##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631816.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	689277
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63651333208565	34.0	31.0	34.0	31.0	34.0
2	32.803492645191994	34.0	31.0	34.0	31.0	34.0
3	32.88917953159615	34.0	31.0	34.0	31.0	34.0
4	36.30417524449532	37.0	37.0	37.0	35.0	37.0
5	36.22513880486365	37.0	35.0	37.0	35.0	37.0
6	36.2789676719229	37.0	37.0	37.0	35.0	37.0
7	36.27462689165604	37.0	37.0	37.0	35.0	37.0
8	36.25581587663596	37.0	37.0	37.0	35.0	37.0
9	38.09780102919436	39.0	38.0	39.0	37.0	39.0
10	38.022577280251625	39.0	38.0	39.0	35.0	39.0
11	38.10380877354097	39.0	38.0	39.0	37.0	39.0
12	38.03622201234047	39.0	38.0	39.0	35.0	39.0
13	38.0906805246657	39.0	38.0	39.0	37.0	39.0
14	39.48510540755023	40.0	39.0	41.0	37.0	41.0
15	39.501493594012274	41.0	39.0	41.0	37.0	41.0
16	39.444111728666414	40.0	39.0	41.0	37.0	41.0
17	39.41083918366636	40.0	39.0	41.0	37.0	41.0
18	39.428122510978895	41.0	39.0	41.0	37.0	41.0
19	39.4557775756336	41.0	39.0	41.0	37.0	41.0
20	39.456218617478896	41.0	39.0	41.0	37.0	41.0
21	39.41366243179448	40.0	39.0	41.0	37.0	41.0
22	39.394942816893646	40.0	39.0	41.0	37.0	41.0
23	39.32872705748197	40.0	39.0	41.0	36.0	41.0
24	39.34711734179437	40.0	39.0	41.0	36.0	41.0
25	39.297176606792334	40.0	39.0	41.0	36.0	41.0
26	39.23874871785944	40.0	39.0	41.0	36.0	41.0
27	39.1299288965104	40.0	39.0	41.0	36.0	41.0
28	39.128359135732076	40.0	39.0	41.0	36.0	41.0
29	39.11156327572224	40.0	39.0	41.0	35.0	41.0
30	39.080004120259346	40.0	39.0	41.0	35.0	41.0
31	38.99417070350527	40.0	39.0	41.0	35.0	41.0
32	38.956657483130876	40.0	38.0	41.0	35.0	41.0
33	38.8983862801167	40.0	38.0	41.0	35.0	41.0
34	38.87545935813903	40.0	38.0	41.0	35.0	41.0
35	38.815281809780394	40.0	38.0	41.0	35.0	41.0
36	38.772391941120915	40.0	38.0	41.0	35.0	41.0
37	38.72946870416393	40.0	38.0	41.0	35.0	41.0
38	38.68914674361686	40.0	38.0	41.0	35.0	41.0
39	38.621713766743994	40.0	38.0	41.0	35.0	41.0
40	38.30638625690397	40.0	38.0	41.0	34.0	41.0
41	38.39325989406291	40.0	38.0	41.0	34.0	41.0
42	38.39746720113975	40.0	38.0	41.0	34.0	41.0
43	37.93224929890305	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	4.0
19	10.0
20	19.0
21	37.0
22	92.0
23	170.0
24	276.0
25	492.0
26	802.0
27	1234.0
28	1931.0
29	2922.0
30	4208.0
31	5763.0
32	8183.0
33	11249.0
34	16662.0
35	24975.0
36	38819.0
37	70176.0
38	163005.0
39	338246.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.42909599478874	16.555318108684897	13.064994189563848	27.950591706962513
2	20.22104320904368	19.55135598605495	33.159528027193716	27.06807277770766
3	20.793817289710816	19.871256403448832	29.87086468865202	29.46406161818833
4	15.325913964922666	16.08685622761241	34.215997342142565	34.37123246532236
5	16.408207440550026	33.19391188158026	33.98996339642843	16.40791728144128
6	33.808904112570126	33.795411714013376	15.968616390797893	16.4270677826186
7	30.05932302978338	28.245538441004125	20.274722644161926	21.420415885050566
8	27.15308939657061	31.22837407892618	20.563721116474216	21.054815408028993
9	28.519738798770305	12.940225772802517	17.90035065728292	40.63968477114426
10	18.85947159124706	24.106853993387272	29.983881661509088	27.04979275385658
11	38.69199175367813	19.665388515792635	19.2076625217438	22.434957208785438
12	22.48529981415309	24.242503376726628	26.721622801863404	26.550574007256877
13	34.455668765967815	17.94416468270376	21.319440515206516	26.28072603612191
14	23.725149685830225	20.423139028286162	23.409891814176305	32.44181947170731
15	29.344225906275707	24.749701498816876	20.352775444414945	25.553297150492472
16	25.33480734160577	24.53948122452947	22.719893453575267	27.40581798028949
