FastQCFastQC Report
Fri 10 Feb 2017
ERR1631817.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631817.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238397
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5800.24329165216005233No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5620.23574122157577487No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5280.22147929713880626No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5060.212250993091356No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5000.20973418289659687No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4480.18792182787535078No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4170.17491830853576176No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3840.1610758524645864No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3840.1610758524645864No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3760.15772010553824084No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3670.15394489024610208No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3660.1535254218803089No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3610.15142808005134292No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3040.12751838320113087No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2900.12164582608002616No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2890.12122635771423299No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2890.12122635771423299No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2750.11535380059312828No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2600.10906177510623036No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.10864230674043718No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2590.10864230674043718No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2390.10025293942457329No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCCG250.00548771529.69
TTACTCG250.00548771529.629
ATGCCGT250.00548771529.634
ATACGGC556.226801E-726.9090929
TAAACCA358.848698E-426.4285725
GCTAAAC358.848698E-426.4285723
GGTATCA3350.025.9552231
GTACTTA751.3620593E-824.6666661
TTCTGCG1500.024.66666618
GGTAACG1450.024.24137925
GTAACGT1450.024.24137926
TACTTAG701.9096478E-723.7857152
GGCCGCA400.001926723223.12500233
AGCGACG400.001926723223.12500229
GCTCCGA400.001926723223.1250021
TACGGCT652.668059E-622.7692330
AATGAGC1300.022.7692334
TGCGGGT1550.022.67741821
TAACGTC1600.021.96875227
TCAATGA1350.021.92592432
Produced by FastQC (version 0.11.5)