FastQCFastQC Report
Fri 10 Feb 2017
ERR1631821.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631821.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682071
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16750.24557560723150523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14790.2168395958778485No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12910.18927648294678998No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12440.18238570471402538No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA12140.17798733562928198No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA11230.16464561607222708No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA10410.1526234072405952No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10080.14778520124737748No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG9550.14001474919766418No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA9290.1362028293242199No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC8850.12975188799992962No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC8300.12168821134456677No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA8170.11978225140784464No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT8030.11772967916829771No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7610.11157196244965699No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT7480.10966600251293486No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA7290.10688036875926407No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7270.1065871441536145No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7250.10629391954796495No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6860.10057603973779856No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATAG250.005494796729.5999985
GGTATCA10050.028.348261
CGAACTA659.3801646E-825.61538524
TAGATAA400.001930386223.1250024
CTACCGT957.1322575E-921.4210538
ACCGTTT1001.2874807E-820.34999810
GTATCAA14850.019.1851842
TGCGGGT3700.019.021
TTCTGCG3900.018.518
TGTGCCG500.00703202218.49999810
GTTCCGG500.00703202218.4999981
TCTTATA12300.018.19918637
GCAGTCG2050.018.048789
CAGTCGG2100.017.61904710
AAAGCGA953.6020392E-617.52631620
GTTCTAG752.0657142E-417.2666661
GCTACCG1107.796152E-716.8181827
GTAGTTA1107.796152E-716.81818220
AGTCGGT2200.016.81818211
GCGAACT1005.8707446E-616.6523
Produced by FastQC (version 0.11.5)