FastQCFastQC Report
Fri 10 Feb 2017
ERR1631820.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631820.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862290
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA18400.21338528801215367No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA17770.2060791613030419No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA16440.19065511602825036No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA15520.17998585162764266No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA15200.17627480314047478No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14520.168388825105243No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13840.16050284707001125No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA12800.1484419394867156No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC12750.14786208816059562No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT12740.14774611789537162No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12520.1451947720604437No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA12080.14009208039058785No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG12020.13939625879924386No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC11710.13580118057729998No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG11580.13429356712938803No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC11540.13382968606849205No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA11350.1316262510292361No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT11060.1282631133377402No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA11020.1277992322768442No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA10780.1250159459114683No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC10770.1248999756462443No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA10760.12478400538102032No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10760.12478400538102032No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC10740.1245520648505723No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA10480.1215368379547484No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC10380.12037713530250845No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA10260.11898549211982047No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT10000.11597026522399657No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9160.10622876294518084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12750.030.4705891
ATACGGC1000.025.90000229
GTATCAA17950.021.6434522
TACGGCT1251.8189894E-1120.7230
GCGGTAA7100.019.02112823
CTTATAC6250.018.64837
AGACTAT500.007033489618.56
GTACCTA500.007033489618.51
GTATATC801.615907E-518.51
CTGGATA1002.8728573E-718.54
TAATACG7350.017.87074927
CGCGGTA7700.017.5389622
CGAACTA953.6042748E-617.52631624
TCTTATA12150.017.35802537
AGCCGCG7850.017.20382119
AACTCCG7850.017.2038215
CGTGCCA8200.017.14634310
TGCGGGT5400.017.1296321
CTAACTC8000.017.1125013
CGGTAAT7900.017.09493624
Produced by FastQC (version 0.11.5)