##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631822.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1275182
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67435079855268	34.0	31.0	34.0	31.0	34.0
2	32.83787882827706	34.0	31.0	34.0	31.0	34.0
3	32.93082556058665	34.0	31.0	34.0	31.0	34.0
4	36.339149235168	37.0	37.0	37.0	35.0	37.0
5	36.252885470466175	37.0	37.0	37.0	35.0	37.0
6	36.30273168849623	37.0	37.0	37.0	35.0	37.0
7	36.294131347525294	37.0	37.0	37.0	35.0	37.0
8	36.27392874115225	37.0	37.0	37.0	35.0	37.0
9	38.11017094030499	39.0	38.0	39.0	37.0	39.0
10	38.04483203181977	39.0	38.0	39.0	35.0	39.0
11	38.12030047475576	39.0	38.0	39.0	37.0	39.0
12	38.06182960549945	39.0	38.0	39.0	37.0	39.0
13	38.11128921204973	39.0	38.0	39.0	37.0	39.0
14	39.52793405176673	41.0	39.0	41.0	37.0	41.0
15	39.53273336668805	41.0	39.0	41.0	37.0	41.0
16	39.49241284773468	41.0	39.0	41.0	37.0	41.0
17	39.455817287257815	41.0	39.0	41.0	37.0	41.0
18	39.46958473378702	41.0	39.0	41.0	37.0	41.0
19	39.49523675836077	41.0	39.0	41.0	37.0	41.0
20	39.485877310062406	41.0	39.0	41.0	37.0	41.0
21	39.4500039994291	41.0	39.0	41.0	37.0	41.0
22	39.43414273413521	41.0	39.0	41.0	37.0	41.0
23	39.37081451902552	40.0	39.0	41.0	37.0	41.0
24	39.392970572043836	41.0	39.0	41.0	36.0	41.0
25	39.338116441417775	41.0	39.0	41.0	36.0	41.0
26	39.29296445526992	40.0	39.0	41.0	36.0	41.0
27	39.19088804578484	40.0	39.0	41.0	36.0	41.0
28	39.18226417876036	40.0	39.0	41.0	36.0	41.0
29	39.164814120651016	40.0	39.0	41.0	36.0	41.0
30	39.13268772614419	40.0	39.0	41.0	36.0	41.0
31	39.0588057234183	40.0	39.0	41.0	35.0	41.0
32	39.01527860336799	40.0	39.0	41.0	35.0	41.0
33	38.96244065552995	40.0	38.0	41.0	35.0	41.0
34	38.93501398231782	40.0	38.0	41.0	35.0	41.0
35	38.878569490472735	40.0	38.0	41.0	35.0	41.0
36	38.83275406961516	40.0	38.0	41.0	35.0	41.0
37	38.790057419254666	40.0	38.0	41.0	35.0	41.0
38	38.74854569779059	40.0	38.0	41.0	35.0	41.0
39	38.68295113952361	40.0	38.0	41.0	35.0	41.0
40	38.376655253916695	40.0	38.0	41.0	34.0	41.0
41	38.46212226960544	40.0	38.0	41.0	34.0	41.0
42	38.45848357332522	40.0	38.0	41.0	34.0	41.0
43	37.999996078991074	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	0.0
13	1.0
14	0.0
15	1.0
16	3.0
17	4.0
18	15.0
19	23.0
20	59.0
21	89.0
22	165.0
23	278.0
24	559.0
25	859.0
26	1395.0
27	2261.0
28	3265.0
29	5014.0
30	7310.0
31	10367.0
32	14541.0
33	20059.0
34	29561.0
35	45014.0
36	70344.0
37	125104.0
38	295054.0
39	643834.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.23782801200142	16.86621988076996	12.339258239216049	27.556693868012566
2	19.853322898221588	19.79027307474541	33.6882892010709	26.668114825962096
3	20.565456538752898	20.566240740537427	29.471793046012255	29.396509674697413
4	15.226140268604796	15.369570774995257	34.48629293700821	34.91799601939174
5	16.255013009907604	33.978443861346854	33.803723703753654	15.962819424991883
6	34.83675271451448	34.28443939767029	15.273976577461099	15.60483131035413
7	30.60857195286634	28.54557231830437	20.11399157139922	20.731864157430078
8	27.367387557227126	32.12427716200511	20.034081409555657	20.47425387121211
9	28.135121104281584	13.25018703212561	17.709236799139262	40.90545506445354
10	18.177091583789608	24.46286098768646	31.136888695103913	26.223158733420014
11	38.37491432595504	19.970953165901022	19.826346356833767	21.827786151310168
12	22.84536638691575	23.998770371601857	27.57629891262581	25.579564328856584
