FastQCFastQC Report
Fri 10 Feb 2017
ERR1631822.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631822.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275182
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA20730.1625650299329821No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA20390.15989874386558156No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA17660.138490035147924No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA16300.1278248908783217No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC15000.11763026767943714No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA13270.10406357680707537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12300.027.6747971
ACGAACG358.869931E-426.4285731
GTTATCG400.00193130223.12500211
TTCTGCG5700.021.74561318
GTACTAT705.1026473E-621.1428571
GTAACGT6000.020.3526
CTTATAC9300.020.09139837
GGTAACG6200.019.69354825
GGACCGT2550.019.5882366
TGCGGGT6400.019.36718821
TCTAATA1254.129106E-1019.242
GACCGTT1551.8189894E-1219.0967757
GCGGTAA8550.019.04093623
ATTGCCG2150.018.93023311
TAATACG8650.018.8208127
GACTAGT701.2193303E-418.51
CAATACT500.00703529618.4999984
ACGTCAA6450.018.3565929
TAACGTC6700.018.2238827
CGTCAAT6300.018.2063530
Produced by FastQC (version 0.11.5)