##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631820.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	862290
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.66400398937712	34.0	31.0	34.0	31.0	34.0
2	32.82882093031347	34.0	31.0	34.0	31.0	34.0
3	32.91429797399947	34.0	31.0	34.0	31.0	34.0
4	36.31888691739438	37.0	37.0	37.0	35.0	37.0
5	36.243447100163515	37.0	37.0	37.0	35.0	37.0
6	36.30083846501757	37.0	37.0	37.0	35.0	37.0
7	36.289067483097334	37.0	37.0	37.0	35.0	37.0
8	36.27219496920989	37.0	37.0	37.0	35.0	37.0
9	38.11130942026465	39.0	38.0	39.0	37.0	39.0
10	38.04960628094956	39.0	38.0	39.0	35.0	39.0
11	38.11986106762226	39.0	38.0	39.0	37.0	39.0
12	38.06150135105359	39.0	38.0	39.0	37.0	39.0
13	38.11043964327546	39.0	38.0	39.0	37.0	39.0
14	39.50999663686231	41.0	39.0	41.0	37.0	41.0
15	39.52417168238064	41.0	39.0	41.0	37.0	41.0
16	39.47343585104779	41.0	39.0	41.0	37.0	41.0
17	39.44419858748217	41.0	39.0	41.0	37.0	41.0
18	39.4707743334609	41.0	39.0	41.0	37.0	41.0
19	39.49095547901518	41.0	39.0	41.0	37.0	41.0
20	39.48653469250484	41.0	39.0	41.0	37.0	41.0
21	39.448575305291726	41.0	39.0	41.0	37.0	41.0
22	39.43001658374793	41.0	39.0	41.0	37.0	41.0
23	39.35784596829373	41.0	39.0	41.0	36.0	41.0
24	39.36933978128008	41.0	39.0	41.0	36.0	41.0
25	39.31725869487064	41.0	39.0	41.0	36.0	41.0
26	39.25631632049542	40.0	39.0	41.0	36.0	41.0
27	39.144941956882256	40.0	39.0	41.0	36.0	41.0
28	39.14022196708764	40.0	39.0	41.0	36.0	41.0
29	39.120607916130304	40.0	39.0	41.0	35.0	41.0
30	39.08102842431201	40.0	39.0	41.0	35.0	41.0
31	38.98974358974359	40.0	39.0	41.0	35.0	41.0
32	38.94698071414489	40.0	38.0	41.0	35.0	41.0
33	38.885500237739045	40.0	38.0	41.0	35.0	41.0
34	38.85476927715734	40.0	38.0	41.0	35.0	41.0
35	38.78277841561424	40.0	38.0	41.0	35.0	41.0
36	38.73168771526981	40.0	38.0	41.0	35.0	41.0
37	38.68472787577265	40.0	38.0	41.0	35.0	41.0
38	38.64150923703163	40.0	38.0	41.0	35.0	41.0
39	38.56404689837526	40.0	38.0	41.0	35.0	41.0
40	38.24315833420311	40.0	38.0	41.0	34.0	41.0
41	38.31813890918369	40.0	38.0	41.0	34.0	41.0
42	38.29770146934326	40.0	37.0	41.0	34.0	41.0
43	37.837156872977765	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	5.0
19	9.0
20	23.0
21	58.0
22	124.0
23	228.0
24	373.0
25	633.0
26	1016.0
27	1618.0
28	2522.0
29	3754.0
30	5272.0
31	7431.0
32	10085.0
33	13966.0
34	20718.0
35	31434.0
36	48154.0
37	87905.0
38	199746.0
39	427213.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.0079207691148	16.822994584188613	12.80276936993355	27.366315276763036
2	20.470607336278977	19.998724327082538	33.141634484918065	26.389033851720416
3	21.013464147792508	20.332138839601527	29.565111505410012	29.089285507195957
4	15.246610768998828	16.391817138085795	34.51959317630959	33.84197891660578
5	16.61853900659871	33.17039511069362	33.689362047571	16.52170383513667
6	33.24821115865892	34.17330596435074	16.120562687726867	16.457920189263472
7	29.752403483746768	28.342552969418637	20.435700286446558	21.469343260388037
8	26.751904811606302	31.07226107226107	20.826056199190525	21.349777916942095
9	27.900938199445662	13.332057660415868	18.337798188544458	40.429205951594014
10	19.18194574910993	23.798606037412007	30.435352375650883	26.58409583782718
11	38.026765937213696	20.283663268737897	19.63666515905322	22.052905634995188
12	22.29725498382215	24.111377842721126	27.53737141796843	26.05399575548829
13	34.25100604205082	17.977246633963052	21.86468589453664	25.90706142944949
14	23.622911085597654	20.60432105208225	24.049913602152408	31.722854260167693
15	28.98201301186376	24.564821579746955	20.940286910436164	25.512878497953125
16	24.78064224332881	24.56238620417725	23.28880075148732	27.36817080100662
17	25.907525310510387	24.285449210822346	22.829094620139394	26.97793085852787
18	25.938025490264295	22.040612786881443	24.304004453258184	27.71735726959608
19	27.31702791404284	22.97568103538253	24.125294274548008	25.581996776026628