13	33.53066464238046	18.157878640068635	22.079436503965706	26.2320202135852
14	23.712144619356295	19.683543211870933	23.746884758410953	32.857427410361815
15	28.194485179370478	25.011253295608	20.824243127647662	25.970018397373867
16	25.750285057348677	24.47478085481131	22.50525807296527	27.26967601487474
17	26.15548211941511	24.189880346491716	22.641552343116512	27.01308519097666
18	25.79200459228565	22.5908929078359	24.424278259887608	27.19282423999084
19	27.50885755915626	22.916885589664847	23.984890000015685	25.58936685116321
20	28.554120117755737	21.985881231071332	23.794328966374994	25.665669684797937
21	26.699796578057093	23.19017991157341	22.783963387187082	27.326060123182415
22	27.227878059759313	23.482059815775315	22.38088366993888	26.90917845452649
23	26.814133198241507	23.246956120773348	23.33949193134784	26.599418749637305
24	27.290143681450964	22.727187177987144	23.21464700725073	26.768022133311163
25	27.27422438522501	22.935706432493557	23.576164029918868	26.213905152362564
26	27.28465426895925	23.133560542730372	22.994443146154822	26.587342042155548
27	26.59628194249919	22.634965048126464	23.98324317626817	26.785509833106175
28	25.981781424141808	23.447398096899107	23.185317860509326	27.38550261844976
29	26.586479420192568	23.903489854781512	23.12054279310718	26.389487931918737
30	26.23554912161558	23.46998310829356	23.910469250663827	26.38399851942703
31	27.183335398398036	23.277696830726907	23.25440603772638	26.28456173314868
32	25.11649317509187	23.382936710210778	23.587927056686812	27.912643058010545
33	25.57164389083284	22.89602582219636	24.75325090849777	26.779079378473035
34	25.805649703336464	22.70193588052529	24.40357533277603	27.08883908336222
35	25.65735714588192	23.97022542664498	24.368756773542913	26.003660653930183
36	25.945629721874997	23.292282983919158	24.6453447429465	26.116742551259346
37	26.686778828433905	22.792981707709174	24.031471586016742	26.488767877840182
38	25.767459076429873	22.716286773182183	25.203617993353106	26.31263615703484
39	25.367751426855143	21.889267571217285	25.69037204101062	27.052608960916952
40	25.093908163697414	22.464009059099016	26.413562926703797	26.02851985049977
41	23.967088619506864	21.81586628418532	27.254619340611775	26.962425755696053
42	23.19715930745572	22.056537811857442	27.796581193900167	26.94972168678667
43	22.572464165899454	22.370453786204635	27.809285262809546	27.247796785086365
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	11.5
2	10.0
3	41.0
4	72.0
5	72.0
6	84.5
7	97.0
8	111.5
9	126.0
10	201.0
11	276.0
12	276.0
13	514.5
14	753.0
15	1468.0
16	2183.0
17	2231.5
18	2280.0
19	2280.0
20	2728.0
21	3176.0
22	3339.5
23	3503.0
24	4387.5
25	5272.0
26	5272.0
27	6324.5
28	7377.0
29	10340.0
30	13303.0
31	15293.5
32	17284.0
33	17284.0
34	20810.0
35	24336.0
36	27409.5
37	30483.0
38	36807.0
39	43131.0
40	43131.0
41	49403.5
42	55676.0
43	60341.0
44	65006.0
45	77634.5
46	90263.0
47	90263.0
48	97368.0
49	104473.0
50	115440.0
51	126407.0
52	131301.5
53	136196.0
54	136196.0
55	125424.0
56	114652.0
57	108768.0
58	102884.0
59	94995.5
60	87107.0
61	87107.0
62	79848.5
63	72590.0
64	61406.5
65	50223.0
66	43917.0
67	37611.0
68	37611.0
69	32163.0
70	26715.0
71	23081.0
72	19447.0
73	15791.0
74	12135.0
75	12135.0
76	9916.0
77	7697.0
78	6418.5
79	5140.0
80	4080.5
81	3021.0
82	3021.0
83	2464.5
84	1908.0
85	1549.5
86	1191.0
87	927.5
88	664.0
