FastQCFastQC Report
Fri 10 Feb 2017
ERR1631819.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631819.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94990
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4260.4484682598168228No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3470.3653016106958627No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3390.3568796715443731No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA3100.3263501421202232No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2510.26423834087798714No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2460.25897462890830614No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2430.25581640172649756No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2420.25476365933256134No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2320.24423623539319927No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2240.23581429624170966No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2050.2158121907569218No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2020.21265396357511315No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1990.20949573639330457No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1950.20528476681755972No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1850.1947573428781977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1760.18528266133277185No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1710.18001894936309085No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1710.18001894936309085No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1700.17896620696915463No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1670.17580797978734602No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1670.17580797978734602No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1660.17475523739340984No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1620.17054426781766502No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1540.16212232866617537No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1520.16001684387830298No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1520.16001684387830298No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1520.16001684387830298No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1510.15896410148436677No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1480.15580587430255816No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1460.15370038951468576No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1450.15264764712074955No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1450.15264764712074955No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1440.15159490472681336No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1400.14738393515106854No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1400.14738393515106854No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1400.14738393515106854No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1350.1421202231813875No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC1350.1421202231813875No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1330.1400147383935151No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1330.1400147383935151No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1320.1389619959995789No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG1320.1389619959995789No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1300.1368565112117065No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1290.1358037688177703No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1260.13264554163596168No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA1260.13264554163596168No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1230.12948731445415307No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC1220.12843457206021688No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT1210.12738182966628067No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC1200.12632908727234446No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA1190.12527634487840825No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1180.12422360248447205No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1180.12422360248447205No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1180.12422360248447205No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1170.12317086009053585No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG1150.12106537530266344No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1130.11895989051479103No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA1100.11580166333298242No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC1090.11474892093904622No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA1080.11369617854511001No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC1060.11159069375723761No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1040.10948520896936519No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC1030.108432466575429No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA1020.10737972418149279No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA1020.10737972418149279No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT990.10422149699968418No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG990.10422149699968418No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT980.10316875460574797No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA980.10316875460574797No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC980.10316875460574797No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT970.10211601221181175No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA960.10106326981787557No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG950.10001052742393936No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT950.10001052742393936No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC950.10001052742393936No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTCA200.001833445737.019
GTTCAGC200.001833445737.021
TATGAAT200.001833445737.02
CGAGGGT200.001833445737.016
TCAGCTG200.001833445737.023
ACGAGGG200.001833445737.015
GCAAAGA200.001833445737.036
CTTACTT200.001833445737.033
TCTTACT200.001833445737.032
TGGCTCC200.001833445737.08
GGTATCA2150.031.8372081
ATGGCTC250.005471298529.67
CTCTTAC250.005471298529.631
ATGAATG250.005471298529.63
CACGAGG250.005471298529.614
GGTTCAG250.005471298529.620
AATGGCT250.005471298529.66
GAGGGTT250.005471298529.617
TGAATGG250.005471298529.64
CTTATAC950.029.21052737
Produced by FastQC (version 0.11.5)