##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631817.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	238397
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.528588866470635	34.0	31.0	34.0	31.0	34.0
2	32.69593157632017	34.0	31.0	34.0	31.0	34.0
3	32.78135630901396	34.0	31.0	34.0	31.0	34.0
4	36.24322453722152	37.0	37.0	37.0	35.0	37.0
5	36.1404338141839	37.0	35.0	37.0	35.0	37.0
6	36.196223106834395	37.0	36.0	37.0	35.0	37.0
7	36.190111452744794	37.0	36.0	37.0	35.0	37.0
8	36.169498777249714	37.0	36.0	37.0	35.0	37.0
9	38.00523915988876	39.0	38.0	39.0	35.0	39.0
10	37.90943677982525	39.0	38.0	39.0	35.0	39.0
11	37.99775164955935	39.0	38.0	39.0	35.0	39.0
12	37.91699559977684	39.0	38.0	39.0	35.0	39.0
13	37.97326308636434	39.0	38.0	39.0	35.0	39.0
14	39.332332202166974	40.0	39.0	41.0	36.0	41.0
15	39.34214356724288	40.0	39.0	41.0	36.0	41.0
16	39.27434070059606	40.0	39.0	41.0	36.0	41.0
17	39.25405101574265	40.0	39.0	41.0	36.0	41.0
18	39.26646308468647	40.0	39.0	41.0	36.0	41.0
19	39.28739036145589	40.0	39.0	41.0	36.0	41.0
20	39.2834305800828	40.0	39.0	41.0	36.0	41.0
21	39.237788227200845	40.0	39.0	41.0	36.0	41.0
22	39.22009085684803	40.0	39.0	41.0	36.0	41.0
23	39.164452572809225	40.0	39.0	41.0	36.0	41.0
24	39.174343636874625	40.0	39.0	41.0	36.0	41.0
25	39.11891508701871	40.0	39.0	41.0	36.0	41.0
26	39.05823479322307	40.0	39.0	41.0	36.0	41.0
27	38.93359815769494	40.0	38.0	41.0	35.0	41.0
28	38.934898509628894	40.0	38.0	41.0	35.0	41.0
29	38.90306086066519	40.0	38.0	41.0	35.0	41.0
30	38.87285913832808	40.0	38.0	41.0	35.0	41.0
31	38.788239784896625	40.0	38.0	41.0	35.0	41.0
32	38.74475349941484	40.0	38.0	41.0	35.0	41.0
33	38.68331396787711	40.0	38.0	41.0	35.0	41.0
34	38.65078839079351	40.0	38.0	41.0	35.0	41.0
35	38.58405097379581	40.0	38.0	41.0	35.0	41.0
36	38.549214126016686	40.0	38.0	41.0	35.0	41.0
37	38.498110295012104	40.0	38.0	41.0	34.0	41.0
38	38.463151801406894	40.0	38.0	41.0	34.0	41.0
39	38.40214012760228	40.0	38.0	41.0	34.0	41.0
40	38.09232918199474	40.0	37.0	41.0	34.0	41.0
41	38.17730927822078	40.0	37.0	41.0	34.0	41.0
42	38.167061666044454	40.0	37.0	41.0	34.0	41.0
43	37.677244260624086	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	1.0
18	3.0
19	7.0
20	8.0
21	29.0
22	39.0
23	84.0
24	130.0
25	229.0
26	344.0
27	529.0
28	819.0
29	1241.0
30	1690.0
31	2269.0
32	3196.0
33	4463.0
34	6368.0
35	9626.0
36	14755.0
37	26386.0
38	61307.0
39	104873.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.4132854020814	16.36807510161621	13.099997063721439	28.11864243258095
2	20.931471453080366	19.578685973397317	32.25460051930183	27.235242054220482
3	21.00739522728893	19.551001061254965	29.519666774330215	29.92193693712589
4	15.564373712756453	15.94063683687295	34.10655335427879	34.38843609609181
5	16.897863647613015	32.605276073104946	33.698410634362006	16.798449644920026
6	33.72525660977278	33.72945129343071	15.78585300989526	16.759439086901263
7	30.24660545224982	27.41225770458521	20.096729405151912	22.244407438013063
8	27.361502032324232	30.540652776670846	20.532137568845247	21.565707622159675
9	28.248677625976836	12.743448952797227	17.776230405583963	41.23164301564198
10	19.26282629395504	23.323280074833157	29.476881001019308	27.93701263019249
11	39.039501336006744	19.683972533211406	18.644110454410082	22.632415676371767
12	22.671845702756325	23.94744900313343	26.594294391288482	26.78641090282176
13	35.263866575502206	17.682269491646288	20.822409677974136	26.231454254877367
14	24.1219478433034	20.170975305897308	23.203312122216303	32.50376472858299
15	29.82378133953028	24.004916169247096	19.98263400965616	26.188668481566463
16	25.259965519700334	24.212133541948937	22.798944617591665	27.728956320759067
