##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631819.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	94990
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4537846089062	33.0	31.0	34.0	31.0	34.0
2	32.62966628066112	34.0	31.0	34.0	31.0	34.0
3	32.711980208442995	34.0	31.0	34.0	31.0	34.0
4	36.1723444573113	37.0	35.0	37.0	35.0	37.0
5	36.091041162227604	37.0	35.0	37.0	35.0	37.0
6	36.15981682282346	37.0	36.0	37.0	35.0	37.0
7	36.1492367617644	37.0	36.0	37.0	35.0	37.0
8	36.13790925360564	37.0	36.0	37.0	35.0	37.0
9	37.94728918833562	39.0	38.0	39.0	35.0	39.0
10	37.869870512685544	39.0	38.0	39.0	35.0	39.0
11	37.94721549636804	39.0	38.0	39.0	35.0	39.0
12	37.858100852721336	39.0	38.0	39.0	35.0	39.0
13	37.92047583956206	39.0	38.0	39.0	35.0	39.0
14	39.229708390356876	40.0	39.0	41.0	36.0	41.0
15	39.25132119170439	40.0	39.0	41.0	36.0	41.0
16	39.18531424360459	40.0	39.0	41.0	36.0	41.0
17	39.17693441414886	40.0	39.0	41.0	36.0	41.0
18	39.2198441941257	40.0	39.0	41.0	36.0	41.0
19	39.240046320665336	40.0	39.0	41.0	36.0	41.0
20	39.22715022633962	40.0	39.0	41.0	36.0	41.0
21	39.18588272449732	40.0	39.0	41.0	36.0	41.0
22	39.15034214127803	40.0	39.0	41.0	36.0	41.0
23	39.10872723444573	40.0	39.0	41.0	36.0	41.0
24	39.11098010316876	40.0	39.0	41.0	36.0	41.0
25	39.05153174018318	40.0	39.0	41.0	36.0	41.0
26	38.963764606800716	40.0	38.0	41.0	35.0	41.0
27	38.81801242236025	40.0	38.0	41.0	35.0	41.0
28	38.838730392672915	40.0	38.0	41.0	35.0	41.0
29	38.78926202758185	40.0	38.0	41.0	35.0	41.0
30	38.76547004947889	40.0	38.0	41.0	35.0	41.0
31	38.686672281292765	40.0	38.0	41.0	35.0	41.0
32	38.63165596378566	40.0	38.0	41.0	35.0	41.0
33	38.55240551637014	40.0	38.0	41.0	35.0	41.0
34	38.54162543425624	40.0	38.0	41.0	35.0	41.0
35	38.46642804505738	40.0	38.0	41.0	34.0	41.0
36	38.39985261606485	40.0	38.0	41.0	34.0	41.0
37	38.339172544478366	40.0	38.0	41.0	34.0	41.0
38	38.31453837246026	40.0	38.0	41.0	34.0	41.0
39	38.215475313190865	40.0	37.0	41.0	34.0	41.0
40	37.887356563848826	40.0	37.0	41.0	33.0	41.0
41	37.970344246762814	40.0	37.0	41.0	33.0	41.0
42	37.936667017580795	40.0	37.0	41.0	33.0	41.0
43	37.43101379092536	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	0.0
18	0.0
19	2.0
20	2.0
21	5.0
22	16.0
23	25.0
24	48.0
25	87.0
26	173.0
27	251.0
28	354.0
29	516.0
30	719.0
31	1038.0
32	1355.0
33	1836.0
34	2756.0
35	3952.0
36	6103.0
37	11384.0
38	26139.0
39	38228.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.2739235709022	17.106011159069375	13.284556269080955	27.335509000947468
2	21.51068533529845	20.298978839877883	32.24655226865986	25.943783556163808
3	20.94852089693652	19.990525318454573	30.42636066954416	28.634593115064742
4	14.34572060216865	17.460785345825876	36.958627223918306	31.234866828087167
5	16.033266659648383	33.135066849142014	34.36045899568376	16.471207495525846
6	29.77787135487946	36.29855774292031	17.107063901463313	16.81650700073692
7	27.909253605642697	28.706179597852405	20.582166543846718	22.802400252658174
8	25.814296241709656	30.481103274028847	21.39172544478366	22.312875039477838
9	28.008211390672706	12.61817033371934	18.130329508369304	41.24328876723865
10	19.778924097273396	22.76765975365828	29.068322981366464	28.385093167701864
11	38.53879355721655	20.01052742393936	19.038846194336244	22.411832824507844
12	21.334877355511107	24.975260553742498	27.029160964312034	26.66070112643436
13	35.792188651436994	18.206126960732707	20.46320665333193	25.538477734498365
14	23.08348247183914	21.125381619117803	24.71418044004632	31.076955468996736
15	30.17370249499947	23.946731234866828	20.832719233603537	25.046847036530163
16	23.29508369302032	24.83314033056111	24.075165806927046	27.796610169491526
17	26.637540793767766	24.15938519844194	21.78860932729761	27.414464680492685
18	26.113275081587535	21.103274028845142	23.858300873776187	28.925150015791136
19	27.737656595431098	22.528687230234762	24.040425307927148	25.693230866406992
20	30.48741972839246	19.824192020212653	23.45825876408043	26.230129487314453
21	26.8828297715549	21.966522791872826	22.54026739656806	28.610380040004213
22	28.06821770712706	23.4719444152016	21.55068954626803	26.909148331403305
23	27.77871354879461	21.938098747236552	23.12769765238446	27.15549005158438
24	27.16917570270555	21.235919570481105	23.804611011685438	27.790293715127905
25	27.740814822612908	22.19917886093273	23.82987682913991	26.230129487314453
26	27.552373934098327	23.34140435835351	23.080324244657334	26.02589746289083
27	26.138540899042006	22.046531213811978	25.930097905042636	25.88482998210338
28	25.607958732498158	23.778292451837036	22.480261080113696	28.13348773555111
29	26.789135698494576	24.39835772186546	22.896094325718497	25.916412253921468
30	26.219602063375092	22.00231603326666	25.28792504474155	26.490156858616697
31	27.949257816612278	22.36761764396252	23.681440151594906	26.0016843878303
32	24.53942520265291	22.35182650805348	23.796189072533952	29.31255921675966
