FastQCFastQC Report
Fri 10 Feb 2017
ERR1631816.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631816.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689277
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA18540.26897749380873004No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA16490.23923618516213363No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC15800.22922569591035244No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA15130.21950536576731852No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG13790.20006470548125066No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA13060.18947389801197487No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT12460.18076912474955642No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC12440.18047896564080912No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12350.17917324965144638No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT11540.16742180574718146No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA10470.1518982934292019No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9650.14000176997056335No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA8490.12317254166322102No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA8350.12114142790199006No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA7660.11113093865020883No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT7300.10590807469275777No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7200.10445727914902135No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG6980.10126552895280128No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6920.10039505162655943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9800.028.6938761
AAACCGT358.8657794E-426.428576
TAGCGAA751.3716999E-824.66666610
GCGAAAT802.7217538E-823.12512
AGTATTA400.001930407423.12515
ATCCCTT400.001930407423.1255
GACTGGA400.001930407423.1257
TAAGGTA903.8216967E-922.6111135
TATATCA502.7005823E-422.1999992
CTACCGT502.7005823E-422.1999998
TCGGGTA855.173206E-821.76470625
TAATACA603.7235015E-521.5833324
TTAGCTA603.7235015E-521.5833324
GCGGTAA5450.021.04587223
ACACGAA806.9517955E-720.812517
ACGAAAG806.9517955E-720.812519
AGTCGGT2500.020.72000111
GTAGCGA909.464384E-820.5555579
CTTATAC6700.020.15671737
GACCGTT656.897007E-519.9230777
Produced by FastQC (version 0.11.5)