20	28.800867457583873	21.27903605515546	23.908777789374806	26.01131869788586
21	26.73358150970091	22.963736098064455	22.919899337809785	27.38278305442485
22	27.407600691182783	23.511579630982617	22.225817300444167	26.855002377390434
23	27.22877454220738	22.80972758584699	23.319532871771678	26.641965000173958
24	26.98767236080669	22.374027299400435	23.587424184439108	27.050876155353766
25	27.444015354463115	22.650036530633546	23.9527305198947	25.95321759500864
26	27.207899894467058	23.36047037539575	23.275232230456112	26.156397499681084
27	26.616915422885572	22.560043604819725	24.606106994166694	26.21693397812801
28	25.788771758921015	23.75395748530077	22.96234445488177	27.49492630089645
29	26.42637627712255	24.078790198193182	23.06184694244396	26.432986582240314
30	26.313537209059596	23.00038270187524	24.348537035104197	26.337543053960964
31	27.119646522631598	22.994932099409713	23.554952510176392	26.330468867782304
32	24.88629115494787	23.03262243560751	23.843834440849363	28.23725196859525
33	25.23130269398926	22.616637094249032	25.5161256653794	26.635934546382305
34	25.485857426155935	22.673810435004466	24.64518897354718	27.195143165292418
35	25.458372473297846	24.158925651462965	25.06662491737119	25.316076957868
36	25.548829280172562	23.301441510396735	25.19535191176982	25.95437729766088
37	26.184926184926184	22.885688109568704	24.828885873661992	26.100499831843116
38	25.21437103526656	22.305024991592155	25.685790163402107	26.794813809739182
39	24.75478087418386	21.643414628489253	26.529705783437123	27.07209871388976
40	24.582333089795778	22.503450115390415	27.27852578598847	25.63569100882534
41	23.332173630681094	21.649329112015682	28.067819411102995	26.950677846200232
42	22.19636085307727	22.047686973060106	28.824177480893898	26.931774692968723
43	21.705806631179765	22.58694870635169	28.378619721903302	27.328624940565238
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12.0
1	13.0
2	14.0
3	38.5
4	63.0
5	63.0
6	95.0
7	127.0
8	133.0
9	139.0
10	218.5
11	298.0
12	298.0
13	612.5
14	927.0
15	1743.0
16	2559.0
17	2505.0
18	2451.0
19	2451.0
20	2881.5
21	3312.0
22	2856.0
23	2400.0
24	2692.5
25	2985.0
26	2985.0
27	3497.5
28	4010.0
29	5112.0
30	6214.0
31	7597.0
32	8980.0
33	8980.0
34	11357.5
35	13735.0
36	15306.5
37	16878.0
38	20483.5
39	24089.0
40	24089.0
41	28446.5
42	32804.0
43	37776.0
44	42748.0
45	55843.5
46	68939.0
47	68939.0
48	75384.0
49	81829.0
50	89468.5
51	97108.0
52	97814.0
53	98520.0
54	98520.0
55	89503.5
56	80487.0
57	75497.0
58	70507.0
59	63847.0
60	57187.0
61	57187.0
62	51665.5
63	46144.0
64	38360.0
65	30576.0
66	26604.0
67	22632.0
68	22632.0
69	19176.0
70	15720.0
71	13324.0
72	10928.0
73	8756.0
74	6584.0
75	6584.0
76	5439.0
77	4294.0
78	3457.5
79	2621.0
80	2083.0
81	1545.0
82	1545.0
83	1258.5
84	972.0
85	750.5
86	529.0
87	397.0
88	265.0
89	265.0
90	193.0
91	121.0
92	77.0
93	33.0
94	18.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	862290.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.27052344194009
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.5559352958584	39.58717909704126
2	7.178450008262839	6.643012787682415
3	2.20326337319807	3.058384486749879
4	1.1132614004819834	2.0604475091203467
5	0.6800530321100232	1.5733204882004632
6	0.46918842635482183	1.302575644820266
7	0.34431480983265533	1.1152138535838316
8	0.2686736587373716	0.9945336659871492
9	0.20701669337646764	0.8620893687373892
>10	1.6055887830370743	14.983988273196863
>50	0.20914977625848558	6.805669378048007
>100	0.14396328156309374	12.856574207635155
>500	0.014345991344471218	4.1999687388161835
>1k	0.006795469584223208	3.9570425003808434