33	24.943678281924413	21.803347720812717	26.06169070428466	27.19128329297821
34	25.39004105695336	22.4192020212654	24.90577955574271	27.284977366038532
35	24.898410358985156	24.59837877671334	25.220549531529635	25.282661332771873
36	25.705863775134226	22.832929782082324	25.965891146436466	25.49531529634698
37	26.14169912622381	22.988735656384883	25.20896936519634	25.660595852194966
38	25.03421412780293	21.941256974418362	26.173281398041897	26.851247499736814
39	24.553110853774083	21.068533529845247	27.099694704705758	27.278660911674912
40	24.560480050531634	22.3349826297505	27.572376039583112	25.53216128013475
41	22.585535319507315	21.03484577323929	28.869354668912518	27.510264238340877
42	20.525318454574165	22.12969786293294	29.918938835666914	27.42604484682598
43	20.08106116433309	22.94873144541531	29.04516264869986	27.92504474155174
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	2.0
2	3.0
3	5.5
4	8.0
5	8.0
6	6.0
7	4.0
8	9.5
9	15.0
10	27.0
11	39.0
12	39.0
13	76.5
14	114.0
15	205.0
16	296.0
17	299.0
18	302.0
19	302.0
20	314.0
21	326.0
22	225.0
23	124.0
24	116.5
25	109.0
26	109.0
27	131.5
28	154.0
29	175.0
30	196.0
31	278.5
32	361.0
33	361.0
34	615.0
35	869.0
36	951.0
37	1033.0
38	1462.5
39	1892.0
40	1892.0
41	2383.5
42	2875.0
43	3660.5
44	4446.0
45	6575.0
46	8704.0
47	8704.0
48	9786.0
49	10868.0
50	12128.0
51	13388.0
52	13073.0
53	12758.0
54	12758.0
55	11152.5
56	9547.0
57	8796.0
58	8045.0
59	7312.0
60	6579.0
61	6579.0
62	5821.5
63	5064.0
64	3820.5
65	2577.0
66	2142.0
67	1707.0
68	1707.0
69	1400.5
70	1094.0
71	908.5
72	723.0
73	516.5
74	310.0
75	310.0
76	260.5
77	211.0
78	167.0
79	123.0
80	94.0
81	65.0
82	65.0
83	48.5
84	32.0
85	24.0
86	16.0
87	12.0
88	8.0
89	8.0
90	6.0
91	4.0
92	2.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	94990.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.836930203179286
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.99724162821273	31.443309822086533
2	9.685236266144209	7.910306348036636
3	3.9287463586914493	4.813138225076323
4	2.1010028099301383	3.431940204232024
5	1.3070042020056198	2.6687019686282767
6	0.9821865896728623	2.4065691125381616
7	0.6444793895491222	1.8422991893883567
8	0.5233172643138873	1.709653647752395
9	0.43051223221881363	1.5822718180861144
>10	2.936248098785801	22.811874934203598
>50	0.2964605191925962	8.258764080429518
>100	0.1675646412827718	11.121170649542057
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	426	0.4484682598168228	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	347	0.3653016106958627	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	339	0.3568796715443731	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	310	0.3263501421202232	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	251	0.26423834087798714	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	246	0.25897462890830614	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	243	0.25581640172649756	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	242	0.25476365933256134	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	232	0.24423623539319927	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	224	0.23581429624170966	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	205	0.2158121907569218	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	202	0.21265396357511315	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	199	0.20949573639330457	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	195	0.20528476681755972	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	185	0.1947573428781977	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	176	0.18528266133277185	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	171	0.18001894936309085	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	171	0.18001894936309085	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	170	0.17896620696915463	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	167	0.17580797978734602	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	167	0.17580797978734602	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	166	0.17475523739340984	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	162	0.17054426781766502	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	154	0.16212232866617537	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	152	0.16001684387830298	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	152	0.16001684387830298	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	152	0.16001684387830298	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	151	0.15896410148436677	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	148	0.15580587430255816	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	146	0.15370038951468576	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	145	0.15264764712074955	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	145	0.15264764712074955	