17	27.179872229935782	23.556504486214173	21.75153210820606	27.512091175643988
18	26.646308468646833	21.311090324123207	23.487711674224087	28.554889533005866
19	28.115706154020394	22.09885191508282	23.52840010570603	26.257041825190754
20	30.09559684056427	20.670981597922793	22.80985079510229	26.423570766410652
21	27.27844729589718	22.329978984634874	21.975528215539626	28.416045503928324
22	28.52468781066876	22.84424720109733	21.342130983191904	27.28893400504201
23	28.121159242775708	22.134506726175246	22.315717060197905	27.428616970851145
24	27.749090802317145	21.631983623955	23.082924701233658	27.5360008724942
25	28.056561114443557	21.95497426561576	23.077891080844136	26.91057353909655
26	27.924428579218695	22.814884415491807	22.336271010121774	26.92441599516772
27	26.839263916911705	21.618141167883824	24.426481876869254	27.116113038335214
28	26.34303284017836	22.927721405890175	22.34927452946136	28.379971224470108
29	27.205040331883374	23.460865698813326	22.52754858492347	26.806545384379838
30	26.653439430865323	22.246924248207822	24.04602406909483	27.05361225183203
31	27.911425059879107	22.37695944160371	22.9692487741037	26.74236672441348
32	25.437820106796643	22.337948883584943	23.188630729413543	29.03560028020487
33	25.837992927763352	21.942390214641964	24.703750466658555	27.51586639093613
34	26.477682185597974	21.647923421855143	24.261211340746737	27.61318305180015
35	25.69621262012525	23.394589697018002	24.7750600888434	26.134137594013346
36	25.94034320901689	22.014538773558392	25.237733696313292	26.80738432111142
37	26.517112211982536	21.781314362177376	24.96424032181613	26.737333104023957
38	25.656782593740697	21.05185887406301	26.191604760127014	27.099753772069278
39	25.042261437853664	20.049748948183073	27.226852686904618	27.681136927058645
40	24.777157430672368	20.921823680667124	28.029715139032792	26.271303749627723
41	22.952470039471972	20.179784141578963	29.085517015734258	27.782228803214803
42	21.509079392777593	20.75193899252088	30.074203953908818	27.664777660792712
43	20.607222406322226	21.61101020566534	29.588459586320297	28.193307801692136
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	2.0
2	3.0
3	8.0
4	13.0
5	13.0
6	11.5
7	10.0
8	14.0
9	18.0
10	30.5
11	43.0
12	43.0
13	92.5
14	142.0
15	300.0
16	458.0
17	429.5
18	401.0
19	401.0
20	476.5
21	552.0
22	468.5
23	385.0
24	433.5
25	482.0
26	482.0
27	580.0
28	678.0
29	912.5
30	1147.0
31	1390.0
32	1633.0
33	1633.0
34	2066.5
35	2500.0
36	2898.0
37	3296.0
38	4339.0
39	5382.0
40	5382.0
41	6411.5
42	7441.0
43	8737.0
44	10033.0
45	14007.0
46	17981.0
47	17981.0
48	20106.0
49	22231.0
50	25330.0
51	28429.0
52	29065.0
53	29701.0
54	29701.0
55	26637.5
56	23574.0
57	22330.5
58	21087.0
59	19399.5
60	17712.0
61	17712.0
62	16084.0
63	14456.0
64	11903.5
65	9351.0
66	8016.5
67	6682.0
68	6682.0
69	5628.0
70	4574.0
71	3908.0
72	3242.0
73	2568.0
74	1894.0
75	1894.0
76	1544.5
77	1195.0
78	955.5
79	716.0
80	573.5
81	431.0
82	431.0
83	341.0
84	251.0
85	198.5
86	146.0
87	116.5
88	87.0
89	87.0
90	60.5
91	34.0
92	19.0
93	4.0
94	2.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	238397.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.33117646157696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.55201898956147	39.898361970439474
2	7.807978014340482	7.547375264384061
3	2.7844109300663247	4.037215680077374
4	1.5403207376827492	2.9778205352148532
5	0.9423957670736396	2.2773548057539625
6	0.6710391901254579	1.9459268106352319
7	0.5028946677975482	1.7013843651636595
8	0.4064604772640117	1.571577044104299
9	0.32297576646423004	1.4048818885616192
>10	2.1813137477308393	20.267621686219726
>50	0.17887761427857307	5.9191936068821
>100	0.10533903951960415	9.40615770830729
>500	0.003975058095079401	1.0451286342563655
