0
35	17879.0
36	19552.0
37	21225.0
38	25779.0
39	30333.0
40	30333.0
41	34962.0
42	39591.0
43	42804.0
44	46017.0
45	57274.5
46	68532.0
47	68532.0
48	74533.5
49	80535.0
50	88990.0
51	97445.0
52	98098.5
53	98752.0
54	98752.0
55	88409.5
56	78067.0
57	72244.5
58	66422.0
59	60026.5
60	53631.0
61	53631.0
62	48506.0
63	43381.0
64	36260.5
65	29140.0
66	25273.0
67	21406.0
68	21406.0
69	18359.5
70	15313.0
71	13097.0
72	10881.0
73	8942.5
74	7004.0
75	7004.0
76	5711.0
77	4418.0
78	3634.0
79	2850.0
80	2342.0
81	1834.0
82	1834.0
83	1523.5
84	1213.0
85	988.5
86	764.0
87	617.0
88	470.0
89	470.0
90	357.0
91	244.0
92	150.5
93	57.0
94	39.0
95	21.0
96	21.0
97	10.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	892805.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.55108213787357
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.47988113476482	41.122119311127456
2	6.437018425666861	6.121743837637812
3	2.091547838029478	2.9836608912429488
4	1.135904532161676	2.1605395883841085
5	0.6594837084752938	1.5679581995149083
6	0.45219051861578213	1.2901289095600021
7	0.32937128372425556	1.096337267636026
8	0.2379922481470384	0.9053431151853611
9	0.19513006031961863	0.8350780973243769
>10	1.5796774774034517	15.341666058217509
>50	0.2301431452875341	7.578856640563543
>100	0.15982103241126347	14.06545635334601
>500	0.00876056029477886	2.7873312275913342
>1k	0.0030780346981655452	2.1437805026686028
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2295	0.25705501201270153	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2189	0.24518231864740903	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1754	0.19645947323323684	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1497	0.16767379214946151	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1460	0.16352955012572734	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1422	0.15927330156081115	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1332	0.1491927128544307	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1307	0.14639254932488058	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1236	0.13844008490095822	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1225	0.13720801294795615	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1184	0.13261574475949395	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1072	0.12007101214710938	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1068	0.11962298598238137	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	975	0.1092063776524549	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	931	0.10427808984044667	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.1200654118200502E-4	0.0
3	0.0	0.0	0.0	1.1200654118200502E-4	0.0
4	0.0	0.0	0.0	1.1200654118200502E-4	0.0
5	1.1200654118200502E-4	0.0	0.0	1.1200654118200502E-4	0.0
6	1.1200654118200502E-4	0.0	0.0	1.1200654118200502E-4	0.0
7	1.1200654118200502E-4	0.0	0.0	1.1200654118200502E-4	0.0
8	2.2401308236401005E-4	0.0	0.0	1.1200654118200502E-4	0.0
9	2.2401308236401005E-4	0.0	0.0	2.2401308236401005E-4	0.0
10	2.2401308236401005E-4	0.0	0.0	2.2401308236401005E-4	0.0
11	2.2401308236401005E-4	0.0	0.0	2.2401308236401005E-4	0.0
12	2.2401308236401005E-4	0.0	0.0	2.2401308236401005E-4	0.0
13	2.2401308236401005E-4	0.0	0.0	2.2401308236401005E-4	0.0
14	2.2401308236401005E-4	0.0	0.0	5.600327059100251E-4	0.0
15	2.2401308236401005E-4	0.0	0.0	8.960523294560402E-4	0.0
16	2.2401308236401005E-4	0.0	0.0	0.0019041112000940854	0.0
17	3.3601962354601507E-4	0.0	0.0	0.0026881569883681206	0.0
18	3.3601962354601507E-4	0.0	0.0	0.0031361831530961407	0.0
19	3.3601962354601507E-4	0.0	0.0	0.004032235482552181	0.0
20	3.3601962354601507E-4	0.0	0.0	0.004816281270826216	0.0
21	3.3601962354601507E-4	0.0	0.0	0.0069444055532843114	0.0
22	4.480261647280201E-4	0.0	0.0	0.010864634494654487	0.0
23	4.480261647280201E-4	0.0	0.0	0.013104765318294588	0.0
24	4.480261647280201E-4	0.0	0.0	0.015456902683116693	0.0
25	4.480261647280201E-4	0.0	0.0	0.01713700080084677	0.0
26	4.480261647280201E-4	0.0	0.0	0.01982515778921489	0.0
27	4.480261647280201E-4	0.0	0.0	0.037746204378335695	0.0
28	5.600327059100251E-4	0.0	0.0	0.12130308410011145	0.0
29	5.600327059100251E-4	0.0	0.0	0.24619037751804707	0.0
30	5.600327059100251E-4	0.0	0.0	0.4105039734320484	0.0
31	5.600327059100251E-4	0.0	0.0	0.9012046303504124	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1350	0.0	28.366669	1
ATACACA	175	0.0	21.142857	37
CCGCTTA	80	6.956543E-7	20.8125	25
TTAACGG	45	0.003825149	20.555555	35
TGTACCG	45	0.003825149	20.555555	5
CTTATAC	835	0.0	20.161676	37
ACACGAA	65	6.899923E-5	19.923077	17
GTATCAA	1965	0.0	19.394402	2
GCGGTAA	555	0.0	18.666666	23
ATTAGAG	130	6.9485395E-10	18.5	3
AAGACCG	50	0.007033682	18.5	5
TACGGCT	100	2.8731301E-7	18.5	30
TAGTTCC	110	3.8487997E-8	18.5	4
GCTCTAA	50	0.007033682	18.5	1
TATTAGA	140	9.458745E-11	18.5	2
AACTCCG	595	0.0	18.344538	5
CGCGGTA	580	0.0	18.181034	22
GTTTAAC	95	3.6045622E-6	17.526316	33
ATACGGC	85	2.7220134E-5	17.411764	29
GTAACGT	470	0.0	17.319149	26
>>END_MODULE