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1840	0.21338528801215367	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1777	0.2060791613030419	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1644	0.19065511602825036	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1552	0.17998585162764266	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1520	0.17627480314047478	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1452	0.168388825105243	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1384	0.16050284707001125	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1280	0.1484419394867156	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1275	0.14786208816059562	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1274	0.14774611789537162	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1252	0.1451947720604437	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1208	0.14009208039058785	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1202	0.13939625879924386	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	1171	0.13580118057729998	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	1158	0.13429356712938803	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	1154	0.13382968606849205	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1135	0.1316262510292361	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1106	0.1282631133377402	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1102	0.1277992322768442	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	1078	0.1250159459114683	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1077	0.1248999756462443	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	1076	0.12478400538102032	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1076	0.12478400538102032	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	1074	0.1245520648505723	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1048	0.1215368379547484	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	1038	0.12037713530250845	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	1026	0.11898549211982047	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1000	0.11597026522399657	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	916	0.10622876294518084	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.1597026522399656E-4	0.0
12	0.0	0.0	0.0	1.1597026522399656E-4	0.0
13	0.0	0.0	0.0	1.1597026522399656E-4	0.0
14	0.0	0.0	0.0	1.1597026522399656E-4	0.0
15	0.0	0.0	0.0	1.1597026522399656E-4	0.0
16	0.0	1.1597026522399656E-4	0.0	4.6388106089598625E-4	0.0
17	0.0	1.1597026522399656E-4	0.0	8.11791856567976E-4	0.0
18	0.0	1.1597026522399656E-4	0.0	8.11791856567976E-4	0.0
19	0.0	1.1597026522399656E-4	0.0	8.11791856567976E-4	0.0
20	0.0	1.1597026522399656E-4	0.0	0.001855524243583945	0.0
21	1.1597026522399656E-4	1.1597026522399656E-4	0.0	0.00371104848716789	0.0
22	1.1597026522399656E-4	1.1597026522399656E-4	0.0	0.0052186619350798455	0.0
23	1.1597026522399656E-4	1.1597026522399656E-4	0.0	0.006494334852543808	0.0
24	1.1597026522399656E-4	1.1597026522399656E-4	0.0	0.00846582936135175	0.0
25	1.1597026522399656E-4	1.1597026522399656E-4	0.0	0.009973442809263705	0.0
26	1.1597026522399656E-4	1.1597026522399656E-4	0.0	0.011712996787623654	0.0
27	1.1597026522399656E-4	1.1597026522399656E-4	0.0	0.025745398879727238	0.0
28	1.1597026522399656E-4	1.1597026522399656E-4	0.0	0.09173247979218128	0.0
29	1.1597026522399656E-4	1.1597026522399656E-4	0.0	0.19842512379825814	0.0
30	1.1597026522399656E-4	1.1597026522399656E-4	0.0	0.3537093089331895	0.0
31	1.1597026522399656E-4	1.1597026522399656E-4	0.0	0.7794361525704809	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1275	0.0	30.470589	1
ATACGGC	100	0.0	25.900002	29
GTATCAA	1795	0.0	21.643452	2
TACGGCT	125	1.8189894E-11	20.72	30
GCGGTAA	710	0.0	19.021128	23
CTTATAC	625	0.0	18.648	37
AGACTAT	50	0.0070334896	18.5	6
GTACCTA	50	0.0070334896	18.5	1
GTATATC	80	1.615907E-5	18.5	1
CTGGATA	100	2.8728573E-7	18.5	4
TAATACG	735	0.0	17.870749	27
CGCGGTA	770	0.0	17.53896	22
CGAACTA	95	3.6042748E-6	17.526316	24
TCTTATA	1215	0.0	17.358025	37
AGCCGCG	785	0.0	17.203821	19
AACTCCG	785	0.0	17.203821	5
CGTGCCA	820	0.0	17.146343	10
TGCGGGT	540	0.0	17.12963	21
CTAACTC	800	0.0	17.112501	3
CGGTAAT	790	0.0	17.094936	24
>>END_MODULE