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	144	0.15159490472681336	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	140	0.14738393515106854	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	140	0.14738393515106854	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	140	0.14738393515106854	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	135	0.1421202231813875	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	135	0.1421202231813875	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	133	0.1400147383935151	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	133	0.1400147383935151	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	132	0.1389619959995789	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	132	0.1389619959995789	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	130	0.1368565112117065	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	129	0.1358037688177703	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	126	0.13264554163596168	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	126	0.13264554163596168	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	123	0.12948731445415307	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	122	0.12843457206021688	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	121	0.12738182966628067	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	120	0.12632908727234446	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	119	0.12527634487840825	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	118	0.12422360248447205	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	118	0.12422360248447205	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	118	0.12422360248447205	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	117	0.12317086009053585	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	115	0.12106537530266344	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	113	0.11895989051479103	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	110	0.11580166333298242	No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC	109	0.11474892093904622	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	108	0.11369617854511001	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	106	0.11159069375723761	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	104	0.10948520896936519	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC	103	0.108432466575429	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	102	0.10737972418149279	No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA	102	0.10737972418149279	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	99	0.10422149699968418	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	99	0.10422149699968418	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	98	0.10316875460574797	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	98	0.10316875460574797	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	98	0.10316875460574797	No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT	97	0.10211601221181175	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	96	0.10106326981787557	No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG	95	0.10001052742393936	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	95	0.10001052742393936	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	95	0.10001052742393936	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0010527423939362037	0.0
17	0.0	0.0	0.0	0.0010527423939362037	0.0
18	0.0	0.0	0.0	0.0021054847878724074	0.0
19	0.0	0.0	0.0	0.0031582271818086116	0.0
20	0.0	0.0	0.0	0.004210969575744815	0.0
21	0.0010527423939362037	0.0	0.0	0.004210969575744815	0.0
22	0.0010527423939362037	0.0	0.0	0.010527423939362039	0.0
23	0.0010527423939362037	0.0	0.0	0.015791135909043057	0.0
24	0.0010527423939362037	0.0	0.0	0.020002105484787872	0.0
25	0.0010527423939362037	0.0	0.0	0.021054847878724078	0.0
26	0.0010527423939362037	0.0	0.0	0.023160332666596485	0.0
27	0.0010527423939362037	0.0	0.0	0.03263501421202232	0.0
28	0.0010527423939362037	0.0	0.0	0.12106537530266344	0.0
29	0.0010527423939362037	0.0	0.0	0.2895041583324561	0.0
30	0.0010527423939362037	0.0	0.0	0.506369091483314	0.0
31	0.0010527423939362037	0.0	0.0	1.1285398462996106	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTTCA	20	0.0018334457	37.0	19
GTTCAGC	20	0.0018334457	37.0	21
TATGAAT	20	0.0018334457	37.0	2
CGAGGGT	20	0.0018334457	37.0	16
TCAGCTG	20	0.0018334457	37.0	23
ACGAGGG	20	0.0018334457	37.0	15
GCAAAGA	20	0.0018334457	37.0	36
CTTACTT	20	0.0018334457	37.0	33
TCTTACT	20	0.0018334457	37.0	32
TGGCTCC	20	0.0018334457	37.0	8
GGTATCA	215	0.0	31.837208	1
ATGGCTC	25	0.0054712985	29.6	7
CTCTTAC	25	0.0054712985	29.6	31
ATGAATG	25	0.0054712985	29.6	3
CACGAGG	25	0.0054712985	29.6	14
GGTTCAG	25	0.0054712985	29.6	20
AATGGCT	25	0.0054712985	29.6	6
GAGGGTT	25	0.0054712985	29.6	17
TGAATGG	25	0.0054712985	29.6	4
CTTATAC	95	0.0	29.210527	37
>>END_MODULE