89	664.0
90	514.0
91	364.0
92	235.0
93	106.0
94	67.5
95	29.0
96	29.0
97	15.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1275182.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.284832419529714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.0609971644319	46.042544794390025
2	6.1067080301733165	6.385764119852166
3	1.8617137584442012	2.9201817562016363
4	0.8866834418581102	1.8544038066689246
5	0.5752076881711959	1.5037318791228043
6	0.3714686434072594	1.165330546179516
7	0.2802251616590805	1.0256067931956454
8	0.19505591641886813	0.8158772721918651
9	0.16151184285698822	0.7600157673792342
>10	1.1790867446432496	12.444505920657011
>50	0.1747309987463892	6.433160257298887
>100	0.13499639690411638	13.576234134421004
>500	0.008748367695499745	3.1083167396461606
>1k	0.0028658445899050885	1.9643262127951127
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2073	0.1625650299329821	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2039	0.15989874386558156	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1766	0.138490035147924	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1630	0.1278248908783217	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1500	0.11763026767943714	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1327	0.10406357680707537	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	7.842017845295808E-5	0.0	0.0	0.0	0.0
8	2.3526053535887425E-4	0.0	0.0	0.0	0.0
9	2.3526053535887425E-4	0.0	0.0	0.0	0.0
10	2.3526053535887425E-4	0.0	0.0	0.0	0.0
11	2.3526053535887425E-4	0.0	0.0	7.842017845295808E-5	0.0
12	2.3526053535887425E-4	0.0	0.0	2.3526053535887425E-4	0.0
13	2.3526053535887425E-4	0.0	0.0	2.3526053535887425E-4	0.0
14	2.3526053535887425E-4	0.0	0.0	3.1368071381183233E-4	0.0
15	2.3526053535887425E-4	0.0	0.0	3.921008922647904E-4	0.0
16	2.3526053535887425E-4	0.0	0.0	9.41042141435497E-4	0.0
17	2.3526053535887425E-4	0.0	0.0	0.0021173448182298686	0.0
18	2.3526053535887425E-4	0.0	0.0	0.0023526053535887427	0.0
19	3.1368071381183233E-4	0.0	0.0	0.002587865888947617	0.0
20	3.1368071381183233E-4	0.0	0.0	0.0032152273165712815	0.0
21	3.921008922647904E-4	0.0	0.0	0.005254151956348192	0.0
22	3.921008922647904E-4	0.0	0.0	0.008469379272919474	0.0
23	3.921008922647904E-4	0.0	0.0	0.01027304337733751	0.0
24	3.921008922647904E-4	0.0	0.0	0.013253010158549917	0.0
25	3.921008922647904E-4	0.0	0.0	0.013958791764626539	0.0
26	3.921008922647904E-4	0.0	0.0	0.015370354976779785	0.0
27	4.705210707177485E-4	0.0	0.0	0.025643398354117296	0.0
28	4.705210707177485E-4	0.0	0.0	0.07175446328445664	0.0
29	5.489412491707066E-4	0.0	0.0	0.1479788767407319	0.0
30	5.489412491707066E-4	0.0	0.0	0.25063089033565406	0.0
31	5.489412491707066E-4	0.0	0.0	0.5689383946762109	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1230	0.0	27.674797	1
ACGAACG	35	8.869931E-4	26.42857	31
GTTATCG	40	0.001931302	23.125002	11
TTCTGCG	570	0.0	21.745613	18
GTACTAT	70	5.1026473E-6	21.142857	1
GTAACGT	600	0.0	20.35	26
CTTATAC	930	0.0	20.091398	37
GGTAACG	620	0.0	19.693548	25
GGACCGT	255	0.0	19.588236	6
TGCGGGT	640	0.0	19.367188	21
TCTAATA	125	4.129106E-10	19.24	2
GACCGTT	155	1.8189894E-12	19.096775	7
GCGGTAA	855	0.0	19.040936	23
ATTGCCG	215	0.0	18.930233	11
TAATACG	865	0.0	18.82081	27
GACTAGT	70	1.2193303E-4	18.5	1
CAATACT	50	0.007035296	18.499998	4
ACGTCAA	645	0.0	18.35659	29
TAACGTC	670	0.0	18.22388	27
CGTCAAT	630	0.0	18.20635	30
>>END_MODULE