FastQCFastQC Report
Fri 10 Feb 2017
ERR1631818.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631818.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1457145
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT71470.49047967086322913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT66000.4529405103816024No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT55250.3791661090694475No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA51310.35212693314666693No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA47870.32851912472677736No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA42370.29077408219497713No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG41890.2874799693922019No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC39470.2708721506782098No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT39030.26785254727566576No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC36600.2511761012116159No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA34160.23443102779750813No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT32870.22557809964004952No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA31750.21789183643357388No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA28850.1979899049168065No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA25150.17259778539541365No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA24160.1658036777396896No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG22610.15516643848072773No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA22460.15413702822986045No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC21400.14686252912373168No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT21220.14562723682269094No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA19800.13588215311448074No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC19180.13162725741089595No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT19120.13121549331054905No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG18890.12963706425921923No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA18820.1291566728088145No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA18620.12778412580765813No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT18330.1257939326559814No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC18300.12558805060580794No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA18170.1246958950550563No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG18170.1246958950550563No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA18010.12359785745413118No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC17800.12215668310291701No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC17690.121401782252281No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA17530.1203037446513559No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA16920.11611747629782898No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA16670.11440179254638351No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA16460.11296061819516934No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA15850.1087743498416424No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15730.10795082164094857No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT15140.10390180798753726No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC15050.10328416183701691No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT14760.10129396868534017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31250.029.0672021
AAGACGT502.7025843E-422.1999995
ATGCCCC1700.021.76470637
CTTATAC17200.020.7587237
GATTCTA450.00382629320.55555524
GTTATCG450.00382629320.55555511
CAGTCGG9400.020.46808410
TAGTTCC1550.020.2903234
GCAGTCG9500.020.2526329
AGTCGGT9700.020.02577411
TATACAC4900.020.01020437
GGGCAGT9750.019.9230777
TTTTACG1500.019.7333344
ATACACA2150.018.93023337
GTCGGTG10250.018.59024412
AGAGTGT609.238432E-418.56
TAACCGC609.238432E-418.522
GCAGCGT701.21948164E-418.51
ATACGGC1601.8189894E-1218.529
GTATTAT500.00703576618.4999981
Produced by FastQC (version 0.11.5)