17	26.668523684962647	24.344929542114418	21.88438029993747	27.102166472985463
18	26.262591091825204	21.951116894949347	23.655511499730878	28.130780513494575
19	27.767356229788604	22.802733879122616	23.670599773385735	25.759310117703045
20	29.53340964517893	21.09572784236236	23.393497824532083	25.977364687926624
21	26.793001942615234	22.845242188554092	22.29437512059738	28.067380748233294
22	28.226242860272432	23.115525398352187	21.72450263101772	26.933729110357664
23	27.223452980441827	22.753696989744327	22.81405008436376	27.208799945450085
24	27.56119818302366	22.21429120658313	23.393497824532083	26.831012785861123
25	27.498668895088624	22.37924665990596	23.455736953358375	26.666347491647045
26	27.407413855387603	23.1435257523463	22.860910780426448	26.58814961183965
27	26.52939239231833	22.022786194809925	24.547605679574396	26.900215733297355
28	25.660510941174593	23.14439622967254	22.764432876767977	28.43065995238489
29	26.973771067364787	23.796818985690805	22.77850559354222	26.450904353402187
30	26.32628101619523	22.684348962753724	23.775927529861	27.213442491190044
31	27.625033186948063	22.88064159982126	23.25509192965963	26.239233283571046
32	25.403139811715754	22.761096047017382	23.311237717202225	28.524526424064632
33	25.57796067473599	22.147264452462508	24.90812837219289	27.36664650060861
34	25.970110710207944	22.247949663197815	24.383230544469058	27.398709082125183
35	25.36237245693676	23.847596829721578	24.743317998424434	26.046712714917224
36	25.882917898029383	22.65025526747592	25.21177987949692	26.255046954997773
37	26.49355774238804	22.24577346988221	24.78742218295402	26.47324660477573
38	25.550395559404997	21.72174611948462	25.86986073813576	26.857997582974626
39	25.097602270204867	20.655266315283985	26.86293028782333	27.384201126687817
40	24.752312930795604	21.63092631844672	27.647230358767228	25.969530391990446
41	22.94824867215938	20.989239449452107	28.64769896572786	27.414812912660658
42	21.817208466262475	21.225864202635517	29.346837338254435	27.61008999284758
43	20.881445340552492	21.97955248760658	29.366132918986125	27.772869252854804
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	4.0
2	5.0
3	13.5
4	22.0
5	22.0
6	34.5
7	47.0
8	55.0
9	63.0
10	105.5
11	148.0
12	148.0
13	272.5
14	397.0
15	877.5
16	1358.0
17	1279.0
18	1200.0
19	1200.0
20	1381.0
21	1562.0
22	1433.0
23	1304.0
24	1491.0
25	1678.0
26	1678.0
27	1961.5
28	2245.0
29	3254.0
30	4263.0
31	4876.5
32	5490.0
33	5490.0
34	7076.0
35	8662.0
36	9723.5
37	10785.0
38	14516.5
39	18248.0
40	18248.0
41	21617.5
42	24987.0
43	28042.0
44	31097.0
45	42850.5
46	54604.0
47	54604.0
48	60896.0
49	67188.0
50	76080.5
51	84973.0
52	86065.0
53	87157.0
54	87157.0
55	75979.5
56	64802.0
57	60180.0
58	55558.0
59	50809.0
60	46060.0
61	46060.0
62	41492.0
63	36924.0
64	30737.5
65	24551.0
66	21371.0
67	18191.0
68	18191.0
69	15440.0
70	12689.0
71	10845.5
72	9002.0
73	7177.5
74	5353.0
75	5353.0
76	4415.0
77	3477.0
78	2828.0
79	2179.0
80	1739.0
81	1299.0
82	1299.0
83	1076.0
84	853.0
85	658.0
86	463.0
87	342.5
88	222.0
89	222.0
90	175.0
91	128.0
92	81.0
93	34.0
94	19.5
95	5.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	689277.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.0707326232532
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.83629609391646	39.54541046713217
2	6.642956802445737	6.120917733465972
3	2.2383706277175714	3.0937012410395903
4	1.1353085059220291	2.092179784849555
5	0.6931136347104148	1.5966126471137352
6	0.5015290156028994	1.3863485508386735
7	0.3471477801866753	1.1195346793215335
8	0.27719937246991155	1.0216622537915896
9	0.211641801547269	0.8775443565889061
>10	1.6997310370507466	16.025817447011683
>50	0.23178442514212785	7.387698006759546
>100	0.17098850646511568	14.496325693248972
