FastQCFastQC Report
Fri 10 Feb 2017
ERR1631815.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631815.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9058
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC250.27599911680282624No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA180.1987193640980349No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG170.18767939942592185No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT160.17663943475380878No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC150.16559947008169573No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG150.16559947008169573No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT150.16559947008169573No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC140.1545595054095827No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA140.1545595054095827No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC130.14351954073746964No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGTGGG130.14351954073746964No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA120.1324795760653566No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG120.1324795760653566No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA120.1324795760653566No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA110.12143961139324355No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT110.12143961139324355No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT110.12143961139324355No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT100.11039964672113049No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG100.11039964672113049No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG100.11039964672113049No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA100.11039964672113049No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA100.11039964672113049No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG100.11039964672113049No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA100.11039964672113049No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG100.11039964672113049No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAA250.0052166129.618
Produced by FastQC (version 0.11.5)