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	580	0.24329165216005233	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	562	0.23574122157577487	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	528	0.22147929713880626	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	506	0.212250993091356	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	500	0.20973418289659687	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	448	0.18792182787535078	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	417	0.17491830853576176	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	384	0.1610758524645864	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	384	0.1610758524645864	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	376	0.15772010553824084	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	367	0.15394489024610208	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	366	0.1535254218803089	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	361	0.15142808005134292	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	304	0.12751838320113087	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	290	0.12164582608002616	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	289	0.12122635771423299	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	289	0.12122635771423299	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	275	0.11535380059312828	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	260	0.10906177510623036	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	259	0.10864230674043718	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	259	0.10864230674043718	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	239	0.10025293942457329	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	4.194683657931937E-4	0.0	4.194683657931937E-4	0.0
11	0.0	4.194683657931937E-4	0.0	4.194683657931937E-4	0.0
12	0.0	4.194683657931937E-4	0.0	4.194683657931937E-4	0.0
13	0.0	4.194683657931937E-4	0.0	4.194683657931937E-4	0.0
14	0.0	4.194683657931937E-4	0.0	4.194683657931937E-4	0.0
15	0.0	4.194683657931937E-4	0.0	4.194683657931937E-4	0.0
16	0.0	4.194683657931937E-4	0.0	4.194683657931937E-4	0.0
17	0.0	4.194683657931937E-4	0.0	4.194683657931937E-4	0.0
18	0.0	4.194683657931937E-4	0.0	8.389367315863874E-4	0.0
19	0.0	4.194683657931937E-4	0.0	0.0016778734631727747	0.0
20	0.0	4.194683657931937E-4	0.0	0.0025168101947591623	0.0
21	0.0	4.194683657931937E-4	0.0	0.007550430584277487	0.0
22	0.0	4.194683657931937E-4	0.0	0.010486709144829843	0.0
23	0.0	4.194683657931937E-4	0.0	0.011745114242209424	0.0
24	0.0	4.194683657931937E-4	0.0	0.012164582608002617	0.0
25	0.0	4.194683657931937E-4	0.0	0.013003519339589004	0.0
26	0.0	4.194683657931937E-4	0.0	0.020553949923866492	0.0
27	0.0	4.194683657931937E-4	0.0	0.044883115139871727	0.0
28	0.0	4.194683657931937E-4	0.0	0.15058914331975654	0.0
29	0.0	4.194683657931937E-4	0.0	0.33263841407400263	0.0
30	0.0	4.194683657931937E-4	0.0	0.5604097366997068	0.0
31	0.0	4.194683657931937E-4	0.0	1.1753503609525289	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCCG	25	0.005487715	29.6	9
TTACTCG	25	0.005487715	29.6	29
ATGCCGT	25	0.005487715	29.6	34
ATACGGC	55	6.226801E-7	26.90909	29
TAAACCA	35	8.848698E-4	26.428572	5
GCTAAAC	35	8.848698E-4	26.428572	3
GGTATCA	335	0.0	25.955223	1
GTACTTA	75	1.3620593E-8	24.666666	1
TTCTGCG	150	0.0	24.666666	18
GGTAACG	145	0.0	24.241379	25
GTAACGT	145	0.0	24.241379	26
TACTTAG	70	1.9096478E-7	23.785715	2
GGCCGCA	40	0.0019267232	23.125002	33
AGCGACG	40	0.0019267232	23.125002	29
GCTCCGA	40	0.0019267232	23.125002	1
TACGGCT	65	2.668059E-6	22.76923	30
AATGAGC	130	0.0	22.76923	34
TGCGGGT	155	0.0	22.677418	21
TAACGTC	160	0.0	21.968752	27
TCAATGA	135	0.0	21.925924	32
>>END_MODULE