FastQCFastQC Report
Fri 10 Feb 2017
ERR1631823.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631823.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183127
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11250.6143277616080643No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10480.5722804392580012No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8140.44450026484352384No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6960.3800641085148558No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6960.3800641085148558No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6760.369142726086268No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5860.31999650515762285No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC5180.2828638049004243No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5040.27521883720041285No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5030.27467276807898344No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4560.24900751937180207No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4140.22607261627176767No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3960.21624337208603867No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3850.21023661175031536No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3770.20586805877888023No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3730.20368378229316267No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3670.20040736756458633No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3560.19440060722886301No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3550.19385453810743364No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3540.19330846898600423No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3370.1840252939217046No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3360.18347922480027523No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3290.17965674095026948No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3280.1791106718288401No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3250.17747246446455192No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3220.17583425710026376No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC3190.17419604973597558No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3110.16982749676454045No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA3090.16873535852168167No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2990.16327466730738777No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2960.1616364599430996No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2940.16054432170024083No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2930.15999825257881142No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2890.15781397609309386No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG2850.1556296996073763No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG2840.15508363048594692No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2780.15180721575737058No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT2670.14580045542164727No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2650.1447083171787885No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2650.1447083171787885No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG2610.14252404069307092No Hit
GGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTAC2560.13979369508592396No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG2550.13924762596449458No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG2530.1381554877216358No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT2530.1381554877216358No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2470.13487907299305946No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA2460.13433300387163008No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC2420.13214872738591252No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA2400.13105658914305374No Hit
GGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTA2390.13051052002162433No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG2380.12996445090019496No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC2360.12887231265733617No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA2320.1266880361716186No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC2310.1261419670501892No Hit
GATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACG2300.12559589792875983No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC2270.12395769056447166No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT2270.12395769056447166No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2270.12395769056447166No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC2210.12068127583589532No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA2210.12068127583589532No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT2180.11904306847160713No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA2170.11849699935017775No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC2160.11795093022874835No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT2150.11740486110731897No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT2140.11685879198588957No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC2140.11685879198588957No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC2120.11576665374303079No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA2090.11412844637874262No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT2070.11303630813588383No Hit
ACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA2050.11194416989302505No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC2040.11139810077159568No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA1990.10866775516444871No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC1960.10702954780016055No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC1950.10648347867873115No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1940.10593740955730177No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT1940.10593740955730177No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1900.10375313307158421No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1900.10375313307158421No Hit
AAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAG1900.10375313307158421No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG1890.1032070639501548No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC1890.1032070639501548No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA1880.10266099482872543No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1850.10102278746443724No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC251.229576E-437.029
CTAATAC200.001837898337.03
TACGGCT303.5877738E-430.83333430
GCGGGCT250.005484431529.633
CTAAGTC250.005484431529.634
GCTAATA250.005484431529.62
TACCGCA250.005484431529.67
ATACCGC250.005484431529.66
GGTATCA5700.026.6140351
TAAGTCT358.8408304E-426.4285735
GTCGAAC358.8408304E-426.4285717
AACGGTA358.8408304E-426.4285721
CTTCTAA358.8408304E-426.428571
GAACGGT400.001925026523.12520
CTAAGCC400.001925026523.1254
AGTCGAA400.001925026523.12516
TTCTAAG400.001925026523.1252
AAGTCTG400.001925026523.12536
GGGCAGT652.6624475E-622.769237
CTCTATG855.121001E-821.7647061
Produced by FastQC (version 0.11.5)