>500	0.0104492976173126	3.017837091467743
>1k	0.003483099205770867	2.2184100473703277
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1854	0.26897749380873004	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1649	0.23923618516213363	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1580	0.22922569591035244	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1513	0.21950536576731852	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1379	0.20006470548125066	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1306	0.18947389801197487	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1246	0.18076912474955642	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1244	0.18047896564080912	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1235	0.17917324965144638	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1154	0.16742180574718146	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1047	0.1518982934292019	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	965	0.14000176997056335	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	849	0.12317254166322102	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	835	0.12114142790199006	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	766	0.11113093865020883	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	730	0.10590807469275777	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	720	0.10445727914902135	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	698	0.10126552895280128	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	692	0.10039505162655943	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.450795543736408E-4	0.0	0.0	0.0	0.0
8	2.901591087472816E-4	0.0	0.0	0.0	0.0
9	2.901591087472816E-4	0.0	0.0	0.0	0.0
10	2.901591087472816E-4	0.0	0.0	0.0	0.0
11	2.901591087472816E-4	0.0	0.0	0.0	0.0
12	2.901591087472816E-4	0.0	0.0	0.0	0.0
13	2.901591087472816E-4	0.0	0.0	0.0	0.0
14	4.3523866312092233E-4	0.0	0.0	2.901591087472816E-4	0.0
15	4.3523866312092233E-4	0.0	0.0	4.3523866312092233E-4	0.0
16	4.3523866312092233E-4	0.0	0.0	0.0015958750981100486	0.0
17	4.3523866312092233E-4	0.0	0.0	0.002611431978725534	0.0
18	5.803182174945632E-4	0.0	0.0	0.0030466706418464566	0.0
19	5.803182174945632E-4	0.0	0.0	0.004207307076835583	0.0
20	5.803182174945632E-4	0.0	0.0	0.0055130230661983495	0.0
21	5.803182174945632E-4	0.0	0.0	0.007979375490550243	0.0
22	5.803182174945632E-4	0.0	0.0	0.011606364349891263	0.0
23	5.803182174945632E-4	0.0	0.0	0.014362875882990438	0.0
24	5.803182174945632E-4	0.0	0.0	0.017699705633584174	0.0
25	5.803182174945632E-4	0.0	0.0	0.019150501177320582	0.0
26	5.803182174945632E-4	0.0	0.0	0.021907012710419758	0.0
27	5.803182174945632E-4	0.0	0.0	0.03786576369152025	0.0
28	5.803182174945632E-4	0.0	0.0	0.11969063235825365	0.0
29	7.253977718682039E-4	0.0	0.0	0.2678168573737409	0.0
30	7.253977718682039E-4	0.0	0.0	0.4475704252426818	1.450795543736408E-4
31	7.253977718682039E-4	0.0	0.0	0.975369844053987	1.450795543736408E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	980	0.0	28.693876	1
AAACCGT	35	8.8657794E-4	26.42857	6
TAGCGAA	75	1.3716999E-8	24.666666	10
GCGAAAT	80	2.7217538E-8	23.125	12
AGTATTA	40	0.0019304074	23.125	15
ATCCCTT	40	0.0019304074	23.125	5
GACTGGA	40	0.0019304074	23.125	7
TAAGGTA	90	3.8216967E-9	22.611113	5
TATATCA	50	2.7005823E-4	22.199999	2
CTACCGT	50	2.7005823E-4	22.199999	8
TCGGGTA	85	5.173206E-8	21.764706	25
TAATACA	60	3.7235015E-5	21.583332	4
TTAGCTA	60	3.7235015E-5	21.583332	4
GCGGTAA	545	0.0	21.045872	23
ACACGAA	80	6.9517955E-7	20.8125	17
ACGAAAG	80	6.9517955E-7	20.8125	19
AGTCGGT	250	0.0	20.720001	11
GTAGCGA	90	9.464384E-8	20.555557	9
CTTATAC	670	0.0	20.156717	37
GACCGTT	65	6.897007E-5	19.923077	7
